(Circular) (Six-base) MAPSORT of: pFN21AB8518.seq Check: 1309 from: 1 to: 7312 pFN21AB8518 7312 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4117 4117 Size: 7312 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3407 5122 278 Size: 53 83 186 936 1871 1715 2468 Fragments arranged by size: 2468 1871 1715 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4655 1937 Size: 2718 4594 Acc65I G'GTAC_C Cuts at: 4633 4633 Size: 7312 AclI AA'CG_TT Cuts at: 1563 5439 5812 1563 Size: 3876 373 3063 Fragments arranged by size: 3876 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2041 5375 6759 7027 1632 Size: 409 3334 1384 268 1917 Fragments arranged by size: 3334 1917 1384 409 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7115 AflIII A'CryG_T Cuts at: 1203 6211 7188 1203 Size: 5008 977 1327 Fragments arranged by size: 5008 1327 977 AgeI A'CCGG_T Cuts at: 6143 6143 Size: 7312 AhdI GACnn_n'nnGTC Cuts at: 6041 6041 Size: 7312 AleI CACnn'nnGTG Cuts at: 1166 3818 1166 Size: 2652 4660 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7312 AlwNI CAG_nnn'CTG Cuts at: 3113 4194 4352 5055 6627 3113 Size: 1081 158 703 1572 3798 Fragments arranged by size: 3798 1572 1081 703 158 ApaI G_GGCC'C Cuts at: 4086 4086 Size: 7312 ApaLI G'TGCA_C Cuts at: 5369 6525 5369 Size: 1156 6156 ApoI r'AATT_y Cuts at: 1493 2199 2494 3899 4566 4621 4847 4999 7108 Size: 706 295 1405 667 55 226 152 2109 Cuts at: 7108 1493 Size: 1697 Fragments arranged by size: 2109 1697 1405 706 667 295 226 152 55 AseI AT'TA_AT Cuts at: 160 1032 5866 160 Size: 872 4834 1606 Fragments arranged by size: 4834 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7312 AvaI C'yCGr_G Cuts at: 1870 1943 2591 4637 1870 Size: 73 648 2046 4545 Fragments arranged by size: 4545 2046 648 73 BamHI G'GATC_C Cuts at: 2643 4642 5009 2643 Size: 1999 367 4946 Fragments arranged by size: 4946 1999 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3784 4633 6088 618 Size: 325 217 627 1997 849 1455 1842 Fragments arranged by size: 1997 1842 1455 849 627 325 217 BanII G_rGCy'C Cuts at: 1391 2016 2347 2656 3095 3216 3633 4066 4086 Size: 625 331 309 439 121 417 433 20 Cuts at: 4086 4631 1391 Size: 545 4072 Fragments arranged by size: 4072 625 545 439 433 417 331 309 121 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2280 3257 928 Size: 971 381 977 4983 Fragments arranged by size: 4983 977 971 381 BbvCI CC'TCA_GC Cuts at: 3114 3114 Size: 7312 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5503 5537 1903 Size: 34 3566 34 3678 Fragments arranged by size: 3678 3566 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3723 5204 6414 3723 Size: 1481 1210 4621 Fragments arranged by size: 4621 1481 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7312 BfrBI ATG'CAT Cuts at: 2850 3283 3325 2850 Size: 433 42 6837 Fragments arranged by size: 6837 433 42 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3893 4203 5923 Size: 107 122 71 631 605 2221 310 1720 Cuts at: 5923 136 Size: 1525 Fragments arranged by size: 2221 1720 1525 631 605 310 122 107 71 BglII A'GATC_T Cuts at: 4386 7307 4386 Size: 2921 4391 BlpI GC'TnA_GC Cuts at: 3701 4104 4729 3701 Size: 403 625 6284 Fragments arranged by size: 6284 625 403 Bme1580I G_kGCm'C Cuts at: 1790 3787 4086 5373 6529 1790 Size: 1997 299 1287 1156 2573 Fragments arranged by size: 2573 1997 1287 1156 299 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7312 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2953 3089 4539 Size: 416 53 83 399 24 1536 136 1450 Cuts at: 4539 5043 6001 442 Size: 504 958 1753 Fragments arranged by size: 1753 1536 1450 958 504 416 399 136 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7312 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4408 4408 Size: 7312 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3177 3728 4057 4228 5972 1239 Size: 1938 551 329 171 1744 2579 Fragments arranged by size: 2579 1938 1744 551 329 171 Bpu10I CC'TnA_GC Cuts at: 3114 4065 3114 Size: 951 6361 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2548 2791 5437 6302 6600 2548 Size: 243 2646 865 298 3260 Fragments arranged by size: 3260 2646 865 298 243 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4075 5975 882 Size: 617 2576 1900 2219 Fragments arranged by size: 2576 2219 1900 617 BsaAI yAC'GTr Cuts at: 493 1204 4447 5103 493 Size: 711 3243 656 2702 Fragments arranged by size: 3243 2702 711 656 BsaBI GATnn'nnATC Cuts at: 7306 7306 Size: 7312 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3404 5119 5501 6136 Size: 53 83 186 936 1871 1715 382 635 Cuts at: 6136 275 Size: 1451 Fragments arranged by size: 1871 1715 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5745 6143 6417 6564 6975 5745 Size: 398 274 147 411 6082 Fragments arranged by size: 6082 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2313 2343 2365 2395 4451 Size: 30 165 30 910 30 22 30 2056 Cuts at: 4451 4481 1178 Size: 30 4009 Fragments arranged by size: 4009 2056 910 165 30 30 30 30 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2091 2243 2337 3053 3436 3439 4445 1186 Size: 905 152 94 716 383 3 1006 4053 Fragments arranged by size: 4053 1006 905 716 383 152 94 3 BseYI C'CCAG_C Cuts at: 2169 3317 6515 7011 2169 Size: 1148 3198 496 2470 Fragments arranged by size: 3198 2470 1148 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2172 2284 2681 3169 4051 1163 Size: 573 436 112 397 488 882 4424 Fragments arranged by size: 4424 882 573 488 436 397 112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4762 5523 6551 1540 Size: 275 186 2761 761 1028 2301 Fragments arranged by size: 2761 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 3216 3633 5373 5458 6529 2016 Size: 1200 417 1740 85 1071 2799 Fragments arranged by size: 2799 1740 1200 1071 417 85 BsmI GAATG_Cn' Cuts at: 2715 3308 4823 4916 2715 Size: 593 1515 93 5111 Fragments arranged by size: 5111 1515 593 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2347 2656 3095 3216 3633 3787 Size: 399 226 331 309 439 121 417 154 Cuts at: 3787 4066 4086 4631 5373 5458 6529 1391 Size: 279 20 545 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 545 439 417 399 331 309 279 226 154 121 85 20 BspEI T'CCGG_A Cuts at: 6975 6975 Size: 7312 BspHI T'CATG_A Cuts at: 5201 6931 5201 Size: 1730 5582 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4117 4667 844 Size: 3273 550 3489 Fragments arranged by size: 3489 3273 550 BsrBI CCG'CTC Cuts at: 1394 1823 3679 5199 1394 Size: 429 1856 1520 3507 Fragments arranged by size: 3507 1856 1520 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4116 5807 5981 65 Size: 1183 2868 1691 174 1396 Fragments arranged by size: 2868 1691 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3102 5956 6143 1700 Size: 110 1292 2854 187 2869 Fragments arranged by size: 2869 2854 1292 187 110 BsrGI T'GTAC_A Cuts at: 96 2702 4497 96 Size: 2606 1795 2911 Fragments arranged by size: 2911 2606 1795 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7312 BssSI C'ACGA_G Cuts at: 5372 6384 5372 Size: 1012 6300 BstAPI GCAn_nnn'nTGC Cuts at: 794 2295 2999 794 Size: 1501 704 5107 Fragments arranged by size: 5107 1501 704 BstBI TT'CG_AA Cuts at: 6116 6116 Size: 7312 BstEII G'GTnAC_C Cuts at: 1184 3986 1184 Size: 2802 4510 BstXI CCAn_nnnn'nTGG Cuts at: 4129 4129 Size: 7312 BstYI r'GATC_y Cuts at: 1972 2643 3272 4386 4642 5009 5394 5411 6863 Size: 671 629 1114 256 367 385 17 1452 Cuts at: 6863 6949 6961 7307 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1114 671 629 385 367 346 256 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3036 3036 Size: 7312 BtgI C'CryG_G Cuts at: 513 1065 3336 4488 513 Size: 552 2271 1152 3337 Fragments arranged by size: 3337 2271 1152 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6573 Fragments arranged by size: 6573 683 56 BtsI GCAGTG_nn' Cuts at: 2160 3617 4828 5622 5642 2160 Size: 1457 1211 794 20 3830 Fragments arranged by size: 3830 1457 1211 794 20 ClaI AT'CG_AT Cuts at: 1554 2569 1554 Size: 1015 6297 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2086 2121 529 Size: 35 1522 35 5720 Fragments arranged by size: 5720 1522 35 35 DraI TTT'AAA Cuts at: 4616 4616 Size: 7312 DraIII CAC_nnn'GTG Cuts at: 4027 4029 4027 Size: 2 7310 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6319 809 Size: 507 5003 1802 Fragments arranged by size: 5003 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2304 3104 4759 Size: 54 1425 50 275 18 474 800 1655 Cuts at: 4759 5648 7277 8 Size: 889 1629 43 Fragments arranged by size: 1655 1629 1425 889 800 474 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4759 1537 Size: 275 2947 4090 Fragments arranged by size: 4090 2947 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3221 3240 3457 5242 1353 Size: 1868 19 217 1785 3423 Fragments arranged by size: 3423 1868 1785 217 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5899 6273 6419 387 Size: 1064 138 4310 374 146 1280 Fragments arranged by size: 4310 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2014 3214 3631 2014 Size: 1200 417 5695 Fragments arranged by size: 5695 1200 417 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 3177 3728 4057 4228 5375 5972 Size: 393 409 1136 551 329 171 1147 597 Cuts at: 5972 6759 7027 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1147 1136 787 597 551 409 393 329 268 171 EcoO109I rG'GnC_Cy Cuts at: 3331 4083 3331 Size: 752 6560 EcoRI G'AATT_C Cuts at: 4566 4621 4999 4566 Size: 55 378 6879 Fragments arranged by size: 6879 378 55 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3020 3232 3020 Size: 212 7100 FspI TGC'GCA Cuts at: 3223 5818 3223 Size: 2595 4717 HaeII r_GCGC'y Cuts at: 1678 6459 1678 Size: 4781 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2001 2033 5953 5985 6027 Size: 32 135 32 429 32 3920 32 42 Cuts at: 6027 6059 1373 Size: 32 2626 Fragments arranged by size: 3920 2626 429 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2020 4656 4902 669 Size: 988 281 82 2636 246 3079 Fragments arranged by size: 3079 2636 988 281 246 82 HindIII A'AGCT_T Cuts at: 748 3311 748 Size: 2563 4749 HpaI GTT'AAC Cuts at: 4902 4902 Size: 7312 KpnI G_GTAC'C Cuts at: 4637 4637 Size: 7312 MfeI C'AATT_G Cuts at: 4911 4911 Size: 7312 MluI A'CGCG_T Cuts at: 7188 7188 Size: 7312 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4286 4499 6426 6610 1305 Size: 2981 213 1927 184 2007 Fragments arranged by size: 2981 2007 1927 213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5490 Fragments arranged by size: 5490 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 3818 4217 5270 5629 5788 518 Size: 648 1224 1428 399 1053 359 159 2042 Fragments arranged by size: 2042 1428 1224 1053 648 399 359 159 MspA1I CmG'CkG Cuts at: 1606 2061 2818 3110 4136 4728 5405 6553 6798 Size: 455 757 292 1026 592 677 1148 245 Cuts at: 6798 1606 Size: 2120 Fragments arranged by size: 2120 1148 1026 757 677 592 455 292 245 NaeI GCC'GGC Cuts at: 3104 3104 Size: 7312 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6760 NdeI CA'TA_TG Cuts at: 387 387 Size: 7312 NgoMIV G'CCGG_C Cuts at: 3102 3102 Size: 7312 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7312 NotI GC'GGCC_GC Cuts at: 4759 4759 Size: 7312 NsiI A_TGCA'T Cuts at: 2852 3285 3327 2852 Size: 433 42 6837 Fragments arranged by size: 6837 433 42 NspI r_CATG'y Cuts at: 1132 1229 2850 3325 4015 4582 4670 6215 1132 Size: 97 1621 475 690 567 88 1545 2229 Fragments arranged by size: 2229 