################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MflI MluI MluNI MroI MscI MseI MslI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SacI SacII SalI SapI Sau3AI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AocI 415 CCTGAGG CC^T[ACGT]AGG BanI 328 GGCACC G^G[CT][GA]CC BbsI 429 GAAGAC GAAGAC(2/6) BpuAI 429 GAAGAC GAAGAC(2/6) BsaAI 366 TACGTA [CT]AC^GT[GA] BseRI 402 CTCCTC GAGGAG(10/8) BsgI 21 CTGCAC GTGCAG(16/14) BsmAI 223 GTCTC GTCTC(1/5) BsmBI 222 CGTCTC CGTCTC(1/5) Bsp1407I 344 TGTACA T^GTACA BspMI 422 ACCTGC ACCTGC(4/8) BsrGI 344 TGTACA T^GTACA BstF5I 360 GGATG GGATG(2/0) Bsu36I 415 CCTGAGG CC^T[ACGT]AGG CpoI 202 CGGACCG CG^G[AT]CCG Eco81I 415 CCTGAGG CC^T[ACGT]AGG FauI 180 CCCGC CCCGC(4/6) FokI 360 GGATG GGATG(9/13) HapII 358 CCGG C^CGG HgaI 12 GCGTC GACGC(5/10) HpaII 358 CCGG C^CGG HphI 128 TCACC GGTGA(8/7) MaeII 367 ACGT A^CGT MfeI 251 CAATTG C^AATTG MspI 358 CCGG C^CGG MunI 251 CAATTG C^AATTG NspI 269 ACATGC [GA]CATG^[CT] PleI 432 GACTC GAGTC(4/5) PpuMI 419 AGGACCT [GA]G^G[AT]CC[CT] PvuII 49 CAGCTG CAG^CTG RsrII 202 CGGACCG CG^G[AT]CCG SbfI 302 CCTGCAGG CCTGCA^GG SfaNI 188 GCATC GCATC(5/9) SnaBI 366 TACGTA TAC^GTA Sse8387I 302 CCTGCAGG CCTGCA^GG SspBI 344 TGTACA T^GTACA TfiI 143 GAATC G^A[AT]TC Tsp509I 252 AATT ^AATT TspRI 264 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 181 CCGC CCGC(-3/-1) 206 CCGC AluI 50 AGCT AG^CT 392 AGCT BsaJI 65 CCCAGG C^C[ACGT][ACGT]GG 131 CCCAGG BsiYI 131 CCCAGGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 404 CCTCCTTTGGG BslI 131 CCCAGGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 404 CCTCCTTTGGG BspHI 314 TCATGA T^CATGA 377 TCATGA BsrI 335 ACTGG ACTGG(1/-1) 263 CCAGT DraII 298 GGGGCCT [GA]G^G[ACGT]CC[CT] 419 AGGACCT EcoO109I 298 GGGGCCT [GA]G^G[ACGT]CC[CT] 419 AGGACCT HinfI 143 GAATC G^A[ACGT]TC 432 GACTC MaeIII 101 GTCAC ^GT[ACGT]AC 266 GTGAC MboII 429 GAAGA GAAGA(8/7) 146 TCTTC MspA1I 49 CAGCTG C[AC]G^C[GT]G 181 CCGCTG NlaIV 298 GGGGCC GG[ACGT]^[ACGT]CC 328 GGCACC PstI 303 CTGCAG CTGCA^G 424 CTGCAG RcaI 314 TCATGA T^CATGA 377 TCATGA Tsp45I 101 GTCAC ^GT[GC]AC 266 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AfaI AlwNI AvaII BbvI BssKI BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI HaeIII ItaI MnlI MvaI NlaIII RsaI Sau96I ScrFI SfcI TseI