(Linear) (Six-base) MAPSORT of: KIEE6575.seq Check: 5244 from: 1 to: 585 KIEE6575.orf With 182 enzymes: SgfI * March 16, 2015 11:33 .. AgeI A'CCGG_T Cuts at: 0 166 585 Size: 166 419 AleI CACnn'nnGTG Cuts at: 0 169 585 Size: 169 416 AlwNI CAG_nnn'CTG Cuts at: 0 309 585 Size: 309 276 ApoI r'AATT_y Cuts at: 0 562 585 Size: 562 23 AvaI C'yCGr_G Cuts at: 0 325 585 Size: 325 260 BanI G'GyrC_C Cuts at: 0 570 585 Size: 570 15 BanII G_rGCy'C Cuts at: 0 61 326 585 Size: 61 265 259 Fragments arranged by size: 265 259 61 BbeI G_GCGC'C Cuts at: 0 574 585 Size: 574 11 BbvCI CC'TCA_GC Cuts at: 0 217 585 Size: 217 368 BmgBI CAC'GTC Cuts at: 0 516 585 Size: 516 69 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 397 570 585 Size: 97 300 173 15 Fragments arranged by size: 300 173 97 15 Bpu10I CC'TnA_GC Cuts at: 0 217 585 Size: 217 368 BsaHI Gr'CG_yC Cuts at: 0 571 585 Size: 571 14 BsaWI w'CCGG_w Cuts at: 0 166 585 Size: 166 419 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 412 585 Size: 412 173 BseYI C'CCAG_C Cuts at: 0 344 585 Size: 344 241 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 147 352 585 Size: 147 205 233 Fragments arranged by size: 233 205 147 BsmBI CGTCTCn'nnnn_ Cuts at: 0 73 585 Size: 73 512 Bsp1286I G_dGCh'C Cuts at: 0 61 326 585 Size: 61 265 259 Fragments arranged by size: 265 259 61 BsrBI CCG'CTC Cuts at: 0 231 356 522 585 Size: 231 125 166 63 Fragments arranged by size: 231 166 125 63 BsrDI GCAATG_nn' Cuts at: 0 150 585 Size: 150 435 BsrFI r'CCGG_y Cuts at: 0 166 585 Size: 166 419 BssHII G'CGCG_C Cuts at: 0 467 469 585 Size: 467 2 116 Fragments arranged by size: 467 116 2 BstYI r'GATC_y Cuts at: 0 260 585 Size: 260 325 Bsu36I CC'TnA_GG Cuts at: 0 292 585 Size: 292 293 BtgI C'CryG_G Cuts at: 0 438 585 Size: 438 147 BtsI GCAGTG_nn' Cuts at: 0 538 585 Size: 538 47 EaeI y'GGCC_r Cuts at: 0 188 449 585 Size: 188 261 136 Fragments arranged by size: 261 188 136 EarI CTCTTCn'nnn_ Cuts at: 0 355 389 585 Size: 355 34 196 Fragments arranged by size: 355 196 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 328 585 Size: 328 257 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 397 570 585 Size: 97 300 173 15 Fragments arranged by size: 300 173 97 15 EcoO109I rG'GnC_Cy Cuts at: 0 33 372 585 Size: 33 339 213 Fragments arranged by size: 339 213 33 EcoRI G'AATT_C Cuts at: 0 562 585 Size: 562 23 HaeII r_GCGC'y Cuts at: 0 574 585 Size: 574 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 22 54 585 Size: 22 32 531 Fragments arranged by size: 531 32 22 KasI G'GCGC_C Cuts at: 0 570 585 Size: 570 15 MscI TGG'CCA Cuts at: 0 190 451 585 Size: 190 261 134 Fragments arranged by size: 261 190 134 MslI CAynn'nnrTG Cuts at: 0 169 585 Size: 169 416 MspA1I CmG'CkG Cuts at: 0 132 182 240 306 440 585 Size: 132 50 58 66 134 145 Fragments arranged by size: 145 134 132 66 58 50 NarI GG'CG_CC Cuts at: 0 571 585 Size: 571 14 NspI r_CATG'y Cuts at: 0 558 585 Size: 558 27 PfoI T'CCnGG_A Cuts at: 0 374 547 585 Size: 374 173 38 Fragments arranged by size: 374 173 38 PpuMI rG'GwC_Cy Cuts at: 0 372 585 Size: 372 213 PstI C_TGCA'G Cuts at: 0 368 585 Size: 368 217 PvuII CAG'CTG Cuts at: 0 182 240 306 585 Size: 182 58 66 279 Fragments arranged by size: 279 182 66 58 SacII CC_GC'GG Cuts at: 0 441 585 Size: 441 144 SapI GCTCTTCn'nnn_ Cuts at: 0 355 585 Size: 355 230 SfcI C'TryA_G Cuts at: 0 177 364 585 Size: 177 187 221 Fragments arranged by size: 221 187 177 SfoI GGC'GCC Cuts at: 0 572 585 Size: 572 13 SgrAI Cr'CCGG_yG Cuts at: 0 166 585 Size: 166 419 SphI G_CATG'C Cuts at: 0 558 585 Size: 558 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 281 585 Size: 281 304 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 545 585 Size: 545 40 Enzymes that do cut: AgeI AleI AlwNI ApoI AvaI BanI BanII BbeI BbvCI BmgBI BpmI Bpu10I BsaHI BsaWI BseRI BseYI BsgI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssHII BstYI Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I KasI MscI MslI MspA1I NarI NspI PfoI PpuMI PstI PvuII SacII SapI SfcI SfoI SgrAI SphI TaqII XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI SmaI SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI