################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NarI NciI NcoI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AspHI 383 GTGCTC G[AT]GC[AT]^C BanII 199 GGGCTC G[GA]GC[CT]^C BbsI 175 GTCTTC GAAGAC(2/6) BglI 154 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 175 GTCTTC GAAGAC(2/6) BsaI 518 GAGACC GGTCTC(1/5) BsiHKAI 383 GTGCTC G[AT]GC[AT]^C BsmAI 518 GAGAC GTCTC(1/5) BspDI 749 ATCGAT AT^CGAT BsrFI 159 GCCGGC [GA]^CCGG[CT] BstAPI 661 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 54 GGTCACC G^GT[ACGT]ACC BstPI 54 GGTCACC G^GT[ACGT]ACC CfoI 26 GCGC GCG^C Cfr10I 159 GCCGGC [GA]^CCGG[CT] ClaI 749 ATCGAT AT^CGAT DdeI 205 CTGAG C^T[ACGT]AG EaeI 69 TGGCCG [CT]^GGCC[GA] EcoO65I 54 GGTCACC G^GT[ACGT]ACC HaeII 25 AGCGCC [GA]GCGC^[CT] HapII 160 CCGG C^CGG HhaI 26 GCGC GCG^C HinP1I 26 GCGC G^CGC HindIII 413 AAGCTT A^AGCTT HpaII 160 CCGG C^CGG MspI 160 CCGG C^CGG NaeI 159 GCCGGC GCC^GGC NgoMIV 159 GCCGGC G^CCGGC NheI 733 GCTAGC G^CTAGC NlaIII 138 CATG CATG^ PleI 481 GAGTC GAGTC(4/5) SspI 718 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 230 AAATTC [GA]^AATT[CT] 571 AAATTC ApoI 230 AAATTC [GA]^AATT[CT] 571 AAATTC BbvI 109 GCAGC GCAGC(8/12) 397 GCAGC BfaI 478 CTAG C^TAG 734 CTAG BmyI 199 GGGCTC G[AGT]GC[ACT]^C 383 GTGCTC BseRI 37 GAGGAG GAGGAG(10/8) 148 GAGGAG Bsp1286I 199 GGGCTC G[AGT]GC[ACT]^C 383 GTGCTC BsrI 272 ACTGG ACTGG(1/-1) 235 CCAGT Cfr13I 132 GGGCC G^G[ACGT]CC 170 GGCCC DpnII 740 GATC ^GATC 748 GATC EarI 472 CTCTTC CTCTTC(1/4) 165 GAAGAG HphI 56 TCACC GGTGA(8/7) 575 TCACC Ksp632I 472 CTCTTC CTCTTC(1/4) 165 GAAGAG MaeI 478 CTAG C^TAG 734 CTAG MaeIII 55 GTCAC ^GT[ACGT]AC 589 GTGAC MboI 740 GATC ^GATC 748 GATC MseI 326 TTAA T^TAA 503 TTAA MspA1I 100 CAGCTG C[AC]G^C[GT]G 398 CAGCTG NdeII 740 GATC ^GATC 748 GATC NlaIV 200 GGCTCC GG[ACGT]^[ACGT]CC 637 GGCTCC PvuII 100 CAGCTG CAG^CTG 398 CAGCTG Sau3AI 740 GATC ^GATC 748 GATC Sau96I 132 GGGCC G^G[ACGT]CC 170 GGCCC SfaNI 127 GATGC GCATC(5/9) 442 GATGC TaqI 723 TCGA T^CGA 750 TCGA Tru9I 326 TTAA T^TAA 503 TTAA TseI 109 GCAGC G^C[AT]GC 397 GCAGC Tsp45I 55 GTCAC ^GT[GC]AC 589 GTGAC TthHB8I 723 TCGA T^CGA 750 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cac8I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HinfI ItaI MboII MnlI MvaI MwoI ScrFI StyI TfiI Tsp509I