################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI HphI KpnI MaeI MaeIII MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AcsI 562 GAATTC [GA]^AATT[CT] AcyI 570 GGCGCC G[GA]^CG[CT]C AgeI 166 ACCGGT A^CCGGT AlwI 261 GATCC GGATC(4/5) AlwNI 304 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 291 CCTCAGG CC^T[ACGT]AGG ApoI 562 GAATTC [GA]^AATT[CT] AvaI 325 CCCGAG C^[CT]CG[GA]G BanI 570 GGCGCC G^G[CT][GA]CC BbeI 570 GGCGCC GGCGC^C BbvCI 216 CCTCAGC CCTCAGC(-5/-2) BsaHI 570 GGCGCC G[GA]^CG[CT]C BsaWI 166 ACCGGT [AT]^CCGG[AT] BseRI 397 GAGGAG GAGGAG(10/8) BsmBI 79 GAGACG CGTCTC(1/5) BsoBI 325 CCCGAG C^[CT]CG[GA]G BsrDI 143 GCAATG GCAATG(2/0) BsrFI 166 ACCGGT [GA]^CCGG[CT] BsrI 420 CCAGT ACTGG(1/-1) BstYI 260 AGATCC [GA]^GATC[CT] Bsu36I 291 CCTCAGG CC^T[ACGT]AGG Cfr10I 166 ACCGGT [GA]^CCGG[CT] DpnII 261 GATC ^GATC DsaI 438 CCGCGG C^C[GA][CT]GG Eco81I 291 CCTCAGG CC^T[ACGT]AGG EcoRI 562 GAATTC G^AATTC HaeII 570 GGCGCC [GA]GCGC^[CT] Hin1I 570 GGCGCC G[GA]^CG[CT]C KasI 570 GGCGCC G^GCGCC KspI 438 CCGCGG CCGC^GG MaeII 515 ACGT A^CGT MboI 261 GATC ^GATC MflI 260 AGATCC [GA]^GATC[CT] MseI 257 TTAA T^TAA MslI 165 CACCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 570 GGCGCC GG^CGCC NdeII 261 GATC ^GATC NspI 554 GCATGC [GA]CATG^[CT] PinAI 166 ACCGGT A^CCGGT PpuMI 371 AGGTCCT [GA]G^G[AT]CC[CT] PstI 364 CTGCAG CTGCA^G SacII 438 CCGCGG CCGC^GG SapI 348 GCTCTTC GCTCTTC(1/4) Sau3AI 261 GATC ^GATC SfoI 570 GGCGCC GGC^GCC SgrAI 165 CACCGGTG C[GA]^CCGG[CT]G SphI 554 GCATGC GCATG^C Tru9I 257 TTAA T^TAA Tsp509I 563 AATT ^AATT XcmI 538 CCACTGCGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 260 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BalI 188 TGGCCA TGG^CCA 449 TGGCCA BanII 57 GAGCCC G[GA]GC[CT]^C 322 GAGCCC BmyI 57 GAGCCC G[AGT]GC[ACT]^C 322 GAGCCC BsgI 162 CTGCAC GTGCAG(16/14) 331 GTGCAG BsmAI 79 GAGAC GTCTC(1/5) 3 GTCTC BsmFI 524 GGGAC GGGAC(10/14) 342 GTCCC Bsp1286I 57 GAGCCC G[AGT]GC[ACT]^C 322 GAGCCC BssHII 467 GCGCGC G^CGCGC 469 GCGCGC DraII 32 AGGCCCC [GA]G^G[ACGT]CC[CT] 371 AGGTCCT EaeI 188 TGGCCA [CT]^GGCC[GA] 449 TGGCCA EarI 349 CTCTTC CTCTTC(1/4) 394 GAAGAG EcoO109I 32 AGGCCCC [GA]G^G[ACGT]CC[CT] 371 AGGTCCT HgaI 81 GACGC GACGC(5/10) 414 GACGC Ksp632I 349 CTCTTC CTCTTC(1/4) 394 GAAGAG MluNI 188 TGGCCA TGG^CCA 449 TGGCCA MscI 188 TGGCCA TGG^CCA 449 TGGCCA NlaIII 14 CATG CATG^ 555 CATG SfaNI 108 GATGC GCATC(5/9) 473 GCATC SfcI 177 CTACAG C^T[GA][CT]AG 364 CTGCAG TspRI 421 CAGTG CA[GC]TG[ACGT][ACGT]^ 539 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BcnI BpmI BsaJI BsiYI BslI BsrBI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PvuII Sau96I ScrFI TseI