(Linear) (Six-base) MAPSORT of: KIEE3162.seq Check: 9850 from: 1 to: 1140 KIEE3162.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 541 1140 Size: 146 395 599 Fragments arranged by size: 599 395 146 AfeI AGC'GCT Cuts at: 0 526 1140 Size: 526 614 AflIII A'CryG_T Cuts at: 0 824 953 1106 1140 Size: 824 129 153 34 Fragments arranged by size: 824 153 129 34 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 117 1140 Size: 117 1023 AlwNI CAG_nnn'CTG Cuts at: 0 711 1140 Size: 711 429 ApaI G_GGCC'C Cuts at: 0 31 69 1140 Size: 31 38 1071 Fragments arranged by size: 1071 38 31 AvaI C'yCGr_G Cuts at: 0 61 632 1140 Size: 61 571 508 Fragments arranged by size: 571 508 61 BanI G'GyrC_C Cuts at: 0 456 628 685 927 1140 Size: 456 172 57 242 213 Fragments arranged by size: 456 242 213 172 57 BanII G_rGCy'C Cuts at: 0 31 69 657 699 1140 Size: 31 38 588 42 441 Fragments arranged by size: 588 441 42 38 31 BbeI G_GCGC'C Cuts at: 0 460 632 689 1140 Size: 460 172 57 451 Fragments arranged by size: 460 451 172 57 BbsI GAAGACnn'nnnn_ Cuts at: 0 348 1140 Size: 348 792 BglI GCCn_nnn'nGGC Cuts at: 0 434 683 1140 Size: 434 249 457 Fragments arranged by size: 457 434 249 BglII A'GATC_T Cuts at: 0 764 1140 Size: 764 376 Bme1580I G_kGCm'C Cuts at: 0 31 69 916 932 1140 Size: 31 38 847 16 208 Fragments arranged by size: 847 208 38 31 16 BmgBI CAC'GTC Cuts at: 0 15 204 827 1140 Size: 15 189 623 313 Fragments arranged by size: 623 313 189 15 BmrI ACTGGGnnnn_n' Cuts at: 0 802 1140 Size: 802 338 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 1140 Size: 331 809 BsaAI yAC'GTr Cuts at: 0 387 489 939 954 989 1128 1140 Size: 387 102 450 15 35 139 12 Fragments arranged by size: 450 387 139 102 35 15 12 BsaBI GATnn'nnATC Cuts at: 0 946 1140 Size: 946 194 BsaHI Gr'CG_yC Cuts at: 0 57 457 629 686 1140 Size: 57 400 172 57 454 Fragments arranged by size: 454 400 172 57 57 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 100 130 304 334 1140 Size: 100 30 174 30 806 Fragments arranged by size: 806 174 100 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 255 358 1140 Size: 255 103 782 Fragments arranged by size: 782 255 103 BseYI C'CCAG_C Cuts at: 0 700 1140 Size: 700 440 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 1140 Size: 271 869 BsiEI CG_ry'CG Cuts at: 0 650 1140 Size: 650 490 BsmI GAATG_Cn' Cuts at: 0 749 1140 Size: 749 391 Bsp1286I G_dGCh'C Cuts at: 0 31 69 657 699 916 932 1140 Size: 31 38 588 42 217 16 208 Fragments arranged by size: 588 217 208 42 38 31 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 254 332 1140 Size: 254 78 808 Fragments arranged by size: 808 254 78 BsrBI CCG'CTC Cuts at: 0 1026 1140 Size: 1026 114 BsrFI r'CCGG_y Cuts at: 0 97 132 1140 Size: 97 35 1008 Fragments arranged by size: 1008 97 35 BssHII G'CGCG_C Cuts at: 0 608 1140 Size: 608 532 BstAPI GCAn_nnn'nTGC Cuts at: 0 489 1140 Size: 489 651 BstYI r'GATC_y Cuts at: 0 764 1140 Size: 764 376 BtgI C'CryG_G Cuts at: 0 672 903 1140 Size: 672 231 237 Fragments arranged by size: 672 237 231 BtsI GCAGTG_nn' Cuts at: 0 119 504 1140 Size: 119 385 636 Fragments arranged by size: 636 385 119 EaeI y'GGCC_r Cuts at: 0 647 819 1140 Size: 647 172 321 Fragments arranged by size: 647 321 172 EagI C'GGCC_G Cuts at: 0 647 1140 Size: 647 493 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 331 541 1140 Size: 146 185 210 599 Fragments arranged by size: 599 210 185 146 EcoNI CCTnn'n_nnAGG Cuts at: 0 313 1140 Size: 313 827 EcoO109I rG'GnC_Cy Cuts at: 0 27 28 65 66 636 1092 1140 Size: 27 1 37 1 570 456 48 Fragments arranged by size: 570 456 48 37 27 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 900 1140 Size: 900 240 HaeII r_GCGC'y Cuts at: 0 133 146 228 339 418 460 469 481 Size: 133 13 82 111 79 42 9 12 Cuts at: 481 499 528 632 689 1140 Size: 18 29 104 57 451 Fragments arranged by size: 451 133 111 104 82 79 57 42 29 18 13 12 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 100 132 1140 Size: 100 32 1008 Fragments arranged by size: 1008 100 32 KasI G'GCGC_C Cuts at: 0 456 628 685 1140 Size: 456 172 57 455 Fragments arranged by size: 456 455 172 57 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1127 1140 Size: 1127 13 MslI CAynn'nnrTG Cuts at: 0 937 952 1051 1140 Size: 937 15 99 89 Fragments arranged by size: 937 99 89 15 MspA1I CmG'CkG Cuts at: 0 114 278 333 600 818 857 905 1140 Size: 114 164 55 267 218 39 48 235 Fragments arranged by size: 267 235 218 164 114 55 48 39 NaeI GCC'GGC Cuts at: 0 99 134 1140 Size: 99 35 1006 Fragments arranged by size: 1006 99 35 NarI GG'CG_CC Cuts at: 0 457 629 686 1140 Size: 457 172 57 454 Fragments arranged by size: 457 454 172 57 NgoMIV G'CCGG_C Cuts at: 0 97 132 1140 Size: 97 35 1008 Fragments arranged by size: 1008 97 35 PasI CC'CwG_GG Cuts at: 0 680 1140 Size: 680 460 PmlI CAC'GTG Cuts at: 0 387 989 1140 Size: 387 602 151 Fragments arranged by size: 602 387 151 PpuMI rG'GwC_Cy Cuts at: 0 636 1092 1140 Size: 636 456 48 Fragments arranged by size: 636 456 48 PspOMI G'GGCC_C Cuts at: 0 27 65 1140 Size: 27 38 1075 Fragments arranged by size: 1075 38 27 PstI C_TGCA'G Cuts at: 0 329 398 485 862 1140 Size: 329 69 87 377 278 Fragments arranged by size: 377 329 278 87 69 PvuII CAG'CTG Cuts at: 0 857 1140 Size: 857 283 SacII CC_GC'GG Cuts at: 0 906 1140 Size: 906 234 SanDI GG'GwC_CC Cuts at: 0 636 1140 Size: 636 504 SfcI C'TryA_G Cuts at: 0 325 394 481 858 897 1140 Size: 325 69 87 377 39 243 Fragments arranged by size: 377 325 243 87 69 39 SfoI GGC'GCC Cuts at: 0 458 630 687 1140 Size: 458 172 57 453 Fragments arranged by size: 458 453 172 57 SmaI CCC'GGG Cuts at: 0 63 634 1140 Size: 63 571 506 Fragments arranged by size: 571 506 63 SnaBI TAC'GTA Cuts at: 0 1128 1140 Size: 1128 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 313 1140 Size: 313 827 XmaI C'CCGG_G Cuts at: 0 61 632 1140 Size: 61 571 508 Fragments arranged by size: 571 508 61 XmnI GAAnn'nnTTC Cuts at: 0 526 1140 Size: 526 614 Enzymes that do cut: AcuI AfeI AflIII AlfI AlwNI ApaI AvaI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BmrI BpmI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI BtgI BtsI EaeI EagI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV PasI PmlI PpuMI PspOMI PstI PvuII SacII SanDI SfcI SfoI SmaI SnaBI TaqII XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmtI BplI Bpu10I BpuEI BsaI BsaWI BsiHKAI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI