(Circular) (Six-base) MAPSORT of: pFN21AE3163.seq Check: 1243 from: 1 to: 5848 pFN21AE3163 5848 bp With 182 enzymes: SgfI * March 28, 2012 12:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 3123 3169 3123 Size: 46 5802 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 3911 5295 5563 1632 Size: 1280 999 1384 268 1917 Fragments arranged by size: 1917 1384 1280 999 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 4747 5724 1203 Size: 3544 977 1327 Fragments arranged by size: 3544 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2712 1184 Size: 1528 4320 AlwNI CAG_nnn'CTG Cuts at: 2991 3591 5163 2991 Size: 600 1572 3676 Fragments arranged by size: 3676 1572 600 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2158 2398 2455 2800 2849 3061 3157 3383 Size: 665 240 57 345 49 212 96 226 Cuts at: 3383 3535 5644 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 665 345 240 226 212 152 96 57 49 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 2035 3173 1870 Size: 73 92 1138 4545 Fragments arranged by size: 4545 1138 92 73 BamHI G'GATC_C Cuts at: 2596 3178 3545 2596 Size: 582 367 4899 Fragments arranged by size: 4899 582 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3123 3169 4624 618 Size: 325 217 627 1336 46 1455 1842 Fragments arranged by size: 1842 1455 1336 627 325 217 46 BanII G_rGCy'C Cuts at: 1391 3167 1391 Size: 1776 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2457 928 Size: 971 558 4319 Fragments arranged by size: 4319 971 558 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2572 2606 4039 4073 1903 Size: 34 635 34 1433 34 3678 Fragments arranged by size: 3678 1433 635 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2936 3740 4950 2936 Size: 804 1210 3834 Fragments arranged by size: 3834 1210 804 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BfrBI ATG'CAT Cuts at: 2625 2625 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4459 136 Size: 107 122 71 631 605 2787 1525 Fragments arranged by size: 2787 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 3909 5065 1790 Size: 2119 1156 2573 Fragments arranged by size: 2573 2119 1156 BmgBI CAC'GTC Cuts at: 1318 2838 1318 Size: 1520 4328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2447 3579 4537 Size: 416 53 83 399 24 1030 1132 958 Cuts at: 4537 442 Size: 1753 Fragments arranged by size: 1753 1132 1030 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 4508 1239 Size: 968 2301 2579 Fragments arranged by size: 2579 2301 968 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3973 4838 5136 3973 Size: 865 298 4685 Fragments arranged by size: 4685 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4511 882 Size: 617 3012 2219 Fragments arranged by size: 3012 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3094 3639 493 Size: 711 1890 545 2702 Fragments arranged by size: 2702 1890 711 545 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3655 4037 4672 275 Size: 53 83 186 936 2122 382 635 1451 Fragments arranged by size: 2122 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2016 2046 2824 2854 3107 Size: 30 165 30 613 30 778 30 253 Cuts at: 3107 3137 1178 Size: 30 3889 Fragments arranged by size: 3889 778 613 253 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5848 BseYI C'CCAG_C Cuts at: 2075 3047 3051 3144 5051 5547 2075 Size: 972 4 93 1907 496 2376 Fragments arranged by size: 2376 1907 972 496 93 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3298 4059 5087 1540 Size: 275 186 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3909 3994 5065 3909 Size: 85 1071 4692 Fragments arranged by size: 4692 1071 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 BsmBI CGTCTCn'nnnn_ Cuts at: 2844 2844 Size: 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3167 3909 3994 5065 1391 Size: 399 1377 742 85 1071 2174 Fragments arranged by size: 2174 1377 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 2413 3737 5467 2413 Size: 1324 1730 2794 Fragments arranged by size: 2794 1730 1324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3203 844 Size: 2359 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3735 1394 Size: 429 1912 3507 Fragments arranged by size: 3507 1912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4492 4679 1700 Size: 110 2682 187 2869 Fragments arranged by size: 2869 2682 187 110 BsrGI T'GTAC_A Cuts at: 96 2250 96 Size: 2154 3694 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5848 BssSI C'ACGA_G Cuts at: 2844 3908 4920 2844 Size: 1064 1012 3772 Fragments arranged by size: 3772 1064 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2028 794 Size: 1234 4614 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 2555 1184 Size: 1371 4477 BstYI r'GATC_y Cuts at: 1972 