################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeIII MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AlwI 191 GGATC GGATC(4/5) AlwNI 706 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 524 AGCGCT AGC^GCT Asp700I 522 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 355 GTCTTC GAAGAC(2/6) BglII 764 AGATCT A^GATCT BpmI 310 CTGGAG CTGGAG(16/14) BpuAI 355 GTCTTC GAAGAC(2/6) BsaBI 942 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 647 CGGCCG CG[GA][CT]^CG BsmI 743 GAATGC GAATGC(1/-1) BsrBI 1024 CCGCTC CCGCTC(-3/-3) BsrI 792 ACTGG ACTGG(1/-1) BssHII 608 GCGCGC G^CGCGC BstAPI 483 GCAGTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 764 AGATCT [GA]^GATC[CT] EagI 647 CGGCCG C^GGCCG EclXI 647 CGGCCG C^GGCCG Eco47III 524 AGCGCT AGC^GCT Eco52I 647 CGGCCG C^GGCCG EcoNI 309 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 57 GCGTC GACGC(5/10) HinfI 980 GACTC G^A[ACGT]TC KspI 903 CCGCGG CCGC^GG MamI 942 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 764 AGATCT [GA]^GATC[CT] PleI 980 GACTC GAGTC(4/5) PvuII 855 CAGCTG CAG^CTG SacII 903 CCGCGG CCGC^GG SnaBI 1126 TACGTA TAC^GTA XhoII 764 AGATCT [GA]^GATC[CT] XmnI 522 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### ApaI 27 GGGCCC GGGCC^C 65 GGGCCC AvaI 61 CCCGGG C^[CT]CG[GA]G 632 CCCGGG BbrPI 385 CACGTG CAC^GTG 987 CACGTG BfaI 256 CTAG C^TAG 403 CTAG BglI 428 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 677 GCCCCAGGGGC BseRI 264 CTCCTC GAGGAG(10/8) 367 CTCCTC BsgI 286 CTGCAC GTGCAG(16/14) 1120 GTGCAG BsoBI 61 CCCGGG C^[CT]CG[GA]G 632 CCCGGG BspMI 245 ACCTGC ACCTGC(4/8) 323 ACCTGC BsrFI 97 GCCGGC [GA]^CCGG[CT] 132 GCCGGC Cfr10I 97 GCCGGC [GA]^CCGG[CT] 132 GCCGGC DsaI 672 CCGTGG C^C[GA][CT]GG 903 CCGCGG EaeI 647 CGGCCG [CT]^GGCC[GA] 819 CGGCCA MaeI 256 CTAG C^TAG 403 CTAG NaeI 97 GCCGGC GCC^GGC 132 GCCGGC NgoMIV 97 GCCGGC G^CCGGC 132 GCCGGC PmaCI 385 CACGTG CAC^GTG 987 CACGTG PmlI 385 CACGTG CAC^GTG 987 CACGTG PpuMI 635 GGGTCCC [GA]G^G[AT]CC[CT] 1091 AGGACCT SmaI 61 CCCGGG CCC^GGG 632 CCCGGG TspRI 120 CACTG CA[GC]TG[ACGT][ACGT]^ 505 CACTG XmaCI 61 CCCGGG C^CCGGG 632 CCCGGG XmaI 61 CCCGGG C^CCGGG 632 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AvaII BanI BanII BbeI BbvI BcnI BmyI BsaAI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI TseI Tsp509I