(Circular) (Six-base) MAPSORT of: pFN21AE6575.seq Check: 2966 from: 1 to: 5290 pFN21AE6575 5290 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3100 278 Size: 53 83 186 936 1564 2468 Fragments arranged by size: 2468 1564 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2633 1937 Size: 696 4594 Acc65I G'GTAC_C Cuts at: 2611 2611 Size: 5290 AclI AA'CG_TT Cuts at: 1563 3417 3790 1563 Size: 1854 373 3063 Fragments arranged by size: 3063 1854 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3353 4737 5005 1632 Size: 1721 1384 268 1917 Fragments arranged by size: 1917 1721 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5093 AflIII A'CryG_T Cuts at: 1203 4189 5166 1203 Size: 2986 977 1327 Fragments arranged by size: 2986 1327 977 AgeI A'CCGG_T Cuts at: 2171 4121 2171 Size: 1950 3340 AhdI GACnn_n'nnGTC Cuts at: 4019 4019 Size: 5290 AleI CACnn'nnGTG Cuts at: 1166 2174 1166 Size: 1008 4282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5290 AlwNI CAG_nnn'CTG Cuts at: 2314 3033 4605 2314 Size: 719 1572 2999 Fragments arranged by size: 2999 1572 719 ApaLI G'TGCA_C Cuts at: 3347 4503 3347 Size: 1156 4134 ApoI r'AATT_y Cuts at: 1493 2567 2599 2825 2977 5086 1493 Size: 1074 32 226 152 2109 1697 Fragments arranged by size: 2109 1697 1074 226 152 32 AseI AT'TA_AT Cuts at: 160 1032 3844 160 Size: 872 2812 1606 Fragments arranged by size: 2812 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5290 AvaI C'yCGr_G Cuts at: 1870 1943 2330 2615 1870 Size: 73 387 285 4545 Fragments arranged by size: 4545 387 285 73 BamHI G'GATC_C Cuts at: 2620 2987 2620 Size: 367 4923 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2575 2611 4066 618 Size: 325 217 627 788 36 1455 1842 Fragments arranged by size: 1842 1455 788 627 325 217 36 BanII G_rGCy'C Cuts at: 1391 2066 2331 2609 1391 Size: 675 265 278 4072 Fragments arranged by size: 4072 675 278 265 BbeI G_GCGC'C Cuts at: 2579 2579 Size: 5290 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4319 BbvCI CC'TCA_GC Cuts at: 2222 2222 Size: 5290 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3481 3515 1903 Size: 34 1544 34 3678 Fragments arranged by size: 3678 1544 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3182 4392 3182 Size: 1210 4080 BclI T'GATC_A Cuts at: 1548 1548 Size: 5290 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3901 136 Size: 107 122 71 631 605 2229 1525 Fragments arranged by size: 2229 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5285 5285 Size: 5290 BlpI GC'TnA_GC Cuts at: 2707 2707 Size: 5290 Bme1580I G_kGCm'C Cuts at: 1790 3351 4507 1790 Size: 1561 1156 2573 Fragments arranged by size: 2573 1561 1156 BmgBI CAC'GTC Cuts at: 1318 2521 1318 Size: 1203 4087 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3021 3979 442 Size: 416 53 83 399 24 1604 958 1753 Fragments arranged by size: 1753 1604 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5290 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2402 2575 3950 1239 Size: 863 300 173 1375 2579 Fragments arranged by size: 2579 1375 863 300 173 Bpu10I CC'TnA_GC Cuts at: 2222 2222 Size: 5290 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3415 4280 4578 3415 Size: 865 298 4127 Fragments arranged by size: 4127 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3953 882 Size: 617 2454 2219 Fragments arranged by size: 2454 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3081 493 Size: 711 1877 2702 Fragments arranged by size: 2702 1877 711 BsaBI GATnn'nnATC Cuts at: 5284 5284 Size: 5290 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2576 3097 3479 4114 Size: 53 83 186 936 1043 521 382 635 Cuts at: 4114 275 Size: 1451 Fragments arranged by size: 1451 1043 936 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2171 3723 4121 4395 4542 4953 2171 Size: 1552 398 274 147 411 2508 Fragments arranged by size: 2508 1552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5065 Fragments arranged by size: 5065 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 1186 Size: 1231 4059 BseYI C'CCAG_C Cuts at: 2349 4493 4989 2349 Size: 2144 496 2650 Fragments