################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsoBI BspDI BspEI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 487 GTAGAC GT^[AC][GT]AC AflIII 1572 ACATGT A^C[GA][CT]GT BalI 353 TGGCCA TGG^CCA BanI 1341 GGTGCC G^G[CT][GA]CC BbvCI 813 CCTCAGC CCTCAGC(-5/-2) BcgI 368 GCAGCACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1355 GGATAC GTATCC(6/5) BclI 1428 TGATCA T^GATCA BcnI 22 CCGGG CC^[GC]GG BsmBI 1294 CGTCTC CGTCTC(1/5) BsmI 848 GCATTC GAATGC(1/-1) Bsp1407I 380 TGTACA T^GTACA BspHI 1097 TCATGA T^CATGA BspLU11I 1572 ACATGT A^CATGT BspMI 1058 ACCTGC ACCTGC(4/8) BsrBI 716 CCGCTC CCGCTC(-3/-3) BsrGI 380 TGTACA T^GTACA BstAPI 1489 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1288 GGTTACC G^GT[ACGT]ACC BstPI 1288 GGTTACC G^GT[ACGT]ACC DraIII 427 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1425 GACTGATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 353 TGGCCA [CT]^GGCC[GA] EcoNI 1671 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1288 GGTTACC G^GT[ACGT]ACC EcoRI 1188 GAATTC G^AATTC EcoRV 1726 GATATC GAT^ATC FauI 715 CCCGC CCCGC(4/6) FbaI 1428 TGATCA T^GATCA HapII 22 CCGG C^CGG HindIII 645 AAGCTT A^AGCTT HpaII 22 CCGG C^CGG MfeI 1582 CAATTG C^AATTG MluNI 353 TGGCCA TGG^CCA MscI 353 TGGCCA TGG^CCA MspI 22 CCGG C^CGG MunI 1582 CAATTG C^AATTG NciI 22 CCGGG CC^[GC]GG NcoI 413 CCATGG C^CATGG NdeI 1719 CATATG CA^TATG NspI 1572 ACATGT [GA]CATG^[CT] PshAI 888 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1097 TCATGA T^CATGA SapI 760 GAAGAGC GCTCTTC(1/4) ScaI 1331 AGTACT AGT^ACT SmlI 1675 CTCAAG C^T[CT][GA]AG SspBI 380 TGTACA T^GTACA SspI 1382 AATATT AAT^ATT XbaI 870 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 716 CCGC CCGC(-3/-1) 971 CCGC AcsI 796 GAATTT [GA]^AATT[CT] 1188 GAATTC ApoI 796 GAATTT [GA]^AATT[CT] 1188 GAATTC BpmI 5 CTCCAG CTGGAG(16/14) 1042 CTCCAG BseRI 465 CTCCTC GAGGAG(10/8) 1534 GAGGAG BsgI 1060 CTGCAC GTGCAG(16/14) 1021 GTGCAG BsmFI 1067 GGGAC GGGAC(10/14) 1604 GGGAC DsaI 413 CCATGG C^C[GA][CT]GG 1031 CCACGG EarI 760 GAAGAG CTCTTC(1/4) 1557 GAAGAG Ksp632I 760 GAAGAG CTCTTC(1/4) 1557 GAAGAG MaeII 893 ACGT A^CGT 1470 ACGT PflMI 853 CCAGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1614 CCAGTTAATGG PstI 1312 CTGCAG CTGCA^G 1487 CTGCAG SexAI 471 ACCTGGT A^CC[AT]GGT 1337 ACCAGGT Van91I 853 CCAGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1614 CCAGTTAATGG XcmI 520 CCAGCATTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1394 CCAAGGAGGATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AfaI AluI AlwI AlwNI ApaI Asp700I AvaII BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI