(Linear) (Six-base) MAPSORT of: KIEE5783.seq Check: 985 from: 1 to: 2043 KIEE5783.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1221 2043 Size: 1221 822 AatII G_ACGT'C Cuts at: 0 215 2043 Size: 215 1828 AccI GT'mk_AC Cuts at: 0 1338 2043 Size: 1338 705 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 692 1037 1188 1241 2043 Size: 692 345 151 53 802 Fragments arranged by size: 802 692 345 151 53 AleI CACnn'nnGTG Cuts at: 0 1518 2043 Size: 1518 525 AlwNI CAG_nnn'CTG Cuts at: 0 1219 1236 1684 1708 1917 2043 Size: 1219 17 448 24 209 126 Fragments arranged by size: 1219 448 209 126 24 17 ApoI r'AATT_y Cuts at: 0 424 495 1943 2043 Size: 424 71 1448 100 Fragments arranged by size: 1448 424 100 71 BanII G_rGCy'C Cuts at: 0 998 2043 Size: 998 1045 BbsI GAAGACnn'nnnn_ Cuts at: 0 698 1345 2043 Size: 698 647 698 Fragments arranged by size: 698 698 647 BbvCI CC'TCA_GC Cuts at: 0 1301 1574 2043 Size: 1301 273 469 Fragments arranged by size: 1301 469 273 BglI GCCn_nnn'nGGC Cuts at: 0 58 2043 Size: 58 1985 Bme1580I G_kGCm'C Cuts at: 0 687 1316 1606 2043 Size: 687 629 290 437 Fragments arranged by size: 687 629 437 290 BmrI ACTGGGnnnn_n' Cuts at: 0 945 1067 2043 Size: 945 122 976 Fragments arranged by size: 976 945 122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1952 2043 Size: 1952 91 Bpu10I CC'TnA_GC Cuts at: 0 782 1301 1574 2043 Size: 782 519 273 469 Fragments arranged by size: 782 519 469 273 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 521 2043 Size: 521 1522 BsaI GGTCTCn'nnnn_ Cuts at: 0 830 2043 Size: 830 1213 BsaHI Gr'CG_yC Cuts at: 0 212 232 2043 Size: 212 20 1811 Fragments arranged by size: 1811 212 20 BsaWI w'CCGG_w Cuts at: 0 12 162 2043 Size: 12 150 1881 Fragments arranged by size: 1881 150 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 24 2043 Size: 24 2019 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 841 1239 1591 1649 2043 Size: 841 398 352 58 394 Fragments arranged by size: 841 398 394 352 58 BseYI C'CCAG_C Cuts at: 0 46 1773 1816 2043 Size: 46 1727 43 227 Fragments arranged by size: 1727 227 46 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 782 1496 2043 Size: 782 714 547 Fragments arranged by size: 782 714 547 BsiHKAI G_wGCw'C Cuts at: 0 833 1213 1749 2043 Size: 833 380 536 294 Fragments arranged by size: 833 536 380 294 BsmI GAATG_Cn' Cuts at: 0 397 783 1158 1913 2043 Size: 397 386 375 755 130 Fragments arranged by size: 755 397 386 375 130 Bsp1286I G_dGCh'C Cuts at: 0 687 833 998 1213 1316 1606 1749 2043 Size: 687 146 165 215 103 290 143 294 Fragments arranged by size: 687 294 290 215 165 146 143 103 BspEI T'CCGG_A Cuts at: 0 12 2043 Size: 12 2031 BspHI T'CATG_A Cuts at: 0 1793 2043 Size: 1793 250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1221 2043 Size: 1221 822 BsrDI GCAATG_nn' Cuts at: 0 285 575 1044 2043 Size: 285 290 469 999 Fragments arranged by size: 999 469 290 285 BssSI C'ACGA_G Cuts at: 0 686 1742 2043 Size: 686 1056 301 Fragments arranged by size: 1056 686 301 BstAPI GCAn_nnn'nTGC Cuts at: 0 150 351 1153 1311 2043 Size: 150 201 802 158 732 Fragments arranged by size: 802 732 201 158 150 BstBI TT'CG_AA Cuts at: 0 417 2043 Size: 417 1626 BstEII G'GTnAC_C Cuts at: 0 1963 2043 Size: 1963 80 BstYI r'GATC_y Cuts at: 0 114 603 2043 Size: 114 489 1440 Fragments arranged by size: 1440 489 114 BstZ17I GTA'TAC Cuts at: 0 1339 2043 Size: 1339 704 Bsu36I CC'TnA_GG Cuts at: 0 359 2043 Size: 359 1684 BtgI C'CryG_G Cuts at: 0 697 2043 Size: 697 1346 BtsI GCAGTG_nn' Cuts at: 0 41 1747 2043 Size: 41 1706 296 Fragments arranged by size: 1706 296 41 ClaI AT'CG_AT Cuts at: 0 1242 2043 Size: 1242 801 DraI TTT'AAA Cuts at: 0 494 1610 2043 Size: 494 1116 433 Fragments arranged by size: 1116 494 433 DraIII CAC_nnn'GTG Cuts at: 0 150 2043 Size: 150 1893 DrdI GACnn_nn'nnGTC Cuts at: 0 1120 2043 Size: 1120 923 EaeI y'GGCC_r Cuts at: 0 119 1007 2043 Size: 119 888 1036 Fragments arranged by size: 1036 888 119 EarI CTCTTCn'nnn_ Cuts at: 0 499 1207 1795 2043 Size: 499 708 588 248 Fragments arranged by size: 708 588 499 248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 692 1037 1188 1241 1952 2043 Size: 692 345 151 53 711 91 Fragments arranged by size: 711 692 345 151 91 53 EcoO109I rG'GnC_Cy Cuts at: 0 872 1914 2043 Size: 872 1042 129 Fragments arranged by size: 1042 872 129 EcoRI G'AATT_C Cuts at: 0 424 2043 Size: 424 1619 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 656 931 2043 Size: 656 275 1112 Fragments arranged by size: 1112 656 275 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 26 1722 1754 2043 Size: 26 1696 32 289 Fragments arranged by size: 1696 289 32 26 MfeI C'AATT_G Cuts at: 0 1370 2043 Size: 1370 673 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 152 279 1297 2043 Size: 152 127 1018 746 Fragments arranged by size: 1018 746 152 127 MscI TGG'CCA Cuts at: 0 121 2043 Size: 121 1922 MslI CAynn'nnrTG Cuts at: 0 1518 1635 2000 2043 Size: 1518 117 365 43 Fragments arranged by size: 1518 365 117 43 MspA1I CmG'CkG Cuts at: 0 240 1015 1219 1573 1861 2043 Size: 240 775 204 354 288 182 Fragments arranged by size: 775 354 288 240 204 182 NcoI C'CATG_G Cuts at: 0 697 2043 Size: 697 1346 NspI r_CATG'y Cuts at: 0 990 2043 Size: 990 1053 PflMI CCAn_nnn'nTGG Cuts at: 0 1891 2030 2043 Size: 1891 139 13 Fragments arranged by size: 1891 139 13 PfoI T'CCnGG_A Cuts at: 0 1382 2043 Size: 1382 661 PpuMI rG'GwC_Cy Cuts at: 0 872 2043 Size: 872 1171 PsiI TTA'TAA Cuts at: 0 587 2043 Size: 587 1456 PstI C_TGCA'G Cuts at: 0 1692 1754 2043 Size: 1692 62 289 Fragments arranged by size: 1692 289 62 PvuII CAG'CTG Cuts at: 0 240 1015 1219 1573 1861 2043 Size: 240 775 204 354 288 182 Fragments arranged by size: 775 354 288 240 204 182 SanDI GG'GwC_CC Cuts at: 0 872 2043 Size: 872 1171 SapI GCTCTTCn'nnn_ Cuts at: 0 1795 2043 Size: 1795 248 ScaI AGT'ACT Cuts at: 0 1295 2043 Size: 1295 748 SfcI C'TryA_G Cuts at: 0 847 1688 1700 1750 1856 2043 Size: 847 841 12 50 106 187 Fragments arranged by size: 847 841 187 106 50 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 58 2043 Size: 58 1985 SmlI C'TyrA_G Cuts at: 0 536 2043 Size: 536 1507 SphI G_CATG'C Cuts at: 0 990 2043 Size: 990 1053 SspI AAT'ATT Cuts at: 0 291 747 2043 Size: 291 456 1296 Fragments arranged by size: 1296 456 291 StyI C'CwwG_G Cuts at: 0 697 752 962 1959 2043 Size: 697 55 210 997 84 Fragments arranged by size: 997 697 210 84 55 SwaI ATTT'AAAT Cuts at: 0 494 2043 Size: 494 1549 TatI w'GTAC_w Cuts at: 0 319 378 1293 2043 Size: 319 59 915 750 Fragments arranged by size: 915 750 319 59 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 129 2043 Size: 129 1914 XmnI GAAnn'nnTTC Cuts at: 0 1243 1559 2043 Size: 1243 316 484 Fragments arranged by size: 1243 484 316 ZraI GAC'GTC Cuts at: 0 213 2043 Size: 213 1830 Enzymes that do cut: AarI AatII AccI AcuI AleI AlwNI ApoI BanII BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I EcoRI FalI Hin4I MfeI MmeI MscI MslI MspA1I NcoI NspI PflMI PfoI PpuMI PsiI PstI PvuII SanDI SapI ScaI SfcI SfiI SmlI SphI SspI StyI SwaI TatI XcmI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BstXI BtgZI CspCI EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI TaqII TstI Tth111I XbaI XhoI XmaI