(Circular) (Six-base) MAPSORT of: pFN21AE3079.seq Check: 8619 from: 1 to: 5422 pFN21AE3079 5422 bp With 182 enzymes: SgfI * March 28, 2012 12:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2743 2743 Size: 5422 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2216 3485 4869 5137 1632 Size: 584 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 584 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 2696 4321 5298 1203 Size: 1493 1625 977 1327 Fragments arranged by size: 1625 1493 1327 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5422 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5422 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2505 2537 2505 Size: 32 5390 AlwNI CAG_nnn'CTG Cuts at: 3165 4737 3165 Size: 1572 3850 ApaLI G'TGCA_C Cuts at: 3479 4635 3479 Size: 1156 4266 ApoI r'AATT_y Cuts at: 1493 2260 2384 2731 2957 3109 5218 1493 Size: 767 124 347 226 152 2109 1697 Fragments arranged by size: 2109 1697 767 347 226 152 124 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2075 2747 1870 Size: 73 90 42 672 4545 Fragments arranged by size: 4545 672 90 73 42 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2021 2054 2021 Size: 33 5389 BamHI G'GATC_C Cuts at: 2619 2752 3119 2619 Size: 133 367 4922 Fragments arranged by size: 4922 367 133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 2743 4198 618 Size: 325 217 627 311 645 1455 1842 Fragments arranged by size: 1842 1455 645 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2250 2490 2741 1391 Size: 859 240 251 4072 Fragments arranged by size: 4072 859 251 240 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4451 BbvCI CC'TCA_GC Cuts at: 2153 2153 Size: 5422 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3613 3647 1903 Size: 34 1676 34 3678 Fragments arranged by size: 3678 1676 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2049 3314 4524 2049 Size: 1265 1210 2947 Fragments arranged by size: 2947 1265 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5422 BfrBI ATG'CAT Cuts at: 2639 2639 Size: 5422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2165 4033 136 Size: 107 122 71 631 605 493 1868 1525 Fragments arranged by size: 1868 1525 631 605 493 122 107 71 BglII A'GATC_T Cuts at: 5417 5417 Size: 5422 BlpI GC'TnA_GC Cuts at: 2839 2839 Size: 5422 Bme1580I G_kGCm'C Cuts at: 1790 3483 4639 1790 Size: 1693 1156 2573 Fragments arranged by size: 2573 1693 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5422 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2151 3153 4111 Size: 416 53 83 399 24 734 1002 958 Cuts at: 4111 442 Size: 1753 Fragments arranged by size: 1753 1002 958 734 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4082 1239 Size: 2843 2579 Bpu10I CC'TnA_GC Cuts at: 2153 2153 Size: 5422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3547 4412 4710 3547 Size: 865 298 4259 Fragments arranged by size: 4259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2261 4085 882 Size: 617 762 1824 2219 Fragments arranged by size: 2219 1824 762 617 BsaAI yAC'GTr Cuts at: 493 1204 3213 493 Size: 711 2009 2702 Fragments arranged by size: 2702 2009 711 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3229 3611 4246 275 Size: 53 83 186 936 1696 382 635 1451 Fragments arranged by size: 1696 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3855 4253 4527 4674 5085 3855 Size: 398 274 147 411 4192 Fragments arranged by size: 4192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5197 Fragments arranged by size: 5197 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2480 1186 Size: 1294 4128 BseYI C'CCAG_C Cuts at: 2012 2065 2213 4625 5121 2012 Size: 53 148 2412 496 2313 Fragments arranged by size: 2412 2313 496 148 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4849 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2872 3633 4661 1540 Size: 275 186 871 761 1028 2301 Fragments arranged by size: 2301 1028 871 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 3483 3568 4639 2250 Size: 1233 85 1071 3033 Fragments arranged by size: 3033 1233 1071 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2490 2741 3483 3568 4639 1391 Size: 399 460 240 251 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 460 399 251 240 85 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 844 Size: 1933 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3309 1394 Size: 429 1486 3507 Fragments arranged by size: 3507 1486 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3917 4091 65 Size: 1183 2669 174 1396 Fragments arranged by size: 2669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2081 4066 4253 1700 Size: 110 271 1985 187 2869 Fragments arranged by size: 2869 1985 271 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 2462 3482 4494 2462 Size: 1020 1012 3390 Fragments arranged by size: 3390 1020 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5422 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2619 2752 3119 3504 3521 4973 5059 5071 Size: 647 133 367 385 17 1452 86 12 Cuts at: 5071 5417 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 647 385 367 346 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2160 513 Size: 552 939 156 3775 Fragments arranged by size: 3775 939 552 156 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4683 Fragments arranged by size: 4683 683 56 BtsI GCAGTG_nn' Cuts at: 2938 3732 3752 2938 Size: 794 20 4608 Fragments arranged by size: 4608 794 20 ClaI AT'CG_AT Cuts at: 1554 2634 1554 Size: 1080 4342 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2449 2869 3758 Size: 54 1425 50 275 18 619 420 889 Cuts at: 3758 5387 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 619 420 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2869 1537 Size: 275 1057 4090 Fragments arranged by size: 4090 1057 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2348 3352 1353 Size: 995 1004 3423 Fragments arranged by size: 3423 1004 995 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2549 2617 4009 4383 4529 387 Size: 1064 138 960 68 1392 374 146 1280 Fragments arranged by size: 1392 1280 1064 960 374 146 138 68 EcoICRI GAG'CTC Cuts at: 2248 2248 Size: 5422 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 3485 4082 4869 5137 1239 Size: 393 584 1269 597 787 268 1524 Fragments arranged by size: 1524 1269 787 597 584 393 268 EcoO109I rG'GnC_Cy Cuts at: 2069 2091 2069 Size: 22 5400 EcoRI G'AATT_C Cuts at: 2384 2731 3109 2384 Size: 347 378 4697 Fragments arranged by size: 4697 378 347 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2384 2384 Size: 5422 FspI TGC'GCA Cuts at: 3928 3928 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 4569 1678 Size: 2891 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373 Size: 32 135 32 2491 32 42 32 2626 Fragments arranged by size: 2626 2491 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2766 3012 669 Size: 988 281 828 246 3079 Fragments arranged by size: 3079 988 828 281 246 HindIII A'AGCT_T Cuts at: 748 2366 748 Size: 1618 3804 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KpnI G_GTAC'C Cuts at: 2747 2747 Size: 5422 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4536 4720 1305 Size: 3231 184 2007 Fragments arranged by size: 3231 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3600 Fragments arranged by size: 3600 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3380 3739 3898 518 Size: 648 2214 359 159 2042 Fragments arranged by size: 2214 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2024 2838 3515 4663 4908 1606 Size: 418 814 677 1148 245 2120 Fragments arranged by size: 2120 1148 814 677 418 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2160 513 Size: 552 939 156 3775 Fragments arranged by size: 3775 939 552 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2869 2869 Size: 5422 NsiI A_TGCA'T Cuts at: 2641 2641 Size: 5422 NspI r_CATG'y Cuts at: 1132 1229 2152 2700 2780 4325 1132 Size: 97 923 548 80 1545 2229 Fragments arranged by size: 2229 1545 923 548 97 80 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5422 PciI A'CATG_T Cuts at: 2696 4321 2696 Size: 1625 3797 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5422 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2437 2469 3491 3523 5004 5036 2437 Size: 32 1022 32 1481 32 2823 Fragments arranged by size: 2823 1481 1022 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2091 2091 Size: 5422 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PstI C_TGCA'G Cuts at: 830 2774 830 Size: 1944 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 SacI G_AGCT'C Cuts at: 2250 2250 Size: 5422 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SanDI GG'GwC_CC Cuts at: 2091 2091 Size: 5422 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 2603 3670 5382 2603 Size: 1067 1712 2643 Fragments arranged by size: 2643 1712 1067 SfcI C'TryA_G Cuts at: 826 1045 2605 2770 3905 4586 4777 826 Size: 219 1560 165 1135 681 191 1471 Fragments arranged by size: 1560 1471 1135 681 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2077 2749 1872 Size: 205 672 4545 Fragments arranged by size: 4545 672 205 SmlI C'TyrA_G Cuts at: 820 1017 1943 3526 4427 4689 820 Size: 197 926 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2780 1132 Size: 1648 3774 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 2549 1517 Size: 1032 4390 StyI C'CwwG_G Cuts at: 513 1065 2004 2160 2861 513 Size: 552 939 156 701 3074 Fragments arranged by size: 3074 939 701 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2116 3449 3466 3619 3804 4249 325 Size: 1255 536 1333 17 153 185 445 1498 Fragments arranged by size: 1498 1333 1255 536 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2601 3668 5380 Size: 274 80 33 51 1443 624 1067 1712 Cuts at: 5380 96 Size: 138 Fragments arranged by size: 1712 1443 1067 624 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5382 5414 5382 Size: 32 5390 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5422 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2138 2138 Size: 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2075 2747 1870 Size: 205 672 4545 Fragments arranged by size: 4545 672 205 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI