Restriction map for
pFN21AE3240
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MspA1I MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 904 CGCG CG^CG
AocI 1307 CCTCAGG CC^T[ACGT]AGG
BalI 1346 TGGCCA TGG^CCA
BanI 1302 GGTGCC G^G[CT][GA]CC
BbsI 192 GAAGAC GAAGAC(2/6)
BbvCI 46 CCTCAGC CCTCAGC(-5/-2)
BciVI 168 GGATAC GTATCC(6/5)
BclI 1271 TGATCA T^GATCA
BcnI 32 CCCGG CC^[GC]GG
BfaI 1552 CTAG C^TAG
BglI 1339 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 192 GAAGAC GAAGAC(2/6)
BseRI 358 GAGGAG GAGGAG(10/8)
BsiEI 900 CGACCG CG[GA][CT]^CG
BsmFI 1358 GGGAC GGGAC(10/14)
BsmI 840 GAATGC GAATGC(1/-1)
BspHI 884 TCATGA T^CATGA
BspMI 979 GCAGGT ACCTGC(4/8)
BsrFI 287 ACCGGC [GA]^CCGG[CT]
BstUI 904 CGCG CG^CG
Bsu36I 1307 CCTCAGG CC^T[ACGT]AGG
CfoI 1548 GCGC GCG^C
Cfr10I 287 ACCGGC [GA]^CCGG[CT]
DsaI 1340 CCATGG C^C[GA][CT]GG
EaeI 1346 TGGCCA [CT]^GGCC[GA]
Eco81I 1307 CCTCAGG CC^T[ACGT]AGG
FbaI 1271 TGATCA T^GATCA
HaeII 1547 AGCGCC [GA]GCGC^[CT]
HhaI 1548 GCGC GCG^C
HinP1I 1548 GCGC G^CGC
HincII 943 GTTGAC GT[CT]^[GA]AC
HindII 943 GTTGAC GT[CT]^[GA]AC
MaeI 1552 CTAG C^TAG
MluNI 1346 TGGCCA TGG^CCA
MscI 1346 TGGCCA TGG^CCA
MvnI 904 CGCG CG^CG
NciI 32 CCCGG CC^[GC]GG
NcoI 1340 CCATGG C^CATGG
RcaI 884 TCATGA T^CATGA
SexAI 1298 ACCTGGT A^CC[AT]GGT
SfiI 1338 GGCCATGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XcmI 17 CCAAGGAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AlwNI 1037 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1250 CAGAATCTG
AspHI 1225 GAGCTC G[AT]GC[AT]^C
1324 GAGCTC
AvaI 1354 CTCGGG C^[CT]CG[GA]G
1391 CTCGGG
BpmI 566 CTGGAG CTGGAG(16/14)
1019 CTGGAG
BsaI 1295 GAGACC GGTCTC(1/5)
1433 GAGACC
BsgI 1662 CTGCAC GTGCAG(16/14)
770 GTGCAG
BsiHKAI 1225 GAGCTC G[AT]GC[AT]^C
1324 GAGCTC
BsoBI 1354 CTCGGG C^[CT]CG[GA]G
1391 CTCGGG
BstF5I 1575 CATCC GGATG(2/0)
912 GGATG
DraIII 1170 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
1172 CACAGTGTG
EarI 634 GAAGAG CTCTTC(1/4)
1411 GAAGAG
EcoNI 1230 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1329 CCTGAAGGAGG
EcoT14I 17 CCAAGG C^C[AT][AT]GG
1340 CCATGG
FokI 1575 CATCC GGATG(9/13)
912 GGATG
HgaI 1421 GACGC GACGC(5/10)
1570 GCGTC
Ksp632I 634 GAAGAG CTCTTC(1/4)
1411 GAAGAG
MaeII 1290 ACGT A^CGT
1646 ACGT
MwoI 1339 GCCATGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1502 GCCGGAGCAGC
PflMI 205 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
421 CCAGGAAGTGG
PstI 344 CTGCAG CTGCA^G
974 CTGCAG
SacI 1225 GAGCTC GAGCT^C
1324 GAGCTC
SfcI 344 CTGCAG C^T[GA][CT]AG
974 CTGCAG
StyI 17 CCAAGG C^C[AT][AT]GG
1340 CCATGG
TaqI 390 TCGA T^CGA
1003 TCGA
TfiI 470 GATTC G^A[AT]TC
1252 GAATC
TspRI 1017 CACTG CA[GC]TG[ACGT][ACGT]^
1174 CAGTG
TthHB8I 390 TCGA T^CGA
1003 TCGA
Van91I 205 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
421 CCAGGAAGTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
Tsp509I XhoII