(Linear) (Six-base) MAPSORT of: KIEE1992.seq Check: 7620 from: 1 to: 1713 KIEE1992.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. Acc65I G'GTAC_C Cuts at: 0 1283 1713 Size: 1283 430 AclI AA'CG_TT Cuts at: 0 1214 1713 Size: 1214 499 AflII C'TTAA_G Cuts at: 0 478 1713 Size: 478 1235 AflIII A'CryG_T Cuts at: 0 65 1074 1076 1100 1713 Size: 65 1009 2 24 613 Fragments arranged by size: 1009 613 65 24 2 AleI CACnn'nnGTG Cuts at: 0 415 1713 Size: 415 1298 AlwNI CAG_nnn'CTG Cuts at: 0 677 1713 Size: 677 1036 ApoI r'AATT_y Cuts at: 0 1179 1713 Size: 1179 534 AseI AT'TA_AT Cuts at: 0 767 1713 Size: 767 946 AvaI C'yCGr_G Cuts at: 0 72 737 1107 1713 Size: 72 665 370 606 Fragments arranged by size: 665 606 370 72 BanI G'GyrC_C Cuts at: 0 112 1283 1506 1713 Size: 112 1171 223 207 Fragments arranged by size: 1171 223 207 112 BanII G_rGCy'C Cuts at: 0 555 707 1516 1713 Size: 555 152 809 197 Fragments arranged by size: 809 555 197 152 BbeI G_GCGC'C Cuts at: 0 116 1713 Size: 116 1597 BbvCI CC'TCA_GC Cuts at: 0 1591 1713 Size: 1591 122 BclI T'GATC_A Cuts at: 0 422 1713 Size: 422 1291 BglI GCCn_nnn'nGGC Cuts at: 0 448 1713 Size: 448 1265 BglII A'GATC_T Cuts at: 0 1010 1713 Size: 1010 703 Bme1580I G_kGCm'C Cuts at: 0 1509 1713 Size: 1509 204 BmrI ACTGGGnnnn_n' Cuts at: 0 883 970 1713 Size: 883 87 743 Fragments arranged by size: 883 743 87 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 625 1599 1713 Size: 625 974 114 Fragments arranged by size: 974 625 114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 721 1066 1713 Size: 200 521 345 647 Fragments arranged by size: 647 521 345 200 Bpu10I CC'TnA_GC Cuts at: 0 860 1249 1591 1648 1713 Size: 860 389 342 57 65 Fragments arranged by size: 860 389 342 65 57 BsaI GGTCTCn'nnnn_ Cuts at: 0 199 1713 Size: 199 1514 BsaAI yAC'GTr Cuts at: 0 1077 1599 1713 Size: 1077 522 114 Fragments arranged by size: 1077 522 114 BsaHI Gr'CG_yC Cuts at: 0 113 856 1713 Size: 113 743 857 Fragments arranged by size: 857 743 113 BsaWI w'CCGG_w Cuts at: 0 1082 1713 Size: 1082 631 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 198 228 1713 Size: 198 30 1485 Fragments arranged by size: 1485 198 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1010 1713 Size: 1010 703 BseYI C'CCAG_C Cuts at: 0 48 1426 1713 Size: 48 1378 287 Fragments arranged by size: 1378 287 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1080 1713 Size: 1080 633 BsiHKAI G_wGCw'C Cuts at: 0 695 1713 Size: 695 1018 Bsp1286I G_dGCh'C Cuts at: 0 555 695 707 1509 1516 1713 Size: 555 140 12 802 7 197 Fragments arranged by size: 802 555 197 140 12 7 BspEI T'CCGG_A Cuts at: 0 1082 1713 Size: 1082 631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1712 1713 Size: 1712 1 BsrBI CCG'CTC Cuts at: 0 278 1640 1713 Size: 278 1362 73 Fragments arranged by size: 1362 278 73 BsrDI GCAATG_nn' Cuts at: 0 183 540 1713 Size: 183 357 1173 Fragments arranged by size: 1173 357 183 BsrFI r'CCGG_y Cuts at: 0 115 405 1713 Size: 115 290 1308 Fragments arranged by size: 1308 290 115 BstAPI GCAn_nnn'nTGC Cuts at: 0 1613 1713 Size: 1613 100 BstBI TT'CG_AA Cuts at: 0 336 1713 Size: 336 1377 BstXI CCAn_nnnn'nTGG Cuts at: 0 417 1713 Size: 417 1296 BstYI r'GATC_y Cuts at: 0 399 1010 1713 Size: 399 611 703 Fragments arranged by size: 703 611 399 Bsu36I CC'TnA_GG Cuts at: 0 542 1713 Size: 542 1171 BtgI C'CryG_G Cuts at: 0 464 1713 Size: 464 1249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 39 1713 Size: 39 1674 BtsI GCAGTG_nn' Cuts at: 0 394 675 1036 1122 1202 1690 1713 Size: 394 281 361 86 80 488 23 Fragments arranged by size: 488 394 361 281 86 80 23 ClaI AT'CG_AT Cuts at: 0 758 1713 Size: 758 955 EaeI y'GGCC_r Cuts at: 0 92 232 407 1631 1713 Size: 92 140 175 1224 82 Fragments arranged by size: 1224 175 140 92 82 EarI CTCTTCn'nnn_ Cuts at: 0 179 1713 Size: 179 1534 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 739 1713 Size: 739 974 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 721 1066 1713 Size: 200 521 345 647 Fragments arranged by size: 647 521 345 200 EcoO109I rG'GnC_Cy Cuts at: 0 288 654 872 1343 1644 1713 Size: 288 366 218 471 301 69 Fragments arranged by size: 471 366 301 288 218 69 HaeII r_GCGC'y Cuts at: 0 12 116 1713 Size: 12 104 1597 Fragments arranged by size: 1597 104 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 198 230 1713 Size: 198 32 1483 Fragments arranged by size: 1483 198 32 HindIII A'AGCT_T Cuts at: 0 251 331 1713 Size: 251 80 1382 Fragments arranged by size: 1382 251 80 KasI G'GCGC_C Cuts at: 0 112 1713 Size: 112 1601 KpnI G_GTAC'C Cuts at: 0 1287 1713 Size: 1287 426 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 240 1383 1713 Size: 240 1143 330 Fragments arranged by size: 1143 330 240 MscI TGG'CCA Cuts at: 0 234 1713 Size: 234 1479 MslI CAynn'nnrTG Cuts at: 0 176 415 517 769 979 1713 Size: 176 239 102 252 210 734 Fragments arranged by size: 734 252 239 210 176 102 MspA1I CmG'CkG Cuts at: 0 381 1265 1713 Size: 381 884 448 Fragments arranged by size: 884 448 381 NaeI GCC'GGC Cuts at: 0 117 407 1713 Size: 117 290 1306 Fragments arranged by size: 1306 290 117 NarI GG'CG_CC Cuts at: 0 113 1713 Size: 113 1600 NgoMIV G'CCGG_C Cuts at: 0 115 405 1713 Size: 115 290 1308 Fragments arranged by size: 1308 290 115 NspI r_CATG'y Cuts at: 0 69 1713 Size: 69 1644 PciI A'CATG_T Cuts at: 0 65 1713 Size: 65 1648 PfoI T'CCnGG_A Cuts at: 0 665 710 1713 Size: 665 45 1003 Fragments arranged by size: 1003 665 45 PmlI CAC'GTG Cuts at: 0 1077 1599 1713 Size: 1077 522 114 Fragments arranged by size: 1077 522 114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1000 1032 1713 Size: 1000 32 681 Fragments arranged by size: 1000 681 32 PpuMI rG'GwC_Cy Cuts at: 0 288 654 872 1713 Size: 288 366 218 841 Fragments arranged by size: 841 366 288 218 PshAI GACnn'nnGTC Cuts at: 0 271 374 466 1713 Size: 271 103 92 1247 Fragments arranged by size: 1247 271 103 92 PsiI TTA'TAA Cuts at: 0 1522 1713 Size: 1522 191 PstI C_TGCA'G Cuts at: 0 1532 1709 1713 Size: 1532 177 4 Fragments arranged by size: 1532 177 4 PvuII CAG'CTG Cuts at: 0 1265 1713 Size: 1265 448 SexAI A'CCwGG_T Cuts at: 0 314 1713 Size: 314 1399 SfcI C'TryA_G Cuts at: 0 528 1528 1705 1713 Size: 528 1000 177 8 Fragments arranged by size: 1000 528 177 8 SfoI GGC'GCC Cuts at: 0 114 1713 Size: 114 1599 SmaI CCC'GGG Cuts at: 0 1109 1713 Size: 1109 604 SmlI C'TyrA_G Cuts at: 0 478 1713 Size: 478 1235 SspI AAT'ATT Cuts at: 0 1230 1713 Size: 1230 483 StuI AGG'CCT Cuts at: 0 608 1713 Size: 608 1105 StyI C'CwwG_G Cuts at: 0 650 1713 Size: 650 1063 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 33 765 1713 Size: 33 732 948 Fragments arranged by size: 948 732 33 TatI w'GTAC_w Cuts at: 0 68 1713 Size: 68 1645 XmaI C'CCGG_G Cuts at: 0 1107 1713 Size: 1107 606 Enzymes that do cut: Acc65I AclI AflII AflIII AleI AlwNI ApoI AseI AvaI BanI BanII BbeI BbvCI BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmlI PpiI PpuMI PshAI PsiI PstI PvuII SexAI SfcI SfoI SmaI SmlI SspI StuI StyI TaqII TatI XmaI Enzymes that do not cut: AarI AatII AccI AcuI AfeI AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BlpI BmgBI BmtI BpuEI BsaBI BsiEI BsiWI BsmI BsmBI BspHI BsrGI BssHII BssSI BstEII BstZ17I CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI