##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       KasI        
Ksp632I     KspI        MaeI        MamI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        501  GGTACC               G^GTACC
AccI           46  GTCTAC               GT^[AC][GT]AC
AcsI          523  GAATTT               [GA]^AATT[CT]
AflIII         58  ACATGT               A^C[GA][CT]GT
ApoI          523  GAATTT               [GA]^AATT[CT]
Asp718I       501  GGTACC               G^GTACC
AvaI          807  CTCGGG               C^[CT]CG[GA]G
AvaII         216  GGACC                G^G[AT]CC
BanI          501  GGTACC               G^G[CT][GA]CC
BbvCI         270  GCTGAGG              CCTCAGC(-5/-2)
BciVI         679  GTATCC               GTATCC(6/5)
BmyI          765  GTGCCC               G[AGT]GC[ACT]^C
BsaAI          19  TACGTG               [CT]AC^GT[GA]
BsiYI         311  CCAAGAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          311  CCAAGAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI         807  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      765  GTGCCC               G[AGT]GC[ACT]^C
BspLU11I       58  ACATGT               A^CATGT
BsrDI         434  GCAATG               GCAATG(2/0)
BstF5I        358  GGATG                GGATG(2/0)
FokI          358  GGATG                GGATG(9/13)
HincII        601  GTTGAC               GT[CT]^[GA]AC
HindII        601  GTTGAC               GT[CT]^[GA]AC
HphI          708  GGTGA                GGTGA(8/7)
KpnI          501  GGTACC               GGTAC^C
MaeII          20  ACGT                 A^CGT
MslI          430  CATGGCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI          132  CATATG               CA^TATG
NlaIV         501  GGTACC               GG[ACGT]^[ACGT]CC
NspI           58  ACATGT               [GA]CATG^[CT]
PleI          170  GACTC                GAGTC(4/5)
PshAI         170  GACTCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           34  CTGCAG               CTGCA^G
