(Circular) (Six-base) MAPSORT of: pFN21AE1992.seq Check: 5920 from: 1 to: 6418 pFN21AE1992 6418 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4228 278 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3761 1937 Size: 1824 4594 Acc65I G'GTAC_C Cuts at: 3288 3739 3288 Size: 451 5967 AclI AA'CG_TT Cuts at: 1563 3219 4545 4918 1563 Size: 1656 1326 373 3063 Fragments arranged by size: 3063 1656 1326 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4481 5865 6133 1632 Size: 2849 1384 268 1917 Fragments arranged by size: 2849 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2483 820 Size: 197 1466 4755 Fragments arranged by size: 4755 1466 197 AflIII A'CryG_T Cuts at: 1203 2070 3079 3081 3105 5317 6294 1203 Size: 867 1009 2 24 2212 977 1327 Fragments arranged by size: 2212 1327 1009 977 867 24 2 AgeI A'CCGG_T Cuts at: 5249 5249 Size: 6418 AhdI GACnn_n'nnGTC Cuts at: 5147 5147 Size: 6418 AleI CACnn'nnGTG Cuts at: 1166 2420 1166 Size: 1254 5164 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6418 AlwNI CAG_nnn'CTG Cuts at: 2682 4161 5733 2682 Size: 1479 1572 3367 Fragments arranged by size: 3367 1572 1479 ApaLI G'TGCA_C Cuts at: 4475 5631 4475 Size: 1156 5262 ApoI r'AATT_y Cuts at: 1493 3184 3727 3953 4105 6214 1493 Size: 1691 543 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 2772 4972 160 Size: 872 1740 2200 1606 Fragments arranged by size: 2200 1740 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2742 3112 3743 1870 Size: 73 134 665 370 631 4545 Fragments arranged by size: 4545 665 631 370 134 73 BamHI G'GATC_C Cuts at: 3748 4115 3748 Size: 367 6051 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 3288 3511 3739 5194 Size: 325 217 627 330 1171 223 228 1455 Cuts at: 5194 618 Size: 1842 Fragments arranged by size: 1842 1455 1171 627 330 325 228 223 217 BanII G_rGCy'C Cuts at: 1391 2560 2712 3521 3737 1391 Size: 1169 152 809 216 4072 Fragments arranged by size: 4072 1169 809 216 152 BbeI G_GCGC'C Cuts at: 2121 2121 Size: 6418 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5447 BbvCI CC'TCA_GC Cuts at: 3596 3596 Size: 6418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4609 4643 1903 Size: 34 50 34 2588 34 3678 Fragments arranged by size: 3678 2588 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4310 5520 4310 Size: 1210 5208 BclI T'GATC_A Cuts at: 1548 2427 1548 Size: 879 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2453 5029 136 Size: 107 122 71 631 605 781 2576 1525 Fragments arranged by size: 2576 1525 781 631 605 122 107 71 BglII A'GATC_T Cuts at: 3015 6413 3015 Size: 3398 3020 BlpI GC'TnA_GC Cuts at: 3835 3835 Size: 6418 Bme1580I G_kGCm'C Cuts at: 1790 3514 4479 5635 1790 Size: 1724 965 1156 2573 Fragments arranged by size: 2573 1724 1156 965 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6418 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2888 2975 4149 Size: 416 53 83 399 24 1471 87 1174 Cuts at: 4149 5107 442 Size: 958 1753 Fragments arranged by size: 1753 1471 1174 958 416 399 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6418 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2630 3604 2630 Size: 974 5444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2205 2726 3071 5078 1239 Size: 966 521 345 2007 2579 Fragments arranged by size: 2579 2007 966 521 345 Bpu10I CC'TnA_GC Cuts at: 2865 3254 3596 3653 2865 Size: 389 342 57 5630 Fragments arranged by size: 5630 389 342 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4543 5408 5706 4543 Size: 865 298 5255 Fragments arranged by size: 5255 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2204 5081 882 Size: 617 705 2877 2219 Fragments arranged by size: 2877 2219 705 617 BsaAI yAC'GTr Cuts at: 493 1204 3082 3604 4209 493 Size: 711 1878 522 605 2702 Fragments arranged by size: 2702 1878 711 605 522 BsaBI GATnn'nnATC Cuts at: 6412 6412 Size: 6418 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2118 2861 4225 4607 Size: 53 83 186 936 585 743 1364 382 Cuts at: 4607 5242 275 Size: 635 1451 Fragments arranged by size: 1451 1364 936 743 635 585 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3087 4851 5249 5523 5670 6081 3087 Size: 1764 398 274 147 411 3424 Fragments arranged by size: 3424 1764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2203 2233 1178 Size: 30 165 30 800 30 5363 Fragments arranged by size: 5363 800 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3015 1186 Size: 1829 4589 BseYI C'CCAG_C Cuts at: 2053 3431 5621 6117 2053 Size: 1378 2190 496 2354 Fragments arranged by size: 2354 2190 1378 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3085 1163 Size: 573 1349 4496 Fragments arranged by size: 4496 1349 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3868 4629 5657 1540 Size: 275 186 1867 761 1028 2301 Fragments arranged by size: 2301 1867 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2700 4479 4564 5635 2700 Size: 1779 85 1071 3483 Fragments arranged by size: 3483 1779 1071 85 BsmI GAATG_Cn' Cuts at: 3929 4022 3929 Size: 93 6325 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2560 2700 2712 3514 3521 3737 4479 Size: 399 770 140 12 802 7 216 742 Cuts at: 4479 4564 5635 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 802 770 742 399 216 140 85 12 7 BspEI T'CCGG_A Cuts at: 3087 6081 3087 Size: 2994 3424 BspHI T'CATG_A Cuts at: 4307 6037 4307 Size: 1730 4688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3706 3717 3773 844 Size: 2862 11 56 3489 Fragments arranged by size: 3489 2862 56 11 BsrBI CCG'CTC Cuts at: 1394 1823 2283 3645 4305 1394 Size: 429 460 1362 660 3507 Fragments arranged by size: 3507 1362 660 460 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2188 2545 4913 5087 65 Size: 1183 940 357 2368 174 1396 Fragments arranged by size: 2368 1396 1183 940 357 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 2410 5062 5249 1700 Size: 110 310 290 2652 187 2869 Fragments arranged by size: 2869 2652 310 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6418 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6418 BssSI C'ACGA_G Cuts at: 4478 5490 4478 Size: 1012 5406 BstAPI GCAn_nnn'nTGC Cuts at: 794 3618 794 Size: 2824 3594 BstBI TT'CG_AA Cuts at: 2341 5222 2341 Size: 2881 3537 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6418 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2422 Size: 6418 BstYI r'GATC_y Cuts at: 1972 2404 3015 3748 4115 4500 4517 5969 6055 Size: 432 611 733 367 385 17 1452 86 Cuts at: 6055 6067 6413 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 733 611 432 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2547 2547 Size: 6418 BtgI C'CryG_G Cuts at: 513 1065 2469 513 Size: 552 1404 4462 Fragments arranged by size: 4462 1404 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2044 487 Size: 683 56 818 4861 Fragments arranged by size: 4861 818 683 56 BtsI GCAGTG_nn' Cuts at: 2399 2680 3041 3127 3207 3695 3934 4728 4748 Size: 281 361 86 80 488 239 794 20 Cuts at: 4748 2399 Size: 4069 Fragments arranged by size: 4069 794 488 361 281 239 86 80 20 ClaI AT'CG_AT Cuts at: 1554 2763 1554 Size: 1209 5209 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6383 DraI TTT'AAA Cuts at: 3722 3722 Size: 6418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5425 809 Size: 507 4109 1802 Fragments arranged by size: 4109 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2237 2412 Size: 54 1425 50 275 18 267 140 175 Cuts at: 2412 3636 3865 4754 6383 8 Size: 1224 229 889 1629 43 Fragments arranged by size: 1629 1425 1224 889 275 267 229 175 140 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3865 1537 Size: 275 2053 4090 Fragments arranged by size: 4090 2053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 4348 1353 Size: 831 2164 3423 Fragments arranged by size: 3423 2164 831 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2744 5005 5379 5525 387 Size: 1064 138 1155 2261 374 146 1280 Fragments arranged by size: 2261 1280 1155 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2205 2726 3071 4481 5078 5865 6133 Size: 393 573 521 345 1410 597 787 268 Cuts at: 6133 1239 Size: 1524 Fragments arranged by size: 1524 1410 787 597 573 521 393 345 268 EcoNI CCTnn'n_nnAGG Cuts at: 3713 3713 Size: 6418 EcoO109I rG'GnC_Cy Cuts at: 2293 2659 2877 3348 3649 2293 Size: 366 218 471 301 5062 Fragments arranged by size: 5062 471 366 301 218 EcoRI G'AATT_C Cuts at: 3727 4105 3727 Size: 378 6040 FspI TGC'GCA Cuts at: 4924 4924 Size: 6418 HaeII r_GCGC'y Cuts at: 1678 2017 2121 5565 1678 Size: 339 104 3444 2531 Fragments arranged by size: 3444 2531 339 104 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2203 2235 5059 5091 5133 Size: 32 135 32 631 32 2824 32 42 Cuts at: 5133 5165 1373 Size: 32 2626 Fragments arranged by size: 2824 2626 631 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3762 4008 669 Size: 988 281 1824 246 3079 Fragments arranged by size: 3079 1824 988 281 246 HindIII A'AGCT_T Cuts at: 748 2256 2336 748 Size: 1508 80 4830 Fragments arranged by size: 4830 1508 80 HpaI GTT'AAC Cuts at: 4008 4008 Size: 6418 KasI G'GCGC_C Cuts at: 2117 2117 Size: 6418 KpnI G_GTAC'C Cuts at: 3292 3743 3292 Size: 451 5967 MfeI C'AATT_G Cuts at: 4017 4017 Size: 6418 MluI A'CGCG_T Cuts at: 6294 6294 Size: 6418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2245 3388 5532 5716 1305 Size: 940 1143 2144 184 2007 Fragments arranged by size: 2144 2007 1143 940 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2239 10 Size: 54 1425 343 407 4189 Fragments arranged by size: 4189 1425 407 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2420 2522 2774 2984 4376 4735 Size: 648 1015 239 102 252 210 1392 359 Cuts at: 4735 4894 518 Size: 159 2042 Fragments arranged by size: 2042 1392 1015 648 359 252 239 210 159 102 MspA1I CmG'CkG Cuts at: 1606 2386 3270 3834 4511 5659 5904 1606 Size: 780 884 564 677 1148 245 2120 Fragments arranged by size: 2120 1148 884 780 677 564 245 NaeI GCC'GGC Cuts at: 2122 2412 2122 Size: 290 6128 NarI GG'CG_CC Cuts at: 2118 2118 Size: 6418 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5866 NdeI CA'TA_TG Cuts at: 387 387 Size: 6418 NgoMIV G'CCGG_C Cuts at: 2120 2410 2120 Size: 290 6128 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6418 NotI GC'GGCC_GC Cuts at: 3865 3865 Size: 6418 NspI r_CATG'y Cuts at: 1132 1229 2074 3776 5321 1132 Size: 97 845 1702 1545 2229 Fragments arranged by size: 2229 1702 1545 845 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6418 PciI A'CATG_T Cuts at: 2070 5317 2070 Size: 3247 3171 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6418 PfoI T'CCnGG_A Cuts at: 2670 2715 2670 Size: 45 6373 PmeI GTTT'AAAC Cuts at: 3722 3722 Size: 6418 PmlI CAC'GTG Cuts at: 3082 3604 3082 Size: 522 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3005 3037 4487 4519 6000 6032 3005 Size: 32 1450 32 1481 32 3391 Fragments arranged by size: 3391 1481 1450 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2659 2877 2293 Size: 366 218 5834 Fragments arranged by size: 5834 366 218 PshAI GACnn'nnGTC Cuts at: 1613 2276 2379 2471 1613 Size: 663 103 92 5560 Fragments arranged by size: 5560 663 103 92 PsiI TTA'TAA Cuts at: 3527 3988 3527 Size: 461 5957 PstI C_TGCA'G Cuts at: 830 3537 3714 3770 830 Size: 2707 177 56 3478 Fragments arranged by size: 3478 2707 177 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6418 PvuII CAG'CTG Cuts at: 3270 3270 Size: 6418 SalI G'TCGA_C Cuts at: 1936 3760 1936 Size: 1824 4594 SbfI CC_TGCA'GG Cuts at: 3770 3770 Size: 6418 ScaI AGT'ACT Cuts at: 4666 6378 4666 Size: 1712 4706 SexAI A'CCwGG_T Cuts at: 2319 2319 Size: 6418 SfcI C'TryA_G Cuts at: 826 1045 2533 3533 3710 3766 4901 5582 5773 Size: 219 1488 1000 177 56 1135 681 191 Cuts at: 5773 826 Size: 1471 Fragments arranged by size: 1488 1471 1135 1000 681 219 191 177 56 SfoI GGC'GCC Cuts at: 2119 2119 Size: 6418 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6418 SmaI CCC'GGG Cuts at: 1872 3114 3745 1872 Size: 1242 631 4545 Fragments arranged by size: 4545 1242 631 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 4522 5423 5685 820 Size: 197 926 540 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 540 262 197 SnaBI TAC'GTA Cuts at: 493 4209 493 Size: 3716 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6418 SphI G_CATG'C Cuts at: 1132 3776 1132 Size: 2644 3774 SspI AAT'ATT Cuts at: 5 52 3235 4342 5 Size: 47 3183 1107 2081 Fragments arranged by size: 3183 2081 1107 47 StuI AGG'CCT Cuts at: 1517 2613 1517 Size: 1096 5322 StyI C'CwwG_G Cuts at: 513 1065 2655 3857 513 Size: 552 1590 1202 3074 Fragments arranged by size: 3074 1590 1202 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2770 4445 4462 4615 4800 5245 Size: 1255 458 732 1675 17 153 185 445 Cuts at: 5245 325 Size: 1498 Fragments arranged by size: 1675 1498 1255 732 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2073 4664 6376 Size: 274 80 33 51 1443 96 2591 1712 Cuts at: 6376 96 Size: 138 Fragments arranged by size: 2591 1712 1443 274 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6378 6410 6378 Size: 32 6386 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6418 XbaI T'CTAG_A Cuts at: 3754 3754 Size: 6418 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6418 XmaI C'CCGG_G Cuts at: 1870 3112 3743 1870 Size: 1242 631 4545 Fragments arranged by size: 4545 1242 631 XmnI GAAnn'nnTTC Cuts at: 4547 4547 Size: 6418 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4226 276 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI