Restriction map for
pFN21AE3079
(Linear) (Six-base) MAPSORT of: KIEE3079.seq Check: 8007 from: 1 to: 717
KIEE3079.orf
With 182 enzymes: SgfI *
March 22, 2012 16:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 717
Size: 211 506
AflIII A'CryG_T
Cuts at: 0 691 717
Size: 691 26
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 500 532 717
Size: 500 32 185
Fragments arranged by size:
500 185 32
ApoI r'AATT_y
Cuts at: 0 255 379 717
Size: 255 124 338
Fragments arranged by size:
338 255 124
AvaI C'yCGr_G
Cuts at: 0 28 70 717
Size: 28 42 647
Fragments arranged by size:
647 42 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 16 49 717
Size: 16 33 668
Fragments arranged by size:
668 33 16
BamHI G'GATC_C
Cuts at: 0 614 717
Size: 614 103
BanI G'GyrC_C
Cuts at: 0 93 717
Size: 93 624
BanII G_rGCy'C
Cuts at: 0 245 485 717
Size: 245 240 232
Fragments arranged by size:
245 240 232
BbvCI CC'TCA_GC
Cuts at: 0 148 717
Size: 148 569
BciVI GTATCCnnnnn_n'
Cuts at: 0 44 717
Size: 44 673
BfrBI ATG'CAT
Cuts at: 0 634 717
Size: 634 83
BglI GCCn_nnn'nGGC
Cuts at: 0 160 717
Size: 160 557
BmrI ACTGGGnnnn_n'
Cuts at: 0 146 717
Size: 146 571
Bpu10I CC'TnA_GC
Cuts at: 0 148 717
Size: 148 569
BsaI GGTCTCn'nnnn_
Cuts at: 0 256 717
Size: 256 461
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 717
Size: 475 242
BseYI C'CCAG_C
Cuts at: 0 7 60 208 717
Size: 7 53 148 509
Fragments arranged by size:
509 148 53 7
BsiHKAI G_wGCw'C
Cuts at: 0 245 717
Size: 245 472
Bsp1286I G_dGCh'C
Cuts at: 0 245 485 717
Size: 245 240 232
Fragments arranged by size:
245 240 232
BsrFI r'CCGG_y
Cuts at: 0 76 717
Size: 76 641
BssSI C'ACGA_G
Cuts at: 0 457 717
Size: 457 260
BstYI r'GATC_y
Cuts at: 0 614 717
Size: 614 103
BtgI C'CryG_G
Cuts at: 0 155 717
Size: 155 562
ClaI AT'CG_AT
Cuts at: 0 629 717
Size: 629 88
EaeI y'GGCC_r
Cuts at: 0 444 717
Size: 444 273
EarI CTCTTCn'nnn_
Cuts at: 0 343 717
Size: 343 374
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 544 612 717
Size: 544 68 105
Fragments arranged by size:
544 105 68
EcoICRI GAG'CTC
Cuts at: 0 243 717
Size: 243 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 717
Size: 211 506
EcoO109I rG'GnC_Cy
Cuts at: 0 64 86 717
Size: 64 22 631
Fragments arranged by size:
631 64 22
EcoRI G'AATT_C
Cuts at: 0 379 717
Size: 379 338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 379 717
Size: 379 338
HindIII A'AGCT_T
Cuts at: 0 361 717
Size: 361 356
MspA1I CmG'CkG
Cuts at: 0 19 717
Size: 19 698
NcoI C'CATG_G
Cuts at: 0 155 717
Size: 155 562
NsiI A_TGCA'T
Cuts at: 0 636 717
Size: 636 81
NspI r_CATG'y
Cuts at: 0 147 695 717
Size: 147 548 22
Fragments arranged by size:
548 147 22
PciI A'CATG_T
Cuts at: 0 691 717
Size: 691 26
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 432 464 717
Size: 432 32 253
Fragments arranged by size:
432 253 32
PpuMI rG'GwC_Cy
Cuts at: 0 86 717
Size: 86 631
SacI G_AGCT'C
Cuts at: 0 245 717
Size: 245 472
SanDI GG'GwC_CC
Cuts at: 0 86 717
Size: 86 631
ScaI AGT'ACT
Cuts at: 0 598 717
Size: 598 119
SfcI C'TryA_G
Cuts at: 0 600 717
Size: 600 117
SmaI CCC'GGG
Cuts at: 0 72 717
Size: 72 645
StuI AGG'CCT
Cuts at: 0 544 717
Size: 544 173
StyI C'CwwG_G
Cuts at: 0 155 717
Size: 155 562
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 111 717
Size: 111 606
TatI w'GTAC_w
Cuts at: 0 596 717
Size: 596 121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 133 717
Size: 133 584
XmaI C'CCGG_G
Cuts at: 0 70 717
Size: 70 647
Enzymes that do cut:
AcuI AflIII AloI ApoI AvaI BaeI BamHI BanI
BanII BbvCI BciVI BfrBI BglI BmrI Bpu10I BsaI
BseRI BseYI BsiHKAI Bsp1286I BsrFI BssSI BstYI BtgI
ClaI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI HindIII MspA1I NcoI NsiI NspI PciI PpiI
PpuMI SacI SanDI ScaI SfcI SmaI StuI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BbeI BbsI BcgI BclI BglII BlpI
Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI
DraI DraIII DrdI EagI EcoNI EcoRV FseI FspI
FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmnI ZraI