(Linear) (Six-base) MAPSORT of: KIEE2205.seq Check: 1208 from: 1 to: 1773 KIEE2205.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AccI GT'mk_AC Cuts at: 0 488 1773 Size: 488 1285 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 1210 1342 1639 1773 Size: 103 1107 132 297 134 Fragments arranged by size: 1107 297 134 132 103 AflIII A'CryG_T Cuts at: 0 1572 1773 Size: 1572 201 AleI CACnn'nnGTG Cuts at: 0 376 1773 Size: 376 1397 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1114 1146 1773 Size: 1114 32 627 Fragments arranged by size: 1114 627 32 AlwNI CAG_nnn'CTG Cuts at: 0 1050 1311 1320 1495 1773 Size: 1050 261 9 175 278 Fragments arranged by size: 1050 278 261 175 9 ApaI G_GGCC'C Cuts at: 0 28 1152 1614 1773 Size: 28 1124 462 159 Fragments arranged by size: 1124 462 159 28 ApoI r'AATT_y Cuts at: 0 796 1188 1773 Size: 796 392 585 Fragments arranged by size: 796 585 392 BanI G'GyrC_C Cuts at: 0 1341 1773 Size: 1341 432 BanII G_rGCy'C Cuts at: 0 28 1152 1614 1773 Size: 28 1124 462 159 Fragments arranged by size: 1124 462 159 28 BbsI GAAGACnn'nnnn_ Cuts at: 0 696 1086 1642 1773 Size: 696 390 556 131 Fragments arranged by size: 696 556 390 131 BbvCI CC'TCA_GC Cuts at: 0 814 1773 Size: 814 959 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 357 391 1773 Size: 357 34 1382 Fragments arranged by size: 1382 357 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1349 1773 Size: 1349 424 BclI T'GATC_A Cuts at: 0 1428 1773 Size: 1428 345 Bme1580I G_kGCm'C Cuts at: 0 28 1152 1614 1773 Size: 28 1124 462 159 Fragments arranged by size: 1124 462 159 28 BmrI ACTGGGnnnn_n' Cuts at: 0 1146 1608 1773 Size: 1146 462 165 Fragments arranged by size: 1146 462 165 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 351 1773 Size: 351 1422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1027 1773 Size: 1027 746 Bpu10I CC'TnA_GC Cuts at: 0 364 814 1773 Size: 364 450 959 Fragments arranged by size: 959 450 364 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1660 1773 Size: 1660 113 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 456 1549 1773 Size: 456 1093 224 Fragments arranged by size: 1093 456 224 BseYI C'CCAG_C Cuts at: 0 175 1773 Size: 175 1598 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1042 1045 1773 Size: 1042 3 728 Fragments arranged by size: 1042 728 3 BsmI GAATG_Cn' Cuts at: 0 848 1773 Size: 848 925 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1300 1773 Size: 1300 473 Bsp1286I G_dGCh'C Cuts at: 0 28 1152 1614 1773 Size: 28 1124 462 159 Fragments arranged by size: 1124 462 159 28 BspHI T'CATG_A Cuts at: 0 1097 1773 Size: 1097 676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1067 1773 Size: 1067 706 BsrBI CCG'CTC Cuts at: 0 718 1773 Size: 718 1055 BsrGI T'GTAC_A Cuts at: 0 380 1773 Size: 380 1393 BstAPI GCAn_nnn'nTGC Cuts at: 0 1495 1773 Size: 1495 278 BstEII G'GTnAC_C Cuts at: 0 1288 1773 Size: 1288 485 BstYI r'GATC_y Cuts at: 0 86 341 915 1773 Size: 86 255 574 858 Fragments arranged by size: 858 574 255 86 BtgI C'CryG_G Cuts at: 0 413 1031 1773 Size: 413 618 742 Fragments arranged by size: 742 618 413 BtsI GCAGTG_nn' Cuts at: 0 581 915 980 1773 Size: 581 334 65 793 Fragments arranged by size: 793 581 334 65 DraIII CAC_nnn'GTG Cuts at: 0 432 1773 Size: 432 1341 DrdI GACnn_nn'nnGTC Cuts at: 0 1431 1773 Size: 1431 342 EaeI y'GGCC_r Cuts at: 0 353 1773 Size: 353 1420 EarI CTCTTCn'nnn_ Cuts at: 0 755 1552 1773 Size: 755 797 221 Fragments arranged by size: 797 755 221 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 1027 1210 1342 1639 1773 Size: 103 924 183 132 297 134 Fragments arranged by size: 924 297 183 134 132 103 EcoNI CCTnn'n_nnAGG Cuts at: 0 1675 1773 Size: 1675 98 EcoO109I rG'GnC_Cy Cuts at: 0 297 1148 1610 1773 Size: 297 851 462 163 Fragments arranged by size: 851 462 297 163 EcoRI G'AATT_C Cuts at: 0 1188 1773 Size: 1188 585 EcoRV GAT'ATC Cuts at: 0 1728 1773 Size: 1728 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 563 595 1629 1661 1773 Size: 563 32 1034 32 112 Fragments arranged by size: 1034 563 112 32 32 HindIII A'AGCT_T Cuts at: 0 645 1773 Size: 645 1128 MfeI C'AATT_G Cuts at: 0 1582 1773 Size: 1582 191 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1136 1490 1773 Size: 1136 354 283 Fragments arranged by size: 1136 354 283 MscI TGG'CCA Cuts at: 0 355 1773 Size: 355 1418 MslI CAynn'nnrTG Cuts at: 0 376 528 824 1773 Size: 376 152 296 949 Fragments arranged by size: 949 376 296 152 MspA1I CmG'CkG Cuts at: 0 60 499 1026 1773 Size: 60 439 527 747 Fragments arranged by size: 747 527 439 60 NcoI C'CATG_G Cuts at: 0 413 1773 Size: 413 1360 NdeI CA'TA_TG Cuts at: 0 1720 1773 Size: 1720 53 NspI r_CATG'y Cuts at: 0 1576 1773 Size: 1576 197 PciI A'CATG_T Cuts at: 0 1572 1773 Size: 1572 201 PflMI CCAn_nnn'nTGG Cuts at: 0 859 1620 1773 Size: 859 761 153 Fragments arranged by size: 859 761 153 PfoI T'CCnGG_A Cuts at: 0 337 1043 1773 Size: 337 706 730 Fragments arranged by size: 730 706 337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1658 1690 1773 Size: 1658 32 83 Fragments arranged by size: 1658 83 32 PshAI GACnn'nnGTC Cuts at: 0 892 1773 Size: 892 881 PspOMI G'GGCC_C Cuts at: 0 24 1148 1610 1773 Size: 24 1124 462 163 Fragments arranged by size: 1124 462 163 24 PstI C_TGCA'G Cuts at: 0 1316 1491 1773 Size: 1316 175 282 Fragments arranged by size: 1316 282 175 PvuII CAG'CTG Cuts at: 0 60 499 1026 1773 Size: 60 439 527 747 Fragments arranged by size: 747 527 439 60 SapI GCTCTTCn'nnn_ Cuts at: 0 755 1773 Size: 755 1018 ScaI AGT'ACT Cuts at: 0 1333 1773 Size: 1333 440 SexAI A'CCwGG_T Cuts at: 0 471 1337 1773 Size: 471 866 436 Fragments arranged by size: 866 471 436 SfcI C'TryA_G Cuts at: 0 485 1312 1420 1487 1773 Size: 485 827 108 67 286 Fragments arranged by size: 827 485 286 108 67 SmlI C'TyrA_G Cuts at: 0 1675 1773 Size: 1675 98 SspI AAT'ATT Cuts at: 0 1384 1773 Size: 1384 389 StyI C'CwwG_G Cuts at: 0 224 350 413 947 1345 1394 1773 Size: 224 126 63 534 398 49 379 Fragments arranged by size: 534 398 379 224 126 63 49 TatI w'GTAC_w Cuts at: 0 380 1331 1773 Size: 380 951 442 Fragments arranged by size: 951 442 380 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 228 260 1773 Size: 228 32 1513 Fragments arranged by size: 1513 228 32 XbaI T'CTAG_A Cuts at: 0 870 1773 Size: 870 903 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 527 1401 1773 Size: 527 874 372 Fragments arranged by size: 874 527 372 XmnI GAAnn'nnTTC Cuts at: 0 697 1108 1272 1670 1773 Size: 697 411 164 398 103 Fragments arranged by size: 697 411 398 164 103 Enzymes that do cut: AccI AcuI AflIII AleI AloI AlwNI ApaI ApoI BanI BanII BbsI BbvCI BcgI BciVI BclI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrGI BstAPI BstEII BstYI BtgI BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI PpiI PshAI PspOMI PstI PvuII SapI ScaI SexAI SfcI SmlI SspI StyI TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BfrBI BglI BglII BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BspEI BsrDI BsrFI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI EciI EcoICRI FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I XhoI XmaI ZraI