Restriction map for
pFN21AE3079
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbsI BcgI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp45I
TspRI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 597 GTAC GT^AC
AflIII 691 ACATGT A^C[GA][CT]GT
AspHI 241 GAGCTC G[AT]GC[AT]^C
AvaII 86 GGACC G^G[AT]CC
BamHI 614 GGATCC G^GATCC
BanI 93 GGCACC G^G[CT][GA]CC
BbvCI 147 GCTGAGG CCTCAGC(-5/-2)
BciVI 33 GTATCC GTATCC(6/5)
BglI 154 GCCATGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 262 GAGACC GGTCTC(1/5)
BseRI 460 GAGGAG GAGGAG(10/8)
BsiHKAI 241 GAGCTC G[AT]GC[AT]^C
BsmAI 262 GAGAC GTCTC(1/5)
BspDI 628 ATCGAT AT^CGAT
BspLU11I 691 ACATGT A^CATGT
BsrFI 76 GCCGGT [GA]^CCGG[CT]
BssSI 457 CACGAG CACGAG(-5/-1)
BstNI 90 CCAGG CC^[AT]GG
BstYI 614 GGATCC [GA]^GATC[CT]
Cfr10I 76 GCCGGT [GA]^CCGG[CT]
ClaI 628 ATCGAT AT^CGAT
DsaI 155 CCATGG C^C[GA][CT]GG
EaeI 444 TGGCCG [CT]^GGCC[GA]
EarI 348 GAAGAG CTCTTC(1/4)
EcoRI 379 GAATTC G^AATTC
EcoRII 90 CCAGG ^CC[AT]GG
EcoT14I 155 CCATGG C^C[AT][AT]GG
EcoT22I 632 ATGCAT ATGCA^T
HindIII 361 AAGCTT A^AGCTT
Ksp632I 348 GAAGAG CTCTTC(1/4)
MflI 614 GGATCC [GA]^GATC[CT]
MspA1I 17 CAGCGG C[AC]G^C[GT]G
MvaI 90 CCAGG CC^[AT]GG
NcoI 155 CCATGG C^CATGG
NsiI 632 ATGCAT ATGCA^T
PleI 394 GACTC GAGTC(4/5)
PpuMI 85 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 597 GTAC GT^AC
SacI 241 GAGCTC GAGCT^C
ScaI 596 AGTACT AGT^ACT
SfcI 600 CTACAG C^T[GA][CT]AG
SmaI 70 CCCGGG CCC^GGG
StuI 542 AGGCCT AGG^CCT
StyI 155 CCATGG C^C[AT][AT]GG
XcmI 126 CCATTTAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 614 GGATCC [GA]^GATC[CT]
XmaCI 70 CCCGGG C^CCGGG
XmaI 70 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 255 GAATTT [GA]^AATT[CT]
379 GAATTC
ApoI 255 GAATTT [GA]^AATT[CT]
379 GAATTC
AvaI 28 CCCGAG C^[CT]CG[GA]G
70 CCCGGG
BanII 241 GAGCTC G[GA]GC[CT]^C
481 GAGCCC
BbvI 673 GCAGC GCAGC(8/12)
57 GCTGC
BfaI 178 CTAG C^TAG
670 CTAG
BmyI 241 GAGCTC G[AGT]GC[ACT]^C
481 GAGCCC
BsmFI 85 GGGAC GGGAC(10/14)
140 GGGAC
BsoBI 28 CCCGAG C^[CT]CG[GA]G
70 CCCGGG
Bsp1286I 241 GAGCTC G[AGT]GC[ACT]^C
481 GAGCCC
BsrI 136 ACTGG ACTGG(1/-1)
216 ACTGG
DdeI 148 CTGAG C^T[ACGT]AG
171 CTCAG
DraII 63 GGGGCCT [GA]G^G[ACGT]CC[CT]
85 GGGACCC
EcoO109I 63 GGGGCCT [GA]G^G[ACGT]CC[CT]
85 GGGACCC
MaeI 178 CTAG C^TAG
670 CTAG
NspI 143 ACATGC [GA]CATG^[CT]
691 ACATGT
TseI 57 GCTGC G^C[AT]GC
673 GCAGC
Tsp509I 256 AATT ^AATT
380 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwI BcnI BsaJI
BsiYI BslI BssKI BstF5I Cac8I
Cfr13I DpnII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MnlI MspI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TthHB8I