################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## Acc65I AccII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspLU11I BsrBI BsrFI BsrGI BssHII BstUI BstXI CelII CfoI Cfr10I CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 211 GACGTC GACGT^C AccI 1337 GTATAC GT^[AC][GT]AC AccIII 12 TCCGGA T^CCGGA AocI 358 CCTGAGG CC^T[ACGT]AGG BalI 119 TGGCCA TGG^CCA BanII 994 GGGCTC G[GA]GC[CT]^C BglI 52 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 1931 CTGGAG CTGGAG(16/14) BsaI 836 GAGACC GGTCTC(1/5) BseAI 12 TCCGGA T^CCGGA BspDI 1241 ATCGAT AT^CGAT BspEI 12 TCCGGA T^CCGGA BspHI 1793 TCATGA T^CATGA BspMI 1230 GCAGGT ACCTGC(4/8) Bst1107I 1337 GTATAC GTA^TAC BstBI 416 TTCGAA TT^CGAA BstEII 1963 GGTTACC G^GT[ACGT]ACC BstPI 1963 GGTTACC G^GT[ACGT]ACC BstZ17I 1337 GTATAC GTA^TAC Bsu36I 358 CCTGAGG CC^T[ACGT]AGG ClaI 1241 ATCGAT AT^CGAT DraIII 145 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1114 GACGCTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 697 CCATGG C^C[GA][CT]GG Eco81I 358 CCTGAGG CC^T[ACGT]AGG EcoO65I 1963 GGTTACC G^GT[ACGT]ACC EcoRI 424 GAATTC G^AATTC FauI 990 GCGGG CCCGC(4/6) MfeI 1370 CAATTG C^AATTG MluNI 119 TGGCCA TGG^CCA MroI 12 TCCGGA T^CCGGA MscI 119 TGGCCA TGG^CCA MunI 1370 CAATTG C^AATTG NcoI 697 CCATGG C^CATGG NspI 986 GCATGC [GA]CATG^[CT] NspV 416 TTCGAA TT^CGAA PpuMI 871 GGGACCC [GA]G^G[AT]CC[CT] RcaI 1793 TCATGA T^CATGA SapI 1788 GCTCTTC GCTCTTC(1/4) ScaI 1293 AGTACT AGT^ACT SfiI 51 GGCCACCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 416 TTCGAA TT^CGAA SmlI 536 CTCAAG C^T[CT][GA]AG SphI 986 GCATGC GCATG^C SwaI 491 ATTTAAAT ATTT^AAAT Tsp45I 1084 GTCAC ^GT[GC]AC XcmI 122 CCAACAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 211 GACGTC G[GA]^CG[CT]C 231 GGCGTC Asp700I 1239 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1555 GAAGTCTTTC AvaII 243 GGACC G^G[AT]CC 872 GGACC BbsI 705 GTCTTC GAAGAC(2/6) 1352 GTCTTC BbvCI 1300 CCTCAGC CCTCAGC(-5/-2) 1573 GCTGAGG BcnI 56 CCGGG CC^[GC]GG 875 CCCGG BpuAI 705 GTCTTC GAAGAC(2/6) 1352 GTCTTC BsaHI 211 GACGTC G[GA]^CG[CT]C 231 GGCGTC BsaWI 12 TCCGGA [AT]^CCGG[AT] 162 ACCGGA BsgI 1511 CTGCAC GTGCAG(16/14) 761 GTGCAG BssSI 686 CACGAG CACGAG(-5/-1) 1742 CACGAG BstYI 114 GGATCT [GA]^GATC[CT] 603 AGATCC Cac8I 986 GCATGC GC[ACGT]^[ACGT]GC 1777 GCAGGC DraI 492 TTTAAA TTT^AAA 1608 TTTAAA DraII 871 GGGACCC [GA]G^G[ACGT]CC[CT] 1913 AGGGCCT EaeI 119 TGGCCA [CT]^GGCC[GA] 1007 CGGCCA EcoO109I 871 GGGACCC [GA]G^G[ACGT]CC[CT] 1913 AGGGCCT HgaI 1114 GACGC GACGC(5/10) 232 GCGTC Hin1I 211 GACGTC G[GA]^CG[CT]C 231 GGCGTC MaeII 212 ACGT A^CGT 470 ACGT MflI 114 GGATCT [GA]^GATC[CT] 603 AGATCC NciI 56 CCGGG CC^[GC]GG 875 CCCGG PflMI 1885 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2024 CCAAAGAATGG PleI 740 GACTC GAGTC(4/5) 2016 GACTC PstI 1688 CTGCAG CTGCA^G 1750 CTGCAG SspI 289 AATATT AAT^ATT 745 AATATT Van91I 1885 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2024 CCAAAGAATGG XhoII 114 GGATCT [GA]^GATC[CT] 603 AGATCC XmnI 1239 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1555 GAAGTCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI BbvI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstAPI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I