1621 1545 690 567 475 97 88 PasI CC'CwG_GG Cuts at: 1348 4079 1348 Size: 2731 4581 PciI A'CATG_T Cuts at: 6211 6211 Size: 7312 PflMI CCAn_nnn'nTGG Cuts at: 1108 2736 1108 Size: 1628 5684 PfoI T'CCnGG_A Cuts at: 2183 2235 3141 3154 4244 2183 Size: 52 906 13 1090 5251 Fragments arranged by size: 5251 1090 906 52 13 PmeI GTTT'AAAC Cuts at: 4616 4616 Size: 7312 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5381 5413 6894 6926 4241 Size: 32 1108 32 1481 32 4627 Fragments arranged by size: 4627 1481 1108 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3331 3331 Size: 7312 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7312 PsiI TTA'TAA Cuts at: 3369 4332 4882 3369 Size: 963 550 5799 Fragments arranged by size: 5799 963 550 PspOMI G'GGCC_C Cuts at: 4082 4082 Size: 7312 PstI C_TGCA'G Cuts at: 830 2817 2899 4407 4537 4664 830 Size: 1987 82 1508 130 127 3478 Fragments arranged by size: 3478 1987 1508 130 127 82 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7312 PvuII CAG'CTG Cuts at: 2818 3110 4136 2818 Size: 292 1026 5994 Fragments arranged by size: 5994 1026 292 SacI G_AGCT'C Cuts at: 2016 3216 3633 2016 Size: 1200 417 5695 Fragments arranged by size: 5695 1200 417 SalI G'TCGA_C Cuts at: 1936 4654 1936 Size: 2718 4594 SanDI GG'GwC_CC Cuts at: 3331 3331 Size: 7312 SapI GCTCTTCn'nnn_ Cuts at: 3221 3221 Size: 7312 SbfI CC_TGCA'GG Cuts at: 4664 4664 Size: 7312 ScaI AGT'ACT Cuts at: 2884 3194 5560 7272 2884 Size: 310 2366 1712 2924 Fragments arranged by size: 2924 2366 1712 310 SfcI C'TryA_G Cuts at: 826 1045 2813 2895 3268 4344 4403 4533 4660 Size: 219 1768 82 373 1076 59 130 127 Cuts at: 4660 5795 6476 6667 826 Size: 1135 681 191 1471 Fragments arranged by size: 1768 1471 1135 1076 681 373 219 191 130 127 82 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7312 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7312 SmaI CCC'GGG Cuts at: 1872 4639 1872 Size: 2767 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2527 2806 5416 6317 6579 820 Size: 197 926 584 279 2610 901 262 1553 Fragments arranged by size: 2610 1553 926 901 584 279 262 197 SnaBI TAC'GTA Cuts at: 493 5103 493 Size: 4610 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7312 SphI G_CATG'C Cuts at: 1132 3325 4670 1132 Size: 2193 1345 3774 Fragments arranged by size: 3774 2193 1345 SspI AAT'ATT Cuts at: 5 52 3522 5236 5 Size: 47 3470 1714 2081 Fragments arranged by size: 3470 2081 1714 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7312 StyI C'CwwG_G Cuts at: 513 1065 2889 3716 4751 513 Size: 552 1824 827 1035 3074 Fragments arranged by size: 3074 1824 1035 827 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2808 5339 5356 5509 5694 6139 325 Size: 1255 1228 2531 17 153 185 445 1498 Fragments arranged by size: 2531 1498 1255 1228 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2429 2702 2882 Size: 274 80 33 51 1443 452 273 180 Cuts at: 2882 2961 3192 4497 5558 7270 96 Size: 79 231 1305 1061 1712 138 Fragments arranged by size: 1712 1443 1305 1061 452 274 273 231 180 138 80 79 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2313 2345 4565 4597 7272 7304 2313 Size: 32 2220 32 2675 32 2321 Fragments arranged by size: 2675 2321 2220 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7312 XbaI T'CTAG_A Cuts at: 3361 4648 3361 Size: 1287 6025 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2314 2460 4195 2266 Size: 48 146 1735 5383 Fragments arranged by size: 5383 1735 146 48 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7312 XmaI C'CCGG_G Cuts at: 1870 4637 1870 Size: 2767 4545 XmnI GAAnn'nnTTC Cuts at: 5441 5441 Size: 7312 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3405 5120 276 Size: 53 83 186 936 1871 1715 2468 Fragments arranged by size: 2468 1871 1715 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI