################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII AccI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AviII AvrII BamHI BbsI BcgI BciVI BlnI BlpI Bpu1102I BpuAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspHI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KspI MamI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmeI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### Acc65I 1283 GGTACC G^GTACC AccIII 1082 TCCGGA T^CCGGA AclI 1213 AACGTT AA^CGTT AcsI 1179 AAATTT [GA]^AATT[CT] AflII 478 CTTAAG C^TTAAG AlwNI 672 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 541 CCTGAGG CC^T[ACGT]AGG ApoI 1179 AAATTT [GA]^AATT[CT] AseI 766 ATTAAT AT^TAAT AsnI 766 ATTAAT AT^TAAT Asp718I 1283 GGTACC G^GTACC AspHI 691 GAGCAC G[AT]GC[AT]^C BalI 232 TGGCCA TGG^CCA BbeI 112 GGCGCC GGCGC^C BbvCI 1590 GCTGAGG CCTCAGC(-5/-2) BclI 422 TGATCA T^GATCA BfrI 478 CTTAAG C^TTAAG BglI 442 GCCAGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1010 AGATCT A^GATCT BsaI 205 GAGACC GGTCTC(1/5) BsaWI 1082 TCCGGA [AT]^CCGG[AT] BseAI 1082 TCCGGA T^CCGGA BseRI 995 GAGGAG GAGGAG(10/8) BsgI 1059 GTGCAG GTGCAG(16/14) BsiHKAI 691 GAGCAC G[AT]GC[AT]^C BsmAI 205 GAGAC GTCTC(1/5) BspDI 757 ATCGAT AT^CGAT BspEI 1082 TCCGGA T^CCGGA BspLU11I 65 ACATGT A^CATGT BspMI 1703 ACCTGC ACCTGC(4/8) BstAPI 1607 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 335 TTCGAA TT^CGAA BstXI 410 CCACCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 541 CCTGAGG CC^T[ACGT]AGG ClaI 757 ATCGAT AT^CGAT DsaI 464 CCGTGG C^C[GA][CT]GG EarI 184 GAAGAG CTCTTC(1/4) Eco81I 541 CCTGAGG CC^T[ACGT]AGG EcoT14I 650 CCAAGG C^C[AT][AT]GG FbaI 422 TGATCA T^GATCA HgaI 855 GACGC GACGC(5/10) HinfI 735 GACTC G^A[ACGT]TC KasI 112 GGCGCC G^GCGCC KpnI 1283 GGTACC GGTAC^C Ksp632I 184 GAAGAG CTCTTC(1/4) MluNI 232 TGGCCA TGG^CCA MroI 1082 TCCGGA T^CCGGA MscI 232 TGGCCA TGG^CCA NarI 112 GGCGCC GG^CGCC NspI 65 ACATGT [GA]CATG^[CT] NspV 335 TTCGAA TT^CGAA PleI 735 GACTC GAGTC(4/5) PshBI 766 ATTAAT AT^TAAT Psp1406I 1213 AACGTT AA^CGTT PvuII 1263 CAGCTG CAG^CTG SexAI 314 ACCAGGT A^CC[AT]GGT SfoI 112 GGCGCC GGC^GCC SfuI 335 TTCGAA TT^CGAA SmaI 1107 CCCGGG CCC^GGG SmlI 478 CTTAAG C^T[CT][GA]AG SspI 1228 AATATT AAT^ATT StuI 606 AGGCCT AGG^CCT StyI 650 CCAAGG C^C[AT][AT]GG XmaCI 1107 CCCGGG C^CCGGG XmaI 1107 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 112 GGCGCC G[GA]^CG[CT]C 855 GACGCC AlwI 538 GATCC GGATC(4/5) 399 GGATC BbrPI 1075 CACGTG CAC^GTG 1597 CACGTG BfaI 1006 CTAG C^TAG 1208 CTAG BsaAI 1075 CACGTG [CT]AC^GT[GA] 1597 CACGTG BsaHI 112 GGCGCC G[GA]^CG[CT]C 855 GACGCC BsrBI 276 CCGCTC CCGCTC(-3/-3) 1638 GAGCGG BsrDI 176 GCAATG GCAATG(2/0) 533 GCAATG BsrFI 115 GCCGGC [GA]^CCGG[CT] 405 GCCGGC BstYI 399 GGATCT [GA]^GATC[CT] 1010 AGATCT Cfr10I 115 GCCGGC [GA]^CCGG[CT] 405 GCCGGC HaeII 8 GGCGCT [GA]GCGC^[CT] 112 GGCGCC Hin1I 112 GGCGCC G[GA]^CG[CT]C 855 GACGCC HindIII 251 AAGCTT A^AGCTT 331 AAGCTT MaeI 1006 CTAG C^TAG 1208 CTAG MflI 399 GGATCT [GA]^GATC[CT] 1010 AGATCT MspA1I 379 CAGCGG C[AC]G^C[GT]G 1263 CAGCTG NaeI 115 GCCGGC GCC^GGC 405 GCCGGC NgoMIV 115 GCCGGC G^CCGGC 405 GCCGGC PmaCI 1075 CACGTG CAC^GTG 1597 CACGTG PmlI 1075 CACGTG CAC^GTG 1597 CACGTG PstI 1528 CTGCAG CTGCA^G 1705 CTGCAG XhoII 399 GGATCT [GA]^GATC[CT] 1010 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI PshAI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I