arranged by size: 2650 2144 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2152 2357 1163 Size: 573 416 205 4096 Fragments arranged by size: 4096 573 416 205 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2740 3501 4529 1540 Size: 275 186 739 761 1028 2301 Fragments arranged by size: 2301 1028 761 739 275 186 BsiHKAI G_wGCw'C Cuts at: 3351 3436 4507 3351 Size: 85 1071 4134 Fragments arranged by size: 4134 1071 85 BsmI GAATG_Cn' Cuts at: 2801 2894 2801 Size: 93 5197 BsmBI CGTCTCn'nnnn_ Cuts at: 2078 2078 Size: 5290 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2331 2609 3351 3436 4507 1391 Size: 399 276 265 278 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 278 276 265 85 BspEI T'CCGG_A Cuts at: 4953 4953 Size: 5290 BspHI T'CATG_A Cuts at: 3179 4909 3179 Size: 1730 3560 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2645 844 Size: 1801 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2236 2361 2527 3177 1394 Size: 429 413 125 166 650 3507 Fragments arranged by size: 3507 650 429 413 166 125 BsrDI GCAATG_nn' Cuts at: 65 1248 2155 3785 3959 65 Size: 1183 907 1630 174 1396 Fragments arranged by size: 1630 1396 1183 907 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2171 3934 4121 1700 Size: 110 361 1763 187 2869 Fragments arranged by size: 2869 1763 361 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5290 BssHII G'CGCG_C Cuts at: 1925 2472 2474 1925 Size: 547 2 4741 Fragments arranged by size: 4741 547 2 BssSI C'ACGA_G Cuts at: 3350 4362 3350 Size: 1012 4278 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5290 BstBI TT'CG_AA Cuts at: 4094 4094 Size: 5290 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5290 BstYI r'GATC_y Cuts at: 1972 2265 2620 2987 3372 3389 4841 4927 4939 Size: 293 355 367 385 17 1452 86 12 Cuts at: 4939 5285 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 355 346 293 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2297 2297 Size: 5290 BtgI C'CryG_G Cuts at: 513 1065 2443 513 Size: 552 1378 3360 Fragments arranged by size: 3360 1378 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4551 Fragments arranged by size: 4551 683 56 BtsI GCAGTG_nn' Cuts at: 2543 2806 3600 3620 2543 Size: 263 794 20 4213 Fragments arranged by size: 4213 794 263 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5290 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5255 DraI TTT'AAA Cuts at: 2594 2594 Size: 5290 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4297 809 Size: 507 2981 1802 Fragments arranged by size: 2981 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 2454 2737 Size: 54 1425 50 275 18 363 261 283 Cuts at: 2737 3626 5255 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 363 283 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2737 1537 Size: 275 925 4090 Fragments arranged by size: 4090 925 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 2394 3220 1353 Size: 1007 34 826 3423 Fragments arranged by size: 3423 1007 826 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2333 3877 4251 4397 387 Size: 1064 138 744 1544 374 146 1280 Fragments arranged by size: 1544 1280 1064 744 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2402 2575 3353 3950 4737 5005 Size: 393 470 300 173 778 597 787 268 Cuts at: 5005 1239 Size: 1524 Fragments arranged by size: 1524 787 778 597 470 393 300 268 173 EcoO109I rG'GnC_Cy Cuts at: 2038 2377 2038 Size: 339 4951 EcoRI G'AATT_C Cuts at: 2567 2599 2977 2567 Size: 32 378 4880 Fragments arranged by size: 4880 378 32 FspI TGC'GCA Cuts at: 3796 3796 Size: 5290 HaeII r_GCGC'y Cuts at: 1678 2579 4437 1678 Size: 901 1858 2531 Fragments arranged by size: 2531 1858 901 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 3931 3963 4005 Size: 32 135 32 455 32 1872 32 42 Cuts at: 4005 4037 1373 Size: 32 2626 Fragments arranged by size: 2626 1872 455 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2634 2880 669 Size: 988 281 696 246 3079 Fragments arranged by size: 3079 988 696 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5290 HpaI GTT'AAC Cuts at: 2880 2880 Size: 5290 KasI G'GCGC_C Cuts at: 2575 2575 Size: 5290 KpnI G_GTAC'C Cuts at: 2615 2615 Size: 5290 MfeI C'AATT_G Cuts at: 2889 2889 Size: 5290 MluI A'CGCG_T Cuts at: 5166 5166 Size: 5290 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4404 4588 1305 Size: 3099 184 2007 Fragments arranged by size: 3099 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 2456 10 Size: 54 1425 343 363 261 2844 Fragments arranged by size: 2844 1425 363 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2174 3248 3607 3766 518 Size: 648 1008 1074 359 159 2042 Fragments arranged by size: 2042 1074 1008 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2187 2245 2311 2445 2706 3383 4531 Size: 531 50 58 66 134 261 677 1148 Cuts at: 4531 4776 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 531 261 245 134 66 58 50 NarI GG'CG_CC Cuts at: 2576 2576 Size: 5290 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4738 NdeI CA'TA_TG Cuts at: 387 387 Size: 5290 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5290 NotI GC'GGCC_GC Cuts at: 2737 2737 Size: 5290 NspI r_CATG'y Cuts at: 1132 1229 2563 2648 4193 1132 Size: 97 1334 85 1545 2229 Fragments arranged by size: 2229 1545 1334 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5290 PciI A'CATG_T Cuts at: 4189 4189 Size: 5290 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5290 PfoI T'CCnGG_A Cuts at: 2379 2552 2379 Size: 173 5117 PmeI GTTT'AAAC Cuts at: 2594 2594 Size: 5290 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3359 3391 4872 4904 3359 Size: 32 1481 32 3745 Fragments arranged by size: 3745 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2377 2377 Size: 5290 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5290 PsiI TTA'TAA Cuts at: 2860 2860 Size: 5290 PstI C_TGCA'G Cuts at: 830 2373 2642 830 Size: 1543 269 3478 Fragments arranged by size: 3478 1543 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5290 PvuII CAG'CTG Cuts at: 2187 2245 2311 2187 Size: 58 66 5166 Fragments arranged by size: 5166 66 58 SacII CC_GC'GG Cuts at: 2446 2446 Size: 5290 SalI G'TCGA_C Cuts at: 1936 2632 1936 Size: 696 4594 SapI GCTCTTCn'nnn_ Cuts at: 2360 2360 Size: 5290 SbfI CC_TGCA'GG Cuts at: 2642 2642 Size: 5290 ScaI AGT'ACT Cuts at: 3538 5250 3538 Size: 1712 3578 SfcI C'TryA_G Cuts at: 826 1045 2182 2369 2638 3773 4454 4645 826 Size: 219 1137 187 269 1135 681 191 1471 Fragments arranged by size: 1471 1137 1135 681 269 219 191 187 SfoI GGC'GCC Cuts at: 2577 2577 Size: 5290 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5290 SgrAI Cr'CCGG_yG Cuts at: 1700 2171 1700 Size: 471 4819 SmaI CCC'GGG Cuts at: 1872 2617 1872 Size: 745 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3394 4295 4557 820 Size: 197 926 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3081 493 Size: 2588 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5290 SphI G_CATG'C Cuts at: 1132 2563 2648 1132 Size: 1431 85 3774 Fragments arranged by size: 3774 1431 85 SspI AAT'ATT Cuts at: 5 52 3214 5 Size: 47 3162 2081 Fragments arranged by size: 3162 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5290 StyI C'CwwG_G Cuts at: 513 1065 2729 513 Size: 552 1664 3074 Fragments arranged by size: 3074 1664 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2286 3317 3334 3487 3672 4117 325 Size: 1255 706 1031 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1031 706 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3536 5248 96 Size: 274 80 33 51 1443 1559 1712 138 Fragments arranged by size: 1712 1559 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5250 5282 5250 Size: 32 5258 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5290 XbaI T'CTAG_A Cuts at: 2626 2626 Size: 5290 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2550 2550 Size: 5290 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5290 XmaI C'CCGG_G Cuts at: 1870 2615 1870 Size: 745 4545 XmnI GAAnn'nnTTC Cuts at: 3419 3419 Size: 5290 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3098 276 Size: 53 83 186 936 1564 2468 Fragments arranged by size: 2468 1564 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI