(Linear) (Six-base) MAPSORT of: KIEE1276.seq Check: 1449 from: 1 to: 2256 KIEE1276.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. Acc65I G'GTAC_C Cuts at: 0 1648 2256 Size: 1648 608 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 106 734 1949 2110 2256 Size: 25 81 628 1215 161 146 Fragments arranged by size: 1215 628 161 146 81 25 AflIII A'CryG_T Cuts at: 0 732 917 1480 2256 Size: 732 185 563 776 Fragments arranged by size: 776 732 563 185 AhdI GACnn_n'nnGTC Cuts at: 0 1947 2256 Size: 1947 309 AleI CACnn'nnGTG Cuts at: 0 953 2256 Size: 953 1303 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1653 1685 2256 Size: 1653 32 571 Fragments arranged by size: 1653 571 32 ApoI r'AATT_y Cuts at: 0 540 857 1002 1069 1190 2043 2256 Size: 540 317 145 67 121 853 213 Fragments arranged by size: 853 540 317 213 145 121 67 AseI AT'TA_AT Cuts at: 0 1183 1978 2256 Size: 1183 795 278 Fragments arranged by size: 1183 795 278 BanI G'GyrC_C Cuts at: 0 1648 2256 Size: 1648 608 BanII G_rGCy'C Cuts at: 0 2247 2256 Size: 2247 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 1937 2170 2177 2256 Size: 1937 233 7 79 Fragments arranged by size: 1937 233 79 7 BglI GCCn_nnn'nGGC Cuts at: 0 1030 2256 Size: 1030 1226 BglII A'GATC_T Cuts at: 0 1636 2256 Size: 1636 620 BlpI GC'TnA_GC Cuts at: 0 995 2256 Size: 995 1261 BmrI ACTGGGnnnn_n' Cuts at: 0 1168 1654 2256 Size: 1168 486 602 Fragments arranged by size: 1168 602 486 Bpu10I CC'TnA_GC Cuts at: 0 1694 2256 Size: 1694 562 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 832 2256 Size: 832 1424 BsaBI GATnn'nnATC Cuts at: 0 609 779 977 2256 Size: 609 170 198 1279 Fragments arranged by size: 1279 609 198 170 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1653 1683 2256 Size: 1653 30 573 Fragments arranged by size: 1653 573 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 448 1556 2256 Size: 448 1108 700 Fragments arranged by size: 1108 700 448 BseYI C'CCAG_C Cuts at: 0 1730 2256 Size: 1730 526 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 656 2256 Size: 656 1600 BsiHKAI G_wGCw'C Cuts at: 0 950 2247 2256 Size: 950 1297 9 Fragments arranged by size: 1297 950 9 BsmI GAATG_Cn' Cuts at: 0 1222 1686 2256 Size: 1222 464 570 Fragments arranged by size: 1222 570 464 Bsp1286I G_dGCh'C Cuts at: 0 950 2247 2256 Size: 950 1297 9 Fragments arranged by size: 1297 950 9 BspHI T'CATG_A Cuts at: 0 54 2256 Size: 54 2202 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1827 2256 Size: 1827 429 BstAPI GCAn_nnn'nTGC Cuts at: 0 490 2256 Size: 490 1766 BstXI CCAn_nnnn'nTGG Cuts at: 0 326 2256 Size: 326 1930 BstYI r'GATC_y Cuts at: 0 686 780 1636 2256 Size: 686 94 856 620 Fragments arranged by size: 856 686 620 94 Bsu36I CC'TnA_GG Cuts at: 0 404 2256 Size: 404 1852 BtgI C'CryG_G Cuts at: 0 1025 1211 2256 Size: 1025 186 1045 Fragments arranged by size: 1045 1025 186 BtsI GCAGTG_nn' Cuts at: 0 24 376 2256 Size: 24 352 1880 Fragments arranged by size: 1880 352 24 ClaI AT'CG_AT Cuts at: 0 225 2256 Size: 225 2031 DraI TTT'AAA Cuts at: 0 242 350 1772 2256 Size: 242 108 1422 484 Fragments arranged by size: 1422 484 242 108 EaeI y'GGCC_r Cuts at: 0 1022 2256 Size: 1022 1234 EarI CTCTTCn'nnn_ Cuts at: 0 1 425 1342 1574 2256 Size: 1 424 917 232 682 Fragments arranged by size: 917 682 424 232 1 EcoICRI GAG'CTC Cuts at: 0 2245 2256 Size: 2245 11 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 106 734 1949 2110 2256 Size: 25 81 628 1215 161 146 Fragments arranged by size: 1215 628 161 146 81 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 1804 2256 Size: 1804 452 EcoO109I rG'GnC_Cy Cuts at: 0 262 2256 Size: 262 1994 EcoRI G'AATT_C Cuts at: 0 540 857 1002 1190 2256 Size: 540 317 145 188 1066 Fragments arranged by size: 1066 540 317 188 145 EcoRV GAT'ATC Cuts at: 0 2206 2256 Size: 2206 50 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 347 2194 2256 Size: 347 1847 62 Fragments arranged by size: 1847 347 62 HaeII r_GCGC'y Cuts at: 0 1426 2256 Size: 1426 830 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 246 278 904 936 1933 1965 2256 Size: 246 32 626 32 997 32 291 Fragments arranged by size: 997 626 291 246 32 32 32 HincII GTy'rAC Cuts at: 0 1925 2256 Size: 1925 331 KpnI G_GTAC'C Cuts at: 0 1652 2256 Size: 1652 604 MfeI C'AATT_G Cuts at: 0 720 2256 Size: 720 1536 MslI CAynn'nnrTG Cuts at: 0 59 953 2256 Size: 59 894 1303 Fragments arranged by size: 1303 894 59 MspA1I CmG'CkG Cuts at: 0 832 2256 Size: 832 1424 NcoI C'CATG_G Cuts at: 0 1211 2256 Size: 1211 1045 NdeI CA'TA_TG Cuts at: 0 650 2256 Size: 650 1606 NspI r_CATG'y Cuts at: 0 736 921 1484 1947 2256 Size: 736 185 563 463 309 Fragments arranged by size: 736 563 463 309 185 PasI CC'CwG_GG Cuts at: 0 691 2256 Size: 691 1565 PciI A'CATG_T Cuts at: 0 732 917 1480 2256 Size: 732 185 563 776 Fragments arranged by size: 776 732 563 185 PfoI T'CCnGG_A Cuts at: 0 421 504 2256 Size: 421 83 1752 Fragments arranged by size: 1752 421 83 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1653 1685 2256 Size: 1653 32 571 Fragments arranged by size: 1653 571 32 PpuMI rG'GwC_Cy Cuts at: 0 262 2256 Size: 262 1994 PvuII CAG'CTG Cuts at: 0 832 2256 Size: 832 1424 SacI G_AGCT'C Cuts at: 0 2247 2256 Size: 2247 9 ScaI AGT'ACT Cuts at: 0 2214 2235 2256 Size: 2214 21 21 Fragments arranged by size: 2214 21 21 SfcI C'TryA_G Cuts at: 0 1106 2256 Size: 1106 1150 SmlI C'TyrA_G Cuts at: 0 811 2256 Size: 811 1445 SpeI A'CTAG_T Cuts at: 0 952 1878 2256 Size: 952 926 378 Fragments arranged by size: 952 926 378 StyI C'CwwG_G Cuts at: 0 265 1211 2256 Size: 265 946 1045 Fragments arranged by size: 1045 946 265 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1023 2256 Size: 1023 1233 TatI w'GTAC_w Cuts at: 0 1239 2212 2233 2256 Size: 1239 973 21 23 Fragments arranged by size: 1239 973 23 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1319 1351 2256 Size: 1319 32 905 Fragments arranged by size: 1319 905 32 XbaI T'CTAG_A Cuts at: 0 1083 2256 Size: 1083 1173 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1017 2256 Size: 1017 1239 XmnI GAAnn'nnTTC Cuts at: 0 302 734 879 1286 1757 2256 Size: 302 432 145 407 471 499 Fragments arranged by size: 499 471 432 407 302 145 Enzymes that do cut: Acc65I AcuI AflIII AhdI AleI AloI ApoI AseI BanI BanII BbsI BglI BglII BlpI BmrI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BstAPI BstXI BstYI Bsu36I BtgI BtsI ClaI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII KpnI MfeI MslI MspA1I NcoI NdeI NspI PasI PciI PfoI PpiI PpuMI PvuII SacI ScaI SfcI SmlI SpeI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AlfI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI Bme1580I BmgBI BmtI BplI BpmI BsaI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI CspCI DraIII DrdI EagI EciI FseI FspI FspAI HindIII HpaI KasI MluI MmeI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI ZraI