(Circular) (Six-base) MAPSORT of: pFN21AE3162.seq Check: 4493 from: 1 to: 5845 pFN21AE3162 5845 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3655 278 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3188 1937 Size: 1251 4594 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3144 3972 4345 1563 Size: 1581 828 373 3063 Fragments arranged by size: 3063 1581 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 2546 3908 5292 5560 1632 Size: 519 395 1362 1384 268 1917 Fragments arranged by size: 1917 1384 1362 519 395 268 AfeI AGC'GCT Cuts at: 2531 2531 Size: 5845 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 2829 2958 3111 4744 5721 1203 Size: 1626 129 153 1633 977 1327 Fragments arranged by size: 1633 1626 1327 977 153 129 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2122 1184 Size: 938 4907 AlwNI CAG_nnn'CTG Cuts at: 2716 3588 5160 2716 Size: 872 1572 3401 Fragments arranged by size: 3401 1572 872 ApaI G_GGCC'C Cuts at: 2036 2074 2036 Size: 38 5807 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 3154 3380 3532 5641 1493 Size: 1661 226 152 2109 1697 Fragments arranged by size: 2109 1697 1661 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2066 2637 3170 1870 Size: 73 123 571 533 4545 Fragments arranged by size: 4545 571 533 123 73 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2461 2633 2690 2932 3166 Size: 325 217 627 674 172 57 242 234 Cuts at: 3166 4621 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 674 627 325 242 234 217 172 57 BanII G_rGCy'C Cuts at: 1391 2036 2074 2662 2704 3164 1391 Size: 645 38 588 42 460 4072 Fragments arranged by size: 4072 645 588 460 42 38 BbeI G_GCGC'C Cuts at: 2465 2637 2694 2465 Size: 172 57 5616 Fragments arranged by size: 5616 172 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2353 928 Size: 971 454 4420 Fragments arranged by size: 4420 971 454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2439 2688 4456 Size: 107 122 71 631 605 767 249 1768 Cuts at: 4456 136 Size: 1525 Fragments arranged by size: 1768 1525 767 631 605 249 122 107 71 BglII A'GATC_T Cuts at: 2769 5840 2769 Size: 3071 2774 BlpI GC'TnA_GC Cuts at: 3262 3262 Size: 5845 Bme1580I G_kGCm'C Cuts at: 1790 2036 2074 2921 2937 3906 5062 1790 Size: 246 38 847 16 969 1156 2573 Fragments arranged by size: 2573 1156 969 847 246 38 16 BmgBI CAC'GTC Cuts at: 1318 2020 2209 2832 1318 Size: 702 189 623 4331 Fragments arranged by size: 4331 702 623 189 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2807 3576 4534 Size: 416 53 83 399 24 1390 769 958 Cuts at: 4534 442 Size: 1753 Fragments arranged by size: 1753 1390 958 769 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 4505 1239 Size: 1097 2169 2579 Fragments arranged by size: 2579 2169 1097 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3970 4835 5133 3970 Size: 865 298 4682 Fragments arranged by size: 4682 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4508 882 Size: 617 3009 2219 Fragments arranged by size: 3009 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2392 2494 2944 2959 2994 3133 3636 Size: 711 1188 102 450 15 35 139 503 Cuts at: 3636 493 Size: 2702 Fragments arranged by size: 2702 1188 711 503 450 139 102 35 15 BsaBI GATnn'nnATC Cuts at: 2951 5839 2951 Size: 2888 2957 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2062 2462 2634 2691 Size: 53 83 186 936 529 400 172 57 Cuts at: 2691 3652 4034 4669 275 Size: 961 382 635 1451 Fragments arranged by size: 1451 961 936 635 529 400 382 186 172 83 57 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2105 2135 2309 2339 1178 Size: 30 165 30 702 30 174 30 4684 Fragments arranged by size: 4684 702 174 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2260 2363 1186 Size: 1074 103 4668 Fragments arranged by size: 4668 1074 103 BseYI C'CCAG_C Cuts at: 2705 5048 5544 2705 Size: 2343 496 3006 Fragments arranged by size: 3006 2343 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 3146 1163 Size: 573 540 870 3862 Fragments arranged by size: 3862 870 573 540 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2655 3295 4056 5084 1540 Size: 275 186 654 640 761 1028 2301 Fragments arranged by size: 2301 1028 761 654 640 275 186 BsiHKAI G_wGCw'C Cuts at: 3906 3991 5062 3906 Size: 85 1071 4689 Fragments arranged by size: 4689 1071 85 BsmI GAATG_Cn' Cuts at: 2754 3356 3449 2754 Size: 602 93 5150 Fragments arranged by size: 5150 602 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2074 2662 2704 2921 2937 3164 Size: 399 246 38 588 42 217 16 227 Cuts at: 3164 3906 3991 5062 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 588 399 246 227 217 85 42 38 16 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2259 2337 3200 844 Size: 1415 78 863 3489 Fragments arranged by size: 3489 1415 863 78 BsrBI CCG'CTC Cuts at: 1394 1823 3031 3732 1394 Size: 429 1208 701 3507 Fragments arranged by size: 3507 1208 701 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4340 4514 65 Size: 1183 3092 174 1396 Fragments arranged by size: 3092 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2102 2137 4489 4676 1700 Size: 110 292 35 2352 187 2869 Fragments arranged by size: 2869 2352 292 187 110 35 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5845 BssHII G'CGCG_C Cuts at: 1925 2613 1925 Size: 688 5157 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 2494 794 Size: 1700 4145 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstYI r'GATC_y Cuts at: 1972 2769 3175 3542 3927 3944 5396 5482 5494 Size: 797 406 367 385 17 