2167 2596 3178 3545 3930 3947 5399 5485 Size: 195 429 582 367 385 17 1452 86 Cuts at: 5485 5497 5843 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 582 429 385 367 346 195 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2532 2532 Size: 5848 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5296 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 3125 3364 4158 4178 3125 Size: 239 794 20 4795 Fragments arranged by size: 4795 794 239 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3295 4184 5813 Size: 54 1425 50 275 18 1465 889 1629 Cuts at: 5813 8 Size: 43 Fragments arranged by size: 1629 1465 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3295 1537 Size: 275 1483 4090 Fragments arranged by size: 4090 1483 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2532 2706 3778 1353 Size: 1179 174 1072 3423 Fragments arranged by size: 3423 1179 1072 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2912 3911 4508 5295 5563 1239 Size: 393 575 705 999 597 787 268 1524 Fragments arranged by size: 1524 999 787 705 597 575 393 268 EcoO109I rG'GnC_Cy Cuts at: 2405 2405 Size: 5848 EcoRI G'AATT_C Cuts at: 2455 3061 3157 3535 2455 Size: 606 96 378 4768 Fragments arranged by size: 4768 606 378 96 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 4995 1678 Size: 3317 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 2549 2581 2824 Size: 32 135 32 427 32 518 32 243 Cuts at: 2824 2856 4489 4521 4563 4595 1373 Size: 32 1633 32 42 32 2626 Fragments arranged by size: 2626 1633 518 427 243 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KpnI G_GTAC'C Cuts at: 3127 3173 3127 Size: 46 5802 MfeI C'AATT_G Cuts at: 2522 3004 3447 2522 Size: 482 443 4923 Fragments arranged by size: 4923 482 443 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5146 1305 Size: 3657 184 2007 Fragments arranged by size: 3657 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4026 Fragments arranged by size: 4026 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2873 3806 4165 4324 518 Size: 648 1707 933 359 159 2042 Fragments arranged by size: 2042 1707 933 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 3051 3264 3941 5089 5334 1606 Size: 481 964 213 677 1148 245 2120 Fragments arranged by size: 2120 1148 964 677 481 245 213 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5296 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NsiI A_TGCA'T Cuts at: 2627 2627 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 2720 3206 4751 1132 Size: 97 1491 486 1545 2229 Fragments arranged by size: 2229 1545 1491 486 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PfoI T'CCnGG_A Cuts at: 2223 2928 2223 Size: 705 5143 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2405 2405 Size: 5848 PshAI GACnn'nnGTC Cuts at: 1613 2421 1613 Size: 808 5040 PsiI TTA'TAA Cuts at: 2331 3418 2331 Size: 1087 4761 PstI C_TGCA'G Cuts at: 830 2450 3200 830 Size: 1620 750 3478 Fragments arranged by size: 3478 1620 750 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2087 3051 2087 Size: 964 4884 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SapI GCTCTTCn'nnn_ Cuts at: 2532 2706 2532 Size: 174 5674 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SfcI C'TryA_G Cuts at: 826 1045 2082 2446 3196 4331 5012 5203 826 Size: 219 1037 364 750 1135 681 191 1471 Fragments arranged by size: 1471 1135 1037 750 681 364 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 2037 3175 1872 Size: 165 1138 4545 Fragments arranged by size: 4545 1138 165 SmlI C'TyrA_G Cuts at: 820 1017 1943 3952 4853 5115 820 Size: 197 926 2009 901 262 1553 Fragments arranged by size: 2009 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3094 3639 493 Size: 2601 545 2702 Fragments arranged by size: 2702 2601 545 SpeI A'CTAG_T Cuts at: 152 2651 152 Size: 2499 3349 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 3287 513 Size: 552 2222 3074 Fragments arranged by size: 3074 2222 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2623 3043 3127 3875 3892 4045 4230 Size: 1255 1043 420 84 748 17 153 185 Cuts at: 4230 4675 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1043 748 445 420 185 153 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2250 4094 5806 Size: 274 80 33 51 1443 273 1844 1712 Cuts at: 5806 96 Size: 138 Fragments arranged by size: 1844 1712 1443 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5808 5840 5808 Size: 32 5816 Tth111I GACn'n_nGTC Cuts at: 1316 2836 1316 Size: 1520 4328 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2954 2954 Size: 5848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 2035 3173 1870 Size: 165 1138 4545 Fragments arranged by size: 4545 1138 165 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI