##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoRV       FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp45I      TspRI       
Tth111I     Van91I      XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AocI          232  CCTAAGG              CC^T[ACGT]AGG
AseI           16  ATTAAT               AT^TAAT
AsnI           16  ATTAAT               AT^TAAT
BamHI         141  GGATCC               G^GATCC
BbvI          337  GCAGC                GCAGC(8/12)
BfaI          116  CTAG                 C^TAG
BsaJI         219  CCAAGG               C^C[ACGT][ACGT]GG
BsgI          335  GTGCAG               GTGCAG(16/14)
BspMI           4  GCAGGT               ACCTGC(4/8)
BssSI          43  CACGAG               CACGAG(-5/-1)
BstYI         141  GGATCC               [GA]^GATC[CT]
Bsu36I        232  CCTAAGG              CC^T[ACGT]AGG
DdeI          233  CTAAG                C^T[ACGT]AG
DpnII         142  GATC                 ^GATC
DrdI           99  GACCATGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        232  CCTAAGG              CC^T[ACGT]AGG
EcoRI          66  GAATTC               G^AATTC
EcoT14I       219  CCAAGG               C^C[AT][AT]GG
EcoT22I       386  ATGCAT               ATGCA^T
Fnu4HI        337  GCAGC                GC^[ACGT]GC
HphI            7  GGTGA                GGTGA(8/7)
ItaI          337  GCAGC                GC^[ACGT]GC
MaeI          116  CTAG                 C^TAG
MboI          142  GATC                 ^GATC
MflI          141  GGATCC               [GA]^GATC[CT]
NdeII         142  GATC                 ^GATC
NlaIII        102  CATG                 CATG^
NsiI          386  ATGCAT               ATGCA^T
PleI          287  GAGTC                GAGTC(4/5)
PshBI          16  ATTAAT               AT^TAAT
Sau3AI        142  GATC                 ^GATC
StyI          219  CCAAGG               C^C[AT][AT]GG
TaqI          379  TCGA                 T^CGA
TfiI          266  GAATC                G^A[AT]TC
TseI          337  GCAGC                G^C[AT]GC
TthHB8I       379  TCGA                 T^CGA
XbaI          115  TCTAGA               T^CTAGA
XcmI           71  CCATTGGTGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         141  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          323  AGCT                 AG^CT
              339  AGCT                
AlwI          142  GATCC                GGATC(4/5)
              141  GGATC               
BbsI           22  GAAGAC               GAAGAC(2/6)
               96  GAAGAC              
BpuAI          22  GAAGAC               GAAGAC(2/6)
               96  GAAGAC              
BsrI           31  CCAGT                ACTGG(1/-1)
              244  CCAGT               
BstF5I        241  CATCC                GGATG(2/0)
               84  GGATG               
Cac8I         316  GCTGGC               GC[ACGT]^[ACGT]GC
              320  GCAAGC              
FokI          241  CATCC                GGATG(9/13)
               84  GGATG               
HinfI         266  GAATC                G^A[ACGT]TC
              287  GAGTC               
MnlI          363  CCTC                 CCTC(7/6)
              332  GAGG                
NlaIV         141  GGATCC               GG[ACGT]^[ACGT]CC
              369  GGAACC              
SfaNI          90  GATGC                GCATC(5/9)
              388  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AcsI        ApoI        MboII       MseI        Tru9I       
Tsp509I