1452 86 12 Cuts at: 5494 5840 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 797 406 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2677 2908 513 Size: 552 939 673 231 3450 Fragments arranged by size: 3450 939 673 552 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2509 3361 4155 4175 2124 Size: 385 852 794 20 3794 Fragments arranged by size: 3794 852 794 385 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2652 2824 3292 Size: 54 1425 50 275 18 822 172 468 Cuts at: 3292 4181 5810 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 822 468 275 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2652 3292 1537 Size: 275 840 640 4090 Fragments arranged by size: 4090 840 640 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3775 1353 Size: 2422 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2336 2546 3908 4505 5292 5560 Size: 393 519 185 210 1362 597 787 268 Cuts at: 5560 1239 Size: 1524 Fragments arranged by size: 1524 1362 787 597 519 393 268 210 185 EcoNI CCTnn'n_nnAGG Cuts at: 2318 2318 Size: 5845 EcoO109I rG'GnC_Cy Cuts at: 2032 2033 2070 2071 2641 3097 2032 Size: 1 37 1 570 456 4780 Fragments arranged by size: 4780 570 456 37 1 1 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 5845 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 2138 2151 2233 2344 2423 2465 2474 2486 Size: 460 13 82 111 79 42 9 12 Cuts at: 2486 2504 2533 2637 2694 4992 1678 Size: 18 29 104 57 2298 2531 Fragments arranged by size: 2531 2298 460 111 104 82 79 57 42 29 18 13 12 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 4486 4518 4560 Size: 32 135 32 533 32 2349 32 42 Cuts at: 4560 4592 1373 Size: 32 2626 Fragments arranged by size: 2626 2349 533 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3189 3435 669 Size: 988 281 1251 246 3079 Fragments arranged by size: 3079 1251 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5845 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KasI G'GCGC_C Cuts at: 2461 2633 2690 2461 Size: 172 57 5616 Fragments arranged by size: 5616 172 57 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3132 4959 5143 1305 Size: 1827 1827 184 2007 Fragments arranged by size: 2007 1827 1827 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4023 Fragments arranged by size: 4023 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2942 2957 3056 3803 4162 4321 518 Size: 648 1776 15 99 747 359 159 2042 Fragments arranged by size: 2042 1776 747 648 359 159 99 15 MspA1I CmG'CkG Cuts at: 1606 2119 2283 2338 2605 2823 2862 2910 3261 Size: 513 164 55 267 218 39 48 351 Cuts at: 3261 3938 5086 5331 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 513 351 267 245 218 164 55 48 39 NaeI GCC'GGC Cuts at: 2104 2139 2104 Size: 35 5810 NarI GG'CG_CC Cuts at: 2462 2634 2691 2462 Size: 172 57 5616 Fragments arranged by size: 5616 172 57 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4354 Fragments arranged by size: 4354 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NgoMIV G'CCGG_C Cuts at: 2102 2137 2102 Size: 35 5810 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 3203 4748 1132 Size: 97 1974 1545 2229 Fragments arranged by size: 2229 1974 1545 97 PasI CC'CwG_GG Cuts at: 1348 2685 1348 Size: 1337 4508 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5845 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PmlI CAC'GTG Cuts at: 2392 2994 2392 Size: 602 5243 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2641 3097 2641 Size: 456 5389 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspOMI G'GGCC_C Cuts at: 2032 2070 2032 Size: 38 5807 PstI C_TGCA'G Cuts at: 830 2334 2403 2490 2867 3197 830 Size: 1504 69 87 377 330 3478 Fragments arranged by size: 3478 1504 377 330 87 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2862 2862 Size: 5845 SacII CC_GC'GG Cuts at: 2911 2911 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SanDI GG'GwC_CC Cuts at: 2641 2641 Size: 5845 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 2330 2399 2486 2863 2902 3193 4328 Size: 219 1285 69 87 377 39 291 1135 Cuts at: 4328 5009 5200 826 Size: 681 191 1471 Fragments arranged by size: 1471 1285 1135 681 377 291 219 191 87 69 39 SfoI GGC'GCC Cuts at: 2463 2635 2692 2463 Size: 172 57 5616 Fragments arranged by size: 5616 172 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 2068 2639 3172 1872 Size: 196 571 533 4545 Fragments arranged by size: 4545 571 533 196 SmlI C'TyrA_G Cuts at: 820 1017 1943 3949 4850 5112 820 Size: 197 926 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3133 3636 493 Size: 2640 503 2702 Fragments arranged by size: 2702 2640 503 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 2004 3284 513 Size: 552 939 1280 3074 Fragments arranged by size: 3074 1280 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 3872 3889 4042 4227 4672 325 Size: 1255 738 1554 17 153 185 445 1498 Fragments arranged by size: 1554 1498 1255 738 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4091 5803 96 Size: 274 80 33 51 1443 2114 1712 138 Fragments arranged by size: 2114 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5805 5837 5805 Size: 32 5813 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 2066 2637 3170 1870 Size: 196 571 533 4545 Fragments arranged by size: 4545 571 533 196 XmnI GAAnn'nnTTC Cuts at: 2531 3974 2531 Size: 1443 4402 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3653 276 Size: 53 83 186 936 2119 2468 Fragments arranged by size: 2468 2119 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI