(Circular) (Six-base) MAPSORT of: pFN21AE2205.seq Check: 318 from: 1 to: 6478 pFN21AE2205 6478 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4288 278 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2493 3821 1937 Size: 556 1328 4594 Fragments arranged by size: 4594 1328 556 Acc65I G'GTAC_C Cuts at: 3799 3799 Size: 6478 AclI AA'CG_TT Cuts at: 1563 4605 4978 1563 Size: 3042 373 3063 Fragments arranged by size: 3063 3042 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 3215 3347 3644 4541 5925 6193 1632 Size: 476 1107 132 297 897 1384 268 1917 Fragments arranged by size: 1917 1384 1107 897 476 297 268 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6281 AflIII A'CryG_T Cuts at: 1203 3577 5377 6354 1203 Size: 2374 1800 977 1327 Fragments arranged by size: 2374 1800 1327 977 AgeI A'CCGG_T Cuts at: 5309 5309 Size: 6478 AhdI GACnn_n'nnGTC Cuts at: 5207 5207 Size: 6478 AleI CACnn'nnGTG Cuts at: 1166 2381 1166 Size: 1215 5263 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6478 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3119 3151 3119 Size: 32 6446 AlwNI CAG_nnn'CTG Cuts at: 3055 3316 3325 3500 4221 5793 3055 Size: 261 9 175 721 1572 3740 Fragments arranged by size: 3740 1572 721 261 175 9 ApaI G_GGCC'C Cuts at: 2033 3157 3619 2033 Size: 1124 462 4892 Fragments arranged by size: 4892 1124 462 ApaLI G'TGCA_C Cuts at: 4535 5691 4535 Size: 1156 5322 ApoI r'AATT_y Cuts at: 1493 2801 3193 3787 4013 4165 6274 1493 Size: 1308 392 594 226 152 2109 1697 Fragments arranged by size: 2109 1697 1308 594 392 226 152 AseI AT'TA_AT Cuts at: 160 1032 5032 160 Size: 872 4000 1606 Fragments arranged by size: 4000 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 AvaI C'yCGr_G Cuts at: 1870 1943 3803 1870 Size: 73 1860 4545 Fragments arranged by size: 4545 1860 73 BamHI G'GATC_C Cuts at: 3808 4175 3808 Size: 367 6111 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3346 3799 5254 618 Size: 325 217 627 1559 453 1455 1842 Fragments arranged by size: 1842 1559 1455 627 453 325 217 BanII G_rGCy'C Cuts at: 1391 2033 3157 3619 3797 1391 Size: 642 1124 462 178 4072 Fragments arranged by size: 4072 1124 642 462 178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2701 3091 3647 928 Size: 971 802 390 556 3759 Fragments arranged by size: 3759 971 802 556 390 BbvCI CC'TCA_GC Cuts at: 2819 2819 Size: 6478 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2362 2396 4669 4703 1903 Size: 34 425 34 2273 34 3678 Fragments arranged by size: 3678 2273 425 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3354 4370 5580 3354 Size: 1016 1210 4252 Fragments arranged by size: 4252 1210 1016 BclI T'GATC_A Cuts at: 1548 3433 1548 Size: 1885 4593 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5089 136 Size: 107 122 71 631 605 3417 1525 Fragments arranged by size: 3417 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6473 6473 Size: 6478 BlpI GC'TnA_GC Cuts at: 3895 3895 Size: 6478 Bme1580I G_kGCm'C Cuts at: 1790 2033 3157 3619 4539 5695 1790 Size: 243 1124 462 920 1156 2573 Fragments arranged by size: 2573 1156 1124 920 462 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6478 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3151 3613 4209 Size: 416 53 83 399 24 1734 462 596 Cuts at: 4209 5167 442 Size: 958 1753 Fragments arranged by size: 1753 1734 958 596 462 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6478 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2356 2356 Size: 6478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1995 3032 5138 1239 Size: 756 1037 2106 2579 Fragments arranged by size: 2579 2106 1037 756 Bpu10I CC'TnA_GC Cuts at: 2369 2819 2369 Size: 450 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3665 4603 5468 5766 3665 Size: 938 865 298 4377 Fragments arranged by size: 4377 938 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5141 882 Size: 617 3642 2219 Fragments arranged by size: 3642 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4269 493 Size: 711 3065 2702 Fragments arranged by size: 3065 2702 711 BsaBI GATnn'nnATC Cuts at: 6472 6472 Size: 6478 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4285 4667 5302 275 Size: 53 83 186 936 2752 382 635 1451 Fragments arranged by size: 2752 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4911 5309 5583 5730 6141 4911 Size: 398 274 147 411 5248 Fragments arranged by size: 5248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6253 Fragments arranged by size: 6253 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2461 3554 1186 Size: 1275 1093 4110 Fragments arranged by