(Circular) (Six-base) MAPSORT of: pFN21AE5783.seq Check: 1609 from: 1 to: 6748 pFN21AE5783 6748 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3226 3226 Size: 6748 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2220 4558 278 Size: 53 83 186 936 684 2338 2468 Fragments arranged by size: 2468 2338 936 684 186 83 53 AccI GT'mk_AC Cuts at: 1937 3343 4091 1937 Size: 1406 748 4594 Fragments arranged by size: 4594 1406 748 Acc65I G'GTAC_C Cuts at: 4069 4069 Size: 6748 AclI AA'CG_TT Cuts at: 1563 4875 5248 1563 Size: 3312 373 3063 Fragments arranged by size: 3312 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 3042 3193 3246 4811 6195 6463 1632 Size: 1065 345 151 53 1565 1384 268 1917 Fragments arranged by size: 1917 1565 1384 1065 345 268 151 53 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6551 AflIII A'CryG_T Cuts at: 1203 5647 6624 1203 Size: 4444 977 1327 Fragments arranged by size: 4444 1327 977 AgeI A'CCGG_T Cuts at: 5579 5579 Size: 6748 AhdI GACnn_n'nnGTC Cuts at: 5477 5477 Size: 6748 AleI CACnn'nnGTG Cuts at: 1166 3523 1166 Size: 2357 4391 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6748 AlwNI CAG_nnn'CTG Cuts at: 3224 3241 3689 3713 3922 4491 6063 3224 Size: 17 448 24 209 569 1572 3909 Fragments arranged by size: 3909 1572 569 448 209 24 17 ApaLI G'TGCA_C Cuts at: 4805 5961 4805 Size: 1156 5592 ApoI r'AATT_y Cuts at: 1493 2429 2500 3948 4057 4283 4435 6544 1493 Size: 936 71 1448 109 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 936 226 152 109 71 AseI AT'TA_AT Cuts at: 160 1032 5302 160 Size: 872 4270 1606 Fragments arranged by size: 4270 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 AvaI C'yCGr_G Cuts at: 1870 1943 4073 1870 Size: 73 2130 4545 Fragments arranged by size: 4545 2130 73 BamHI G'GATC_C Cuts at: 4078 4445 4078 Size: 367 6381 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4069 5524 618 Size: 325 217 627 2282 1455 1842 Fragments arranged by size: 2282 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3003 4067 1391 Size: 1612 1064 4072 Fragments arranged by size: 4072 1612 1064 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2703 3350 928 Size: 971 804 647 4326 Fragments arranged by size: 4326 971 804 647 BbvCI CC'TCA_GC Cuts at: 3306 3579 3306 Size: 273 6475 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4939 4973 1903 Size: 34 3002 34 3678 Fragments arranged by size: 3678 3002 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4640 5850 4640 Size: 1210 5538 BclI T'GATC_A Cuts at: 1548 1548 Size: 6748 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2063 5359 Size: 107 122 71 631 605 337 54 3296 Cuts at: 5359 136 Size: 1525 Fragments arranged by size: 3296 1525 631 605 337 122 107 71 54 BglII A'GATC_T Cuts at: 6743 6743 Size: 6748 BlpI GC'TnA_GC Cuts at: 4165 4165 Size: 6748 Bme1580I G_kGCm'C Cuts at: 1790 2692 3321 3611 4809 5965 1790 Size: 902 629 290 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 902 629 290 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6748 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2950 3072 4479 Size: 416 53 83 399 24 1533 122 1407 Cuts at: 4479 5437 442 Size: 958 1753 Fragments arranged by size: 1753 1533 1407 958 416 399 122 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6748 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3957 5408 1239 Size: 2718 1451 2579 Fragments arranged by size: 2718 2579 1451 Bpu10I CC'TnA_GC Cuts at: 2787 3306 3579 2787 Size: 519 273 5956 Fragments arranged by size: 5956 519 273 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2526 4873 5738 6036 2526 Size: 2347 865 298 3238 Fragments arranged by size: 3238 2347 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2835 5411 882 Size: 617 1336 2576 2219 Fragments arranged by size: 2576 2219 1336 617 BsaAI yAC'GTr Cuts at: 493 1204 4539 493 Size: 711 3335 2702 Fragments arranged by size: 3335 2702 711 BsaBI GATnn'nnATC Cuts at: 6742 6742 Size: 6748 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2217 2237 4555 4937 Size: 53 83 186 936 684 20 2318 382 Cuts at: 4937 5572 275 Size: 635 1451 Fragments arranged by size: 2318 1451 936 684 635 382 186 83 53 20 BsaWI w'CCGG_w Cuts at: 2017 2167 5181 5579 5853 6000 6411 2017 Size: 150 3014 398 274 147 411 2354 Fragments arranged by size: 3014 2354 411 398 274 150 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1999 2029 1178 Size: 30 165 30 596 30 5897 Fragments arranged by size: 5897 596 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2846 3244 3596 3654 1186 Size: 1660 398 352 58 4280 Fragments arranged by size: 4280 1660 398 352 58 BseYI C'CCAG_C Cuts at: 2051 3778 3821 5951 6447 2051 Size: 1727 43 2130 496 2352 Fragments arranged by size: 2352 2130 1727 496 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2787 3501 1163 Size: 573 1051 714 4410 Fragments arranged by size: 4410 1051 714 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4198 4959 5987 1540 Size: 275 186 2197 761 1028 2301 Fragments arranged by size: 2301 2197 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2838 3218 3754 4809 4894 5965 2838 Size: 380 536 1055 85 1071 3621 Fragments arranged by size: 3621 1071 1055 536 380 85 BsmI GAATG_Cn' Cuts at: 2402 2788 3163 3918 4259 4352 2402 Size: 386 375 755 341 93 4798 Fragments arranged by size: 4798 755 386 375 341 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2692 2838 3003 3218 3321 3611 3754 Size: 399 902 146 165 215 103 290 143 Cuts at: 3754 4067 4809 4894 5965 1391 Size: 313 742 85 1071 2174 Fragments arranged by size: 2174 1071 902 742 399 313 290 215 165 146 143 103 85 BspEI T'CCGG_A Cuts at: 2017 6411 2017 Size: 4394 2354 BspHI T'CATG_A Cuts at: 3798 4637 6367 3798 Size: 839 1730 4179 Fragments arranged by size: 4179 1730 839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3226 4103 844 Size: 2382 877 3489 Fragments arranged by size: 3489 2382 877 BsrBI CCG'CTC Cuts at: 1394 1823 4635 1394 Size: 429 2812 3507 Fragments arranged by size: 3507 2812 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2290 2580 3049 5243 5417 65 Size: 1183 1042 290 469 2194 174 1396 Fragments arranged by size: 2194 1396 1183 1042 469 290 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5392 5579 1700 Size: 110 3582 187 2869 Fragments arranged by size: 3582 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6748 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6748 BssSI C'ACGA_G Cuts at: 2691 3747 4808 5820 2691 Size: 1056 1061 1012 3619 Fragments arranged by size: 3619 1061 1056 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 2356 3158 3316 794 Size: 1361 201 802 158 4226 Fragments arranged by size: 4226 1361 802 201 158 BstBI TT'CG_AA Cuts at: 2422 5552 2422 Size: 3130 3618 BstEII G'GTnAC_C Cuts at: 1184 3968 1184 Size: 2784 3964 BstYI r'GATC_y Cuts at: 1972 2119 2608 4078 4445 4830 4847 6299 6385 Size: 147 489 1470 367 385 17 1452 86 Cuts at: 6385 6397 6743 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1470 1452 489 385 367 346 147 86 17 12 BstZ17I GTA'TAC Cuts at: 3344 3344 Size: 6748 Bsu36I CC'TnA_GG Cuts at: 2364 2364 Size: 6748 BtgI C'CryG_G Cuts at: 513 1065 2702 513 Size: 552 1637 4559 Fragments arranged by size: 4559 1637 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6009 Fragments arranged by size: 6009 683 56 BtsI GCAGTG_nn' Cuts at: 2046 3752 4264 5058 5078 2046 Size: 1706 512 794 20 3716 Fragments arranged by size: 3716 1706 794 512 20 ClaI AT'CG_AT Cuts at: 1554 3247 1554 Size: 1693 5055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6713 DraI TTT'AAA Cuts at: 2499 3615 4052 2499 Size: 1116 437 5195 Fragments arranged by size: 5195 1116 437 DraIII CAC_nnn'GTG Cuts at: 2155 2155 Size: 6748 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3125 5755 809 Size: 507 1809 2630 1802 Fragments arranged by size: 2630 1809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 3012 4195 Size: 54 1425 50 275 18 294 888 1183 Cuts at: 4195 5084 6713 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1183 889 888 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4195 1537 Size: 275 2383 4090 Fragments arranged by size: 4090 2383 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2504 3212 3800 4678 1353 Size: 1151 708 588 878 3423 Fragments arranged by size: 3423 1151 878 708 588 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5335 5709 5855 387 Size: 1064 138 3746 374 146 1280 Fragments arranged by size: 3746 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2697 3042 3193 3246 3957 4811 5408 Size: 393 1065 345 151 53 711 854 597 Cuts at: 5408 6195 6463 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1065 854 787 711 597 393 345 268 151 53 EcoO109I rG'GnC_Cy Cuts at: 2877 3919 2877 Size: 1042 5706 EcoRI G'AATT_C Cuts at: 2429 4057 4435 2429 Size: 1628 378 4742 Fragments arranged by size: 4742 1628 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2661 2936 2661 Size: 275 6473 FspI TGC'GCA Cuts at: 5254 5254 Size: 6748 HaeII r_GCGC'y Cuts at: 1678 5895 1678 Size: 4217 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 3727 3759 5389 Size: 32 135 32 427 32 1696 32 1630 Cuts at: 5389 5421 5463 5495 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1696 1630 427 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4092 4338 669 Size: 988 281 2154 246 3079 Fragments arranged by size: 3079 2154 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6748 HpaI GTT'AAC Cuts at: 4338 4338 Size: 6748 KpnI G_GTAC'C Cuts at: 4073 4073 Size: 6748 MfeI C'AATT_G Cuts at: 3375 4347 3375 Size: 972 5776 MluI A'CGCG_T Cuts at: 6624 6624 Size: 6748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2157 2284 3302 5862 6046 1305 Size: 852 127 1018 2560 184 2007 Fragments arranged by size: 2560 2007 1018 852 184 127 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 10 Size: 54 1425 343 294 4632 Fragments arranged by size: 4632 1425 343 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 3523 3640 4005 4706 5065 5224 518 Size: 648 2357 117 365 701 359 159 2042 Fragments arranged by size: 2357 2042 701 648 365 359 159 117 MspA1I CmG'CkG Cuts at: 1606 2245 3020 3224 3578 3866 4164 4841 5989 Size: 639 775 204 354 288 298 677 1148 Cuts at: 5989 6234 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 775 677 639 354 298 288 245 204 NcoI C'CATG_G Cuts at: 513 1065 2702 513 Size: 552 1637 4559 Fragments arranged by size: 4559 1637 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6748 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6748 NotI GC'GGCC_GC Cuts at: 4195 4195 Size: 6748 NspI r_CATG'y Cuts at: 1132 1229 2995 4106 5651 1132 Size: 97 1766 1111 1545 2229 Fragments arranged by size: 2229 1766 1545 1111 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6748 PciI A'CATG_T Cuts at: 5647 5647 Size: 6748 PflMI CCAn_nnn'nTGG Cuts at: 1108 3896 4035 1108 Size: 2788 139 3821 Fragments arranged by size: 3821 2788 139 PfoI T'CCnGG_A Cuts at: 3387 3387 Size: 6748 PmeI GTTT'AAAC Cuts at: 4052 4052 Size: 6748 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4817 4849 6330 6362 4817 Size: 32 1481 32 5203 Fragments arranged by size: 5203 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2877 2877 Size: 6748 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6748 PsiI TTA'TAA Cuts at: 2592 4318 2592 Size: 1726 5022 PstI C_TGCA'G Cuts at: 830 3697 3759 4100 830 Size: 2867 62 341 3478 Fragments arranged by size: 3478 2867 341 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6748 PvuII CAG'CTG Cuts at: 2245 3020 3224 3578 3866 2245 Size: 775 204 354 288 5127 Fragments arranged by size: 5127 775 354 288 204 SalI G'TCGA_C Cuts at: 1936 4090 1936 Size: 2154 4594 SanDI GG'GwC_CC Cuts at: 2877 2877 Size: 6748 SapI GCTCTTCn'nnn_ Cuts at: 3800 3800 Size: 6748 SbfI CC_TGCA'GG Cuts at: 4100 4100 Size: 6748 ScaI AGT'ACT Cuts at: 3300 4996 6708 3300 Size: 1696 1712 3340 Fragments arranged by size: 3340 1712 1696 SfcI C'TryA_G Cuts at: 826 1045 2852 3693 3705 3755 3861 4096 5231 Size: 219 1807 841 12 50 106 235 1135 Cuts at: 5231 5912 6103 826 Size: 681 191 1471 Fragments arranged by size: 1807 1471 1135 841 681 235 219 191 106 50 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2063 2063 Size: 6748 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6748 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6748 SmaI CCC'GGG Cuts at: 1872 4075 1872 Size: 2203 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2541 4852 5753 6015 820 Size: 197 926 598 2311 901 262 1553 Fragments arranged by size: 2311 1553 926 901 598 262 197 SnaBI TAC'GTA Cuts at: 493 4539 493 Size: 4046 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6748 SphI G_CATG'C Cuts at: 1132 2995 4106 1132 Size: 1863 1111 3774 Fragments arranged by size: 3774 1863 1111 SspI AAT'ATT Cuts at: 5 52 2296 2752 4672 5 Size: 47 2244 456 1920 2081 Fragments arranged by size: 2244 2081 1920 456 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6748 StyI C'CwwG_G Cuts at: 513 1065 2702 2757 2967 3964 4187 513 Size: 552 1637 55 210 997 223 3074 Fragments arranged by size: 3074 1637 997 552 223 210 55 SwaI ATTT'AAAT Cuts at: 2499 2499 Size: 6748 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4775 4792 4945 5130 5575 325 Size: 1255 3195 17 153 185 445 1498 Fragments arranged by size: 3195 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2324 2383 3298 Size: 274 80 33 51 1443 347 59 915 Cuts at: 3298 4994 6706 96 Size: 1696 1712 138 Fragments arranged by size: 1712 1696 1443 915 347 274 138 80 59 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6708 6740 6708 Size: 32 6716 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6748 XbaI T'CTAG_A Cuts at: 4084 4084 Size: 6748 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2134 2134 Size: 6748 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6748 XmaI C'CCGG_G Cuts at: 1870 4073 1870 Size: 2203 4545 XmnI GAAnn'nnTTC Cuts at: 3248 3564 4877 3248 Size: 316 1313 5119 Fragments arranged by size: 5119 1313 316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2218 4556 276 Size: 53 83 186 936 684 2338 2468 Fragments arranged by size: 2468 2338 936 684 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfoI SrfI