Restriction map for
pFN21AE5469
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwNI 509 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 220 ATTAAT AT^TAAT
AsnI 220 ATTAAT AT^TAAT
Asp700I 752 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 212 TGATCA T^GATCA
BsaI 115 GAGACC GGTCTC(1/5)
BsmFI 312 GTCCC GGGAC(10/14)
BspHI 215 TCATGA T^CATGA
BsrFI 308 GCCGGT [GA]^CCGG[CT]
BstXI 596 CCAATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 308 GCCGGT [GA]^CCGG[CT]
DsaI 377 CCATGG C^C[GA][CT]GG
EcoRV 415 GATATC GAT^ATC
EcoT14I 377 CCATGG C^C[AT][AT]GG
FbaI 212 TGATCA T^GATCA
HapII 309 CCGG C^CGG
HindIII 328 AAGCTT A^AGCTT
HpaII 309 CCGG C^CGG
MseI 221 TTAA T^TAA
MspI 309 CCGG C^CGG
MwoI 514 GCTGGTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 377 CCATGG C^CATGG
NheI 244 GCTAGC G^CTAGC
NlaIV 311 GGTCCC GG[ACGT]^[ACGT]CC
PflMI 508 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 744 GACTC GAGTC(4/5)
PshBI 220 ATTAAT AT^TAAT
PstI 257 CTGCAG CTGCA^G
RcaI 215 TCATGA T^CATGA
SmlI 827 CTCAAG C^T[CT][GA]AG
SphI 790 GCATGC GCATG^C
StyI 377 CCATGG C^C[AT][AT]GG
Tru9I 221 TTAA T^TAA
Van91I 508 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 752 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 157 GTCTAC GT^[AC][GT]AC
424 GTAGAC
AcsI 100 AAATTC [GA]^AATT[CT]
398 AAATTC
AlwI 882 GATCC GGATC(4/5)
363 GGATC
ApoI 100 AAATTC [GA]^AATT[CT]
398 AAATTC
AvaII 311 GGTCC G^G[AT]CC
381 GGACC
BbvI 26 GCAGC GCAGC(8/12)
694 GCAGC
BpmI 73 CTGGAG CTGGAG(16/14)
716 CTGGAG
BsaJI 66 CCTGGG C^C[ACGT][ACGT]GG
377 CCATGG
BsiYI 66 CCTGGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
508 CCAGATGCTGG
BslI 66 CCTGGGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
508 CCAGATGCTGG
BsmAI 115 GAGAC GTCTC(1/5)
502 GTCTC
BspMI 118 ACCTGC ACCTGC(4/8)
355 GCAGGT
BsrDI 540 CATTGC GCAATG(2/0)
847 CATTGC
BsrI 421 CCAGT ACTGG(1/-1)
549 CCAGT
BstF5I 264 CATCC GGATG(2/0)
153 GGATG
FokI 264 CATCC GGATG(9/13)
153 GGATG
MslI 372 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
873 CATCTCCATG
NspI 387 ACATGC [GA]CATG^[CT]
790 GCATGC
PvuII 695 CAGCTG CAG^CTG
713 CAGCTG
SfaNI 511 GATGC GCATC(5/9)
779 GCATC
SfcI 257 CTGCAG C^T[GA][CT]AG
350 CTATAG
TseI 26 GCAGC G^C[AT]GC
694 GCAGC
TspRI 786 CACTG CA[GC]TG[ACGT][ACGT]^
803 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AluI BfaI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MspA1I MvaI NdeII NlaIII Sau3AI
Sau96I ScrFI TfiI Tsp45I Tsp509I