size: 4110 1275 1093 BseYI C'CCAG_C Cuts at: 2180 5681 6177 2180 Size: 3501 496 2481 Fragments arranged by size: 3501 2481 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3047 3050 1163 Size: 573 1311 3 4591 Fragments arranged by size: 4591 1311 573 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3928 4689 5717 1540 Size: 275 186 1927 761 1028 2301 Fragments arranged by size: 2301 1927 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4539 4624 5695 4539 Size: 85 1071 5322 Fragments arranged by size: 5322 1071 85 BsmI GAATG_Cn' Cuts at: 2853 3989 4082 2853 Size: 1136 93 5249 Fragments arranged by size: 5249 1136 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3305 3305 Size: 6478 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 3157 3619 3797 4539 4624 5695 Size: 399 243 1124 462 178 742 85 1071 Cuts at: 5695 1391 Size: 2174 Fragments arranged by size: 2174 1124 1071 742 462 399 243 178 85 BspEI T'CCGG_A Cuts at: 6141 6141 Size: 6478 BspHI T'CATG_A Cuts at: 3102 4367 6097 3102 Size: 1265 1730 3483 Fragments arranged by size: 3483 1730 1265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3072 3833 844 Size: 2228 761 3489 Fragments arranged by size: 3489 2228 761 BsrBI CCG'CTC Cuts at: 1394 1823 2723 4365 1394 Size: 429 900 1642 3507 Fragments arranged by size: 3507 1642 900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4973 5147 65 Size: 1183 3725 174 1396 Fragments arranged by size: 3725 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5122 5309 1700 Size: 110 3312 187 2869 Fragments arranged by size: 3312 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2385 96 Size: 2289 4189 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6478 BssSI C'ACGA_G Cuts at: 4538 5550 4538 Size: 1012 5466 BstAPI GCAn_nnn'nTGC Cuts at: 794 3500 794 Size: 2706 3772 BstBI TT'CG_AA Cuts at: 5282 5282 Size: 6478 BstEII G'GTnAC_C Cuts at: 1184 3293 1184 Size: 2109 4369 BstYI r'GATC_y Cuts at: 1972 2091 2346 2920 3808 4175 4560 4577 6029 Size: 119 255 574 888 367 385 17 1452 Cuts at: 6029 6115 6127 6473 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 888 574 385 367 346 255 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 3036 513 Size: 552 939 414 618 3955 Fragments arranged by size: 3955 939 618 552 414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5739 Fragments arranged by size: 5739 683 56 BtsI GCAGTG_nn' Cuts at: 2586 2920 2985 3994 4788 4808 2586 Size: 334 65 1009 794 20 4256 Fragments arranged by size: 4256 1009 794 334 65 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6478 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6443 DraI TTT'AAA Cuts at: 3782 3782 Size: 6478 DraIII CAC_nnn'GTG Cuts at: 2437 2437 Size: 6478 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3436 5485 809 Size: 507 2120 2049 1802 Fragments arranged by size: 2120 2049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 3925 4814 Size: 54 1425 50 275 18 528 1567 889 Cuts at: 4814 6443 8 Size: 1629 43 Fragments arranged by size: 1629 1567 1425 889 528 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3925 1537 Size: 275 2113 4090 Fragments arranged by size: 4090 2113 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2760 3557 4408 1353 Size: 1407 797 851 3423 Fragments arranged by size: 3423 1407 851 797 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5065 5439 5585 387 Size: 1064 138 3476 374 146 1280 Fragments arranged by size: 3476 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2108 3032 3215 3347 3644 4541 Size: 393 363 113 924 183 132 297 897 Cuts at: 4541 5138 5925 6193 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 924 897 787 597 393 363 297 268 183 132 113 EcoNI CCTnn'n_nnAGG Cuts at: 3680 3680 Size: 6478 EcoO109I rG'GnC_Cy Cuts at: 2302 3153 3615 2302 Size: 851 462 5165 Fragments arranged by size: 5165 851 462 EcoRI G'AATT_C Cuts at: 3193 3787 4165 3193 Size: 594 378 5506 Fragments arranged by size: 5506 594 378 EcoRV GAT'ATC Cuts at: 3733 3733 Size: 6478 FspI TGC'GCA Cuts at: 4984 4984 Size: 6478 HaeII r_GCGC'y Cuts at: 1678 5625 1678 Size: 3947 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2568 2600 3634 3666 5119 Size: 32 135 32 996 32 1034 32 1453 Cuts at: 5119 5151 5193 5225 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1453 1034 996 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3822 4068 669 Size: 988 281 1884 246 3079 Fragments arranged by size: 3079 1884 988 281 246 HindIII A'AGCT_T Cuts at: 748 2650 748 Size: 1902 4576 HpaI GTT'AAC Cuts at: 4068 4068 Size: 6478 KpnI G_GTAC'C Cuts at: 3803 3803 Size: 6478 MfeI C'AATT_G Cuts at: 3587 4077 3587 Size: 490 5988 MluI A'CGCG_T Cuts at: 6354 6354 Size: 6478 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3141 3495 5592 5776 1305 Size: 1836 354 2097 184 2007 Fragments arranged by size: 2097 2007 1836 354 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2360 10 Size: 54 1425 343 528 4128 Fragments arranged by size: 4128 1425 528 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2381 2533 2829 4436 4795 4954 518 Size: 648 1215 152 296 1607 359 159 2042 Fragments arranged by size: 2042 1607 1215 648 359 296 159 152 MspA1I CmG'CkG Cuts at: 1606 2065 2504 3031 3894 4571 5719 5964 1606 Size: 459 439 527 863 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 677 527 459 439 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 4573 Fragments arranged by size: 4573 939 552 414 NdeI CA'TA_TG Cuts at: 387 3725 387 Size: 3338 3140 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6478 NotI GC'GGCC_GC Cuts at: 3925 3925 Size: 6478 NspI r_CATG'y Cuts at: 1132 1229 3581 3836 5381 1132 Size: 97 2352 255 1545 2229 Fragments arranged by size: 2352 2229 1545 255 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6478 PciI A'CATG_T Cuts at: 3577 5377 3577 Size: 1800 4678 PflMI CCAn_nnn'nTGG Cuts at: 1108 2864 3625 1108 Size: 1756 761 3961 Fragments arranged by size: 3961 1756 761 PfoI T'CCnGG_A Cuts at: 2342 3048 2342 Size: 706 5772 PmeI GTTT'AAAC Cuts at: 3782 3782 Size: 6478 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3663 3695 4547 4579 6060 6092 3663 Size: 32 852 32 1481 32 4049 Fragments arranged by size: 4049 1481 852 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2897 1613 Size: 1284 5194 PsiI TTA'TAA Cuts at: 4048 4048 Size: 6478 PspOMI G'GGCC_C Cuts at: 2029 3153 3615 2029 Size: 1124 462 4892 Fragments arranged by size: 4892 1124 462 PstI C_TGCA'G Cuts at: 830 3321 3496 3830 830 Size: 2491 175 334 3478 Fragments arranged by size: 3478 2491 334 175 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6478 PvuII CAG'CTG Cuts at: 2065 2504 3031 2065 Size: 439 527 5512 Fragments arranged by size: 5512 527 439 SalI G'TCGA_C Cuts at: 1936 3820 1936 Size: 1884 4594 SapI GCTCTTCn'nnn_ Cuts at: 2760 2760 Size: 6478 SbfI CC_TGCA'GG Cuts at: 3830 3830 Size: 6478 ScaI AGT'ACT Cuts at: 3338 4726 6438 3338 Size: 1388 1712 3378 Fragments arranged by size: 3378 1712 1388 SexAI A'CCwGG_T Cuts at: 2476 3342 2476 Size: 866 5612 SfcI C'TryA_G Cuts at: 826 1045 2490 3317 3425 3492 3826 4961 5642 Size: 219 1445 827 108 67 334 1135 681 Cuts at: 5642 5833 826 Size: 191 1471 Fragments arranged by size: 1471 1445 1135 827 681 334 219 191 108 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6478 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6478 SmaI CCC'GGG Cuts at: 1872 3805 1872 Size: 1933 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3680 4582 5483 5745 820 Size: 197 926 1737 902 901 262 1553 Fragments arranged by size: 1737 1553 926 902 901 262 197 SnaBI TAC'GTA Cuts at: 493 4269 493 Size: 3776 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6478 SphI G_CATG'C Cuts at: 1132 3836 1132 Size: 2704 3774 SspI AAT'ATT Cuts at: 5 52 3389 4402 5 Size: 47 3337 1013 2081 Fragments arranged by size: 3337 2081 1013 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6478 StyI C'CwwG_G Cuts at: 513 1065 2004 2229 2355 2418 2952 3350 3399 Size: 552 939 225 126 63 534 398 49 Cuts at: 3399 3917 513 Size: 518 3074 Fragments arranged by size: 3074 939 552 534 518 398 225 126 63 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4505 4522 4675 4860 5305 325 Size: 1255 2925 17 153 185 445 1498 Fragments arranged by size: 2925 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 3336 4724 Size: 274 80 33 51 1443 408 951 1388 Cuts at: 4724 6436 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1388 951 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2233 2265 6438 6470 2233 Size: 32 4173 32 2241 Fragments arranged by size: 4173 2241 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6478 XbaI T'CTAG_A Cuts at: 2875 3814 2875 Size: 939 5539 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2532 3406 2532 Size: 874 5604 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6478 XmaI C'CCGG_G Cuts at: 1870 3803 1870 Size: 1933 4545 XmnI GAAnn'nnTTC Cuts at: 2702 3113 3277 3675 4607 2702 Size: 411 164 398 932 4573 Fragments arranged by size: 4573 932 411 398 164 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4286 276 Size: 53 83 186 936 2752 2468 Fragments arranged by size: 2752 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI