(Circular) (Six-base) MAPSORT of: pFN21AE3240.seq Check: 7120 from: 1 to: 6391 pFN21AE3240 6391 bp With 182 enzymes: SgfI * March 28, 2012 12:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4201 278 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3734 1937 Size: 1797 4594 Acc65I G'GTAC_C Cuts at: 3712 3712 Size: 6391 AclI AA'CG_TT Cuts at: 1563 4518 4891 1563 Size: 2955 373 3063 Fragments arranged by size: 3063 2955 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2789 3356 4454 5838 6106 1632 Size: 1157 567 1098 1384 268 1917 Fragments arranged by size: 1917 1384 1157 1098 567 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6194 AflIII A'CryG_T Cuts at: 1203 5290 6267 1203 Size: 4087 977 1327 Fragments arranged by size: 4087 1327 977 AgeI A'CCGG_T Cuts at: 5222 5222 Size: 6391 AhdI GACnn_n'nnGTC Cuts at: 5120 5120 Size: 6391 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2865 1184 Size: 1681 4710 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2909 2941 2909 Size: 32 6359 AlwNI CAG_nnn'CTG Cuts at: 3047 3260 4134 5706 3047 Size: 213 874 1572 3732 Fragments arranged by size: 3732 1572 874 213 ApaLI G'TGCA_C Cuts at: 4448 5604 4448 Size: 1156 5235 ApoI r'AATT_y Cuts at: 1493 3700 3926 4078 6187 1493 Size: 2207 226 152 2109 1697 Fragments arranged by size: 2207 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4945 160 Size: 872 3913 1606 Fragments arranged by size: 3913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 AvaI C'yCGr_G Cuts at: 1870 1943 3359 3396 3716 1870 Size: 73 1416 37 320 4545 Fragments arranged by size: 4545 1416 320 73 37 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2166 2199 2166 Size: 33 6358 BamHI G'GATC_C Cuts at: 3721 4088 3721 Size: 367 6024 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3307 3712 5167 618 Size: 325 217 627 1520 405 1455 1842 Fragments arranged by size: 1842 1520 1455 627 405 325 217 BanII G_rGCy'C Cuts at: 1391 2886 3234 3333 3710 1391 Size: 1495 348 99 377 4072 Fragments arranged by size: 4072 1495 377 348 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2204 928 Size: 971 305 5115 Fragments arranged by size: 5115 971 305 BbvCI CC'TCA_GC Cuts at: 2052 2052 Size: 6391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4582 4616 1903 Size: 34 2645 34 3678 Fragments arranged by size: 3678 2645 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2167 4283 5493 2167 Size: 2116 1210 3065 Fragments arranged by size: 3065 2116 1210 BclI T'GATC_A Cuts at: 1548 3276 1548 Size: 1728 4663 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3350 5002 136 Size: 107 122 71 631 605 1678 1652 1525 Fragments arranged by size: 1678 1652 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6386 6386 Size: 6391 BlpI GC'TnA_GC Cuts at: 3808 3808 Size: 6391 Bme1580I G_kGCm'C Cuts at: 1790 3312 4452 5608 1790 Size: 1522 1140 1156 2573 Fragments arranged by size: 2573 1522 1156 1140 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2156 4122 5080 Size: 416 53 83 399 24 739 1966 958 Cuts at: 5080 442 Size: 1753 Fragments arranged by size: 1966 1753 958 739 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2592 3045 5051 1239 Size: 1353 453 2006 2579 Fragments arranged by size: 2579 2006 1353 453 Bpu10I CC'TnA_GC Cuts at: 2052 2052 Size: 6391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4516 5381 5679 4516 Size: 865 298 5228 Fragments arranged by size: 5228 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3294 3432 5054 882 Size: 617 1795 138 1622 2219 Fragments arranged by size: 2219 1795 1622 617 138 BsaAI yAC'GTr Cuts at: 493 1204 4182 493 Size: 711 2978 2702 Fragments arranged by size: 2978 2702 711 BsaBI GATnn'nnATC Cuts at: 6385 6385 Size: 6391 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4198 4580 5215 275 Size: 53 83 186 936 2665 382 635 1451 Fragments arranged by size: 2665 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4824 5222 5496 5643 6054 4824 Size: 398 274 147 411 5161 Fragments arranged by size: 5161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2191 2221 3291 3321 1178 Size: 30 165 30 788 30 1070 30 4248 Fragments arranged by size: 4248 1070 788 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2378 1186 Size: 1192 5199 BseYI C'CCAG_C Cuts at: 5594 6090 5594 Size: 496 5895 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2796 3652 1163 Size: 573 1060 856 3902 Fragments arranged by size: 3902 1060 856 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 3841 4602 5630 1540 Size: 275 186 907 933 761 1028 2301 Fragments arranged by size: 2301 1028 933 907 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3234 3333 4452 4537 5608 3234 Size: 99 1119 85 1071 4017 Fragments arranged by size: 4017 1119 1071 99 85 BsmI GAATG_Cn' Cuts at: 2851 3902 3995 2851 Size: 1051 93 5247 Fragments arranged by size: 5247 1051 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2886 3234 3312 3333 3710 4452 4537 Size: 399 1096 348 78 21 377 742 85 Cuts at: 4537 5608 1391 Size: 1071 2174 Fragments arranged by size: 2174 1096 1071 742 399 377 348 85 78 21 BspEI T'CCGG_A Cuts at: 6054 6054 Size: 6391 BspHI T'CATG_A Cuts at: 2889 4280 6010 2889 Size: 1391 1730 3270 Fragments arranged by size: 3270 1730 1391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 3746 844 Size: 2131 771 3489 Fragments arranged by size: 3489 2131 771 BsrBI CCG'CTC Cuts at: 1394 1823 4278 1394 Size: 429 2455 3507 Fragments arranged by size: 3507 2455 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4886 5060 65 Size: 1183 3638 174 1396 Fragments arranged by size: 3638 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2292 5035 5222 1700 Size: 110 482 2743 187 2869 Fragments arranged by size: 2869 2743 482 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6391 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6391 BssSI C'ACGA_G Cuts at: 4451 5463 4451 Size: 1012 5379 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6391 BstBI TT'CG_AA Cuts at: 5195 5195 Size: 6391 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6391 BstYI r'GATC_y Cuts at: 1972 2101 2234 2335 2514 3044 3721 4088 4473 Size: 129 133 101 179 530 677 367 385 Cuts at: 4473 4490 5942 6028 6040 6386 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 677 530 385 367 346 179 133 129 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3313 3313 Size: 6391 BtgI C'CryG_G Cuts at: 513 1065 3345 513 Size: 552 2280 3559 Fragments arranged by size: 3559 2280 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2925 3529 487 Size: 683 56 1699 604 3349 Fragments arranged by size: 3349 1699 683 604 56 BtsI GCAGTG_nn' Cuts at: 3907 4701 4721 3907 Size: 794 20 5577 Fragments arranged by size: 5577 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6391 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6356 DraI TTT'AAA Cuts at: 3695 3695 Size: 6391 DraIII CAC_nnn'GTG Cuts at: 3180 3182 3180 Size: 2 6389 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5398 809 Size: 507 4082 1802 Fragments arranged by size: 4082 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3351 3838 4727 Size: 54 1425 50 275 18 1521 487 889 Cuts at: 4727 6356 8 Size: 1629 43 Fragments arranged by size: 1629 1521 1425 889 487 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3838 1537 Size: 275 2026 4090 Fragments arranged by size: 4090 2026 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2634 3411 4321 1353 Size: 1281 777 910 3423 Fragments arranged by size: 3423 1281 910 777 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4978 5352 5498 387 Size: 1064 138 3389 374 146 1280 Fragments arranged by size: 3389 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3232 3331 3232 Size: 99 6292 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2592 2789 3045 3356 4454 5051 5838 Size: 393 960 197 256 311 1098 597 787 Cuts at: 5838 6106 1239 Size: 268 1524 Fragments arranged by size: 1524 1098 960 787 597 393 311 268 256 197 EcoNI CCTnn'n_nnAGG Cuts at: 3239 3338 3239 Size: 99 6292 EcoRI G'AATT_C Cuts at: 3700 4078 3700 Size: 378 6013 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2218 2218 Size: 6391 FspI TGC'GCA Cuts at: 4897 4897 Size: 6391 HaeII r_GCGC'y Cuts at: 1678 3556 5538 1678 Size: 1878 1982 2531 Fragments arranged by size: 2531 1982 1878 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2191 2223 2388 2420 3029 Size: 32 135 32 619 32 165 32 609 Cuts at: 3029 3061 3564 3596 5032 5064 5106 5138 1373 Size: 32 503 32 1436 32 42 32 2626 Fragments arranged by size: 2626 1436 619 609 503 165 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2950 3735 3981 669 Size: 988 281 1012 785 246 3079 Fragments arranged by size: 3079 1012 988 785 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6391 HpaI GTT'AAC Cuts at: 3981 3981 Size: 6391 KpnI G_GTAC'C Cuts at: 3716 3716 Size: 6391 MfeI C'AATT_G Cuts at: 3990 3990 Size: 6391 MluI A'CGCG_T Cuts at: 6267 6267 Size: 6391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5505 5689 1305 Size: 4200 184 2007 Fragments arranged by size: 4200 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3353 10 Size: 54 1425 343 1521 3048 Fragments arranged by size: 3048 1521 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 2939 3584 4349 4708 4867 518 Size: 648 1323 450 645 765 359 159 2042 Fragments arranged by size: 2042 1323 765 648 645 450 359 159 MspA1I CmG'CkG Cuts at: 1606 3807 4484 5632 5877 1606 Size: 2201 677 1148 245 2120 Fragments arranged by size: 2201 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3345 513 Size: 552 2280 3559 Fragments arranged by size: 3559 2280 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6391 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6391 NotI GC'GGCC_GC Cuts at: 3838 3838 Size: 6391 NspI r_CATG'y Cuts at: 1132 1229 3749 5294 1132 Size: 97 2520 1545 2229 Fragments arranged by size: 2520 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6391 PciI A'CATG_T Cuts at: 5290 5290 Size: 6391 PflMI CCAn_nnn'nTGG Cuts at: 1108 2216 2432 1108 Size: 1108 216 5067 Fragments arranged by size: 5067 1108 216 PfoI T'CCnGG_A Cuts at: 2425 2871 2425 Size: 446 5945 PmeI GTTT'AAAC Cuts at: 3695 3695 Size: 6391 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4460 4492 5973 6005 4460 Size: 32 1481 32 4846 Fragments arranged by size: 4846 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6391 PsiI TTA'TAA Cuts at: 3961 3961 Size: 6391 PstI C_TGCA'G Cuts at: 830 2353 2983 3743 830 Size: 1523 630 760 3478 Fragments arranged by size: 3478 1523 760 630 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6391 SacI G_AGCT'C Cuts at: 3234 3333 3234 Size: 99 6292 SalI G'TCGA_C Cuts at: 1936 3733 1936 Size: 1797 4594 SbfI CC_TGCA'GG Cuts at: 3743 3743 Size: 6391 ScaI AGT'ACT Cuts at: 4639 6351 4639 Size: 1712 4679 SexAI A'CCwGG_T Cuts at: 3303 3303 Size: 6391 SfcI C'TryA_G Cuts at: 826 1045 2349 2979 3739 4874 5555 5746 826 Size: 219 1304 630 760 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 760 681 630 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3350 3350 Size: 6391 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6391 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6391 SmaI CCC'GGG Cuts at: 1872 3718 1872 Size: 1846 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4495 5396 5658 820 Size: 197 926 2552 901 262 1553 Fragments arranged by size: 2552 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4182 493 Size: 3689 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6391 SphI G_CATG'C Cuts at: 1132 3749 1132 Size: 2617 3774 SspI AAT'ATT Cuts at: 5 52 4315 5 Size: 47 4263 2081 Fragments arranged by size: 4263 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6391 StyI C'CwwG_G Cuts at: 513 1065 2022 3345 3830 513 Size: 552 957 1323 485 3074 Fragments arranged by size: 3074 1323 957 552 485 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 4418 4435 4588 4773 5218 325 Size: 1255 558 2280 17 153 185 445 1498 Fragments arranged by size: 2280 1498 1255 558 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4637 6349 96 Size: 274 80 33 51 1443 2660 1712 138 Fragments arranged by size: 2660 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3291 3323 6351 6383 3291 Size: 32 3028 32 3299 Fragments arranged by size: 3299 3028 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6391 XbaI T'CTAG_A Cuts at: 3727 3727 Size: 6391 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 6391 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6391 XmaI C'CCGG_G Cuts at: 1870 3716 1870 Size: 1846 4545 XmnI GAAnn'nnTTC Cuts at: 4520 4520 Size: 6391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4199 276 Size: 53 83 186 936 2665 2468 Fragments arranged by size: 2665 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfoI SrfI SwaI