(Circular) (Six-base) MAPSORT of: pFN21AE5137.seq Check: 4220 from: 1 to: 5143 pFN21AE5137 5143 bp With 182 enzymes: SgfI * January 30, 2014 17:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2953 278 Size: 53 83 186 936 1417 2468 Fragments arranged by size: 2468 1417 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2486 1937 Size: 549 4594 Acc65I G'GTAC_C Cuts at: 2464 2464 Size: 5143 AclI AA'CG_TT Cuts at: 1563 3270 3643 1563 Size: 1707 373 3063 Fragments arranged by size: 3063 1707 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2054 3206 4590 4858 1632 Size: 422 1152 1384 268 1917 Fragments arranged by size: 1917 1384 1152 422 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4946 AflIII A'CryG_T Cuts at: 1203 4042 5019 1203 Size: 2839 977 1327 Fragments arranged by size: 2839 1327 977 AgeI A'CCGG_T Cuts at: 3974 3974 Size: 5143 AhdI GACnn_n'nnGTC Cuts at: 3872 3872 Size: 5143 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5143 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5143 AlwNI CAG_nnn'CTG Cuts at: 2056 2059 2398 2886 4458 2056 Size: 3 339 488 1572 2741 Fragments arranged by size: 2741 1572 488 339 3 ApaLI G'TGCA_C Cuts at: 3200 4356 3200 Size: 1156 3987 ApoI r'AATT_y Cuts at: 1493 2452 2678 2830 4939 1493 Size: 959 226 152 2109 1697 Fragments arranged by size: 2109 1697 959 226 152 AseI AT'TA_AT Cuts at: 160 1032 3697 160 Size: 872 2665 1606 Fragments arranged by size: 2665 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5143 AvaI C'yCGr_G Cuts at: 1870 1943 2468 1870 Size: 73 525 4545 Fragments arranged by size: 4545 525 73 BamHI G'GATC_C Cuts at: 2473 2840 2473 Size: 367 4776 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2333 2464 3919 618 Size: 325 217 627 546 131 1455 1842 Fragments arranged by size: 1842 1455 627 546 325 217 131 BanII G_rGCy'C Cuts at: 1391 2462 1391 Size: 1071 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2441 928 Size: 971 542 3630 Fragments arranged by size: 3630 971 542 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3334 3368 1903 Size: 34 1397 34 3678 Fragments arranged by size: 3678 1397 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3035 4245 3035 Size: 1210 3933 BclI T'GATC_A Cuts at: 1548 1548 Size: 5143 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3754 136 Size: 107 122 71 631 605 2082 1525 Fragments arranged by size: 2082 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5138 5138 Size: 5143 BlpI GC'TnA_GC Cuts at: 2560 2560 Size: 5143 Bme1580I G_kGCm'C Cuts at: 1790 3204 4360 1790 Size: 1414 1156 2573 Fragments arranged by size: 2573 1414 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5143 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2874 3832 442 Size: 416 53 83 399 24 1457 958 1753 Fragments arranged by size: 1753 1457 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5143 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3803 1239 Size: 2564 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3268 4133 4431 3268 Size: 865 298 3980 Fragments arranged by size: 3980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3806 882 Size: 617 2307 2219 Fragments arranged by size: 2307 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2373 2934 493 Size: 711 1169 561 2702 Fragments arranged by size: 2702 1169 711 561 BsaBI GATnn'nnATC Cuts at: 5137 5137 Size: 5143 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2950 3332 3967 275 Size: 53 83 186 936 1417 382 635 1451 Fragments arranged by size: 1451 1417 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3576 3974 4248 4395 4806 3576 Size: 398 274 147 411 3913 Fragments arranged by size: 3913 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4918 Fragments arranged by size: 4918 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2398 1186 Size: 1212 3931 BseYI C'CCAG_C Cuts at: 2188 4346 4842 2188 Size: 2158 496 2489 Fragments arranged by size: 2489 2158 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2011 1163 Size: 573 275 4295 Fragments arranged by size: 4295 573 275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2593 3354 4382 1540 Size: 275 186 592 761 1028 2301 Fragments arranged by size: 2301 1028 761 592 275 186 BsiHKAI G_wGCw'C Cuts at: 3204 3289 4360 3204 Size: 85 1071 3987 Fragments arranged by size: 3987 1071 85 BsmI GAATG_Cn' Cuts at: 2654 2747 2654 Size: 93 5050 BsmBI CGTCTCn'nnnn_ Cuts at: 2233 2233 Size: 5143 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2462 3204 3289 4360 1391 Size: 399 672 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 672 399 85 BspEI T'CCGG_A Cuts at: 4806 4806 Size: 5143 BspHI T'CATG_A Cuts at: 2319 2382 3032 4762 2319 Size: 63 650 1730 2700 Fragments arranged by size: 2700 1730 650 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2436 2498 844 Size: 1592 62 3489 Fragments arranged by size: 3489 1592 62 BsrBI CCG'CTC Cuts at: 1394 1823 3030 1394 Size: 429 1207 3507 Fragments arranged by size: 3507 1207 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3638 3812 65 Size: 1183 2390 174 1396 Fragments arranged by size: 2390 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3787 3974 1700 Size: 110 1977 187 2869 Fragments arranged by size: 2869 1977 187 110 BsrGI T'GTAC_A Cuts at: 96 2349 96 Size: 2253 2890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5143 BssSI C'ACGA_G Cuts at: 3203 4215 3203 Size: 1012 4131 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5143 BstBI TT'CG_AA Cuts at: 3947 3947 Size: 5143 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5143 BstYI r'GATC_y Cuts at: 1972 2473 2840 3225 3242 4694 4780 4792 5138 Size: 501 367 385 17 1452 86 12 346 Cuts at: 5138 1972 Size: 1977 Fragments arranged by size: 1977 1452 501 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2421 2421 Size: 5143 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3652 Fragments arranged by size: 3652 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4404 Fragments arranged by size: 4404 683 56 BtsI GCAGTG_nn' Cuts at: 2659 3453 3473 2659 Size: 794 20 4329 Fragments arranged by size: 4329 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5143 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5108 DraI TTT'AAA Cuts at: 2447 2447 Size: 5143 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4150 809 Size: 507 2834 1802 Fragments arranged by size: 2834 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2590 3479 5108 Size: 54 1425 50 275 18 760 889 1629 Cuts at: 5108 8 Size: 43 Fragments arranged by size: 1629 1425 889 760 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2590 1537 Size: 275 778 4090 Fragments arranged by size: 4090 778 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3073 1353 Size: 1720 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3730 4104 4250 387 Size: 1064 138 2141 374 146 1280 Fragments arranged by size: 2141 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 3206 3803 4590 4858 1239 Size: 393 422 1152 597 787 268 1524 Fragments arranged by size: 1524 1152 787 597 422 393 268 EcoO109I rG'GnC_Cy Cuts at: 2304 2425 2304 Size: 121 5022 EcoRI G'AATT_C Cuts at: 2452 2830 2452 Size: 378 4765 FspI TGC'GCA Cuts at: 3649 3649 Size: 5143 HaeII r_GCGC'y Cuts at: 1678 4290 1678 Size: 2612 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 3784 3816 3858 Size: 32 135 32 704 32 1476 32 42 Cuts at: 3858 3890 1373 Size: 32 2626 Fragments arranged by size: 2626 1476 704 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2487 2733 669 Size: 988 281 549 246 3079 Fragments arranged by size: 3079 988 549 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5143 HpaI GTT'AAC Cuts at: 2733 2733 Size: 5143 KpnI G_GTAC'C Cuts at: 2468 2468 Size: 5143 MfeI C'AATT_G Cuts at: 2256 2742 2256 Size: 486 4657 MluI A'CGCG_T Cuts at: 5019 5019 Size: 5143 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4257 4441 1305 Size: 2952 184 2007 Fragments arranged by size: 2952 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3321 Fragments arranged by size: 3321 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3101 3460 3619 518 Size: 648 1935 359 159 2042 Fragments arranged by size: 2042 1935 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2188 2559 3236 4384 4629 1606 Size: 450 132 371 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 450 371 245 132 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3652 Fragments arranged by size: 3652 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5143 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5143 NotI GC'GGCC_GC Cuts at: 2590 2590 Size: 5143 NspI r_CATG'y Cuts at: 1132 1229 2278 2501 4046 1132 Size: 97 1049 223 1545 2229 Fragments arranged by size: 2229 1545 1049 223 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5143 PciI A'CATG_T Cuts at: 4042 4042 Size: 5143 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5143 PfoI T'CCnGG_A Cuts at: 2391 2391 Size: 5143 PmeI GTTT'AAAC Cuts at: 2447 2447 Size: 5143 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3212 3244 4725 4757 3212 Size: 32 1481 32 3598 Fragments arranged by size: 3598 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2425 2425 Size: 5143 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5143 PsiI TTA'TAA Cuts at: 2713 2713 Size: 5143 PstI C_TGCA'G Cuts at: 830 2312 2433 2495 830 Size: 1482 121 62 3478 Fragments arranged by size: 3478 1482 121 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5143 PvuII CAG'CTG Cuts at: 2056 2056 Size: 5143 RsrII CG'GwC_CG Cuts at: 2208 2208 Size: 5143 SalI G'TCGA_C Cuts at: 1936 2485 1936 Size: 549 4594 SbfI CC_TGCA'GG Cuts at: 2312 2495 2312 Size: 183 4960 ScaI AGT'ACT Cuts at: 3391 5103 3391 Size: 1712 3431 SfcI C'TryA_G Cuts at: 826 1045 2101 2308 2429 2491 3626 4307 4498 Size: 219 1056 207 121 62 1135 681 191 Cuts at: 4498 826 Size: 1471 Fragments arranged by size: 1471 1135 1056 681 219 207 191 121 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5143 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5143 SmaI CCC'GGG Cuts at: 1872 2470 1872 Size: 598 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3247 4148 4410 820 Size: 197 926 1304 901 262 1553 Fragments arranged by size: 1553 1304 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2373 2934 493 Size: 1880 561 2702 Fragments arranged by size: 2702 1880 561 SpeI A'CTAG_T Cuts at: 152 152 Size: 5143 SphI G_CATG'C Cuts at: 1132 2501 1132 Size: 1369 3774 SspI AAT'ATT Cuts at: 5 52 3067 5 Size: 47 3015 2081 Fragments arranged by size: 3015 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5143 StyI C'CwwG_G Cuts at: 513 1065 2004 2582 513 Size: 552 939 578 3074 Fragments arranged by size: 3074 939 578 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2150 3170 3187 3340 3525 3970 325 Size: 1255 570 1020 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1020 570 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 3389 5101 Size: 274 80 33 51 1443 372 1040 1712 Cuts at: 5101 96 Size: 138 Fragments arranged by size: 1712 1443 1040 372 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5103 5135 5103 Size: 32 5111 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5143 XbaI T'CTAG_A Cuts at: 2479 2479 Size: 5143 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5143 XmaI C'CCGG_G Cuts at: 1870 2468 1870 Size: 598 4545 XmnI GAAnn'nnTTC Cuts at: 3272 3272 Size: 5143 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2951 276 Size: 53 83 186 936 1417 2468 Fragments arranged by size: 2468 1417 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI