################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AviII AvrII BalI BanII BbeI BbrPI BbvCI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstBI BstUI BstXI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SphI Sse8387I SspI StuI StyI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### Acc65I 1118 GGTACC G^GTACC AlwNI 981 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 526 CCTAAGG CC^T[ACGT]AGG Asp718I 1118 GGTACC G^GTACC AspI 828 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 30 CCCGGG C^[CT]CG[GA]G BamHI 591 GGATCC G^GATCC BanI 1118 GGTACC G^G[CT][GA]CC BbsI 459 GTCTTC GAAGAC(2/6) BcgI 578 CGAGTGAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 920 GTATCC GTATCC(6/5) BpmI 217 CTCCAG CTGGAG(16/14) BpuAI 459 GTCTTC GAAGAC(2/6) BsaAI 1087 TACGTA [CT]AC^GT[GA] BsmBI 833 CGTCTC CGTCTC(1/5) BsoBI 30 CCCGGG C^[CT]CG[GA]G Bsp1407I 245 TGTACA T^GTACA BspHI 408 TCATGA T^CATGA BsrGI 245 TGTACA T^GTACA BssSI 839 CACGAG CACGAG(-5/-1) BstAPI 17 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 550 GGTGACC G^GT[ACGT]ACC BstF5I 546 GGATG GGATG(2/0) BstPI 550 GGTGACC G^GT[ACGT]ACC Bsu36I 526 CCTAAGG CC^T[ACGT]AGG DraII 399 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 526 CCTAAGG CC^T[ACGT]AGG EcoO109I 399 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 550 GGTGACC G^GT[ACGT]ACC EcoT22I 618 ATGCAT ATGCA^T FokI 546 GGATG GGATG(9/13) HaeIII 57 GGCC GG^CC HapII 31 CCGG C^CGG HgaI 983 GACGC GACGC(5/10) HpaII 31 CCGG C^CGG KpnI 1118 GGTACC GGTAC^C MseI 643 TTAA T^TAA MslI 864 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 31 CCGG C^CGG NsiI 618 ATGCAT ATGCA^T NspI 711 GCATGT [GA]CATG^[CT] PflFI 828 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 399 AGGACCT [GA]G^G[AT]CC[CT] PshAI 412 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 441 CTGCAG CTGCA^G RcaI 408 TCATGA T^CATGA SmaI 30 CCCGGG CCC^GGG SnaBI 1087 TACGTA TAC^GTA SpeI 646 ACTAGT A^CTAGT SspBI 245 TGTACA T^GTACA Tru9I 643 TTAA T^TAA Tth111I 828 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 942 CCAAGTAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 30 CCCGGG C^CCGGG XmaI 30 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AciI 15 CCGC CCGC(-3/-1) 1053 GCGG AfaI 246 GTAC GT^AC 1119 GTAC AvaII 400 GGACC G^G[AT]CC 827 GGACC BcnI 30 CCCGG CC^[GC]GG 31 CCGGG BfaI 187 CTAG C^TAG 647 CTAG BsaJI 30 CCCGGG C^C[ACGT][ACGT]GG 53 CCTCGG BsmFI 73 GGGAC GGGAC(10/14) 826 GGGAC BsrI 432 ACTGG ACTGG(1/-1) 565 ACTGG BstNI 219 CCAGG CC^[AT]GG 924 CCAGG BstYI 162 GGATCT [GA]^GATC[CT] 591 GGATCC Cfr13I 400 GGACC G^G[ACGT]CC 827 GGACC EarI 532 GAAGAG CTCTTC(1/4) 706 GAAGAG EcoRI 450 GAATTC G^AATTC 1056 GAATTC EcoRII 219 CCAGG ^CC[AT]GG 924 CCAGG HphI 550 GGTGA GGTGA(8/7) 12 TCACC Ksp632I 532 GAAGAG CTCTTC(1/4) 706 GAAGAG MaeI 187 CTAG C^TAG 647 CTAG MfeI 517 CAATTG C^AATTG 999 CAATTG MflI 162 GGATCT [GA]^GATC[CT] 591 GGATCC MspA1I 80 CAGCTG C[AC]G^C[GT]G 1044 CAGCTG MunI 517 CAATTG C^AATTG 999 CAATTG MvaI 219 CCAGG CC^[AT]GG 924 CCAGG NciI 30 CCCGG CC^[GC]GG 31 CCGGG PleI 333 GACTC GAGTC(4/5) 251 GAGTC PvuII 80 CAGCTG CAG^CTG 1044 CAGCTG RsaI 246 GTAC GT^AC 1119 GTAC SapI 532 GAAGAGC GCTCTTC(1/4) 706 GAAGAGC Sau96I 400 GGACC G^G[ACGT]CC 827 GGACC SfcI 77 CTACAG C^T[GA][CT]AG 441 CTGCAG TaqI 448 TCGA T^CGA 577 TCGA TfiI 559 GAATC G^A[AT]TC 913 GAATC TthHB8I 448 TCGA T^CGA 577 TCGA XhoII 162 GGATCT [GA]^GATC[CT] 591 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AcsI AluI AlwI ApoI BbvI BsiYI BslI BsmAI BssKI DdeI DpnII Fnu4HI HinfI ItaI MaeII MaeIII MboI MboII MnlI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI TseI Tsp45I Tsp509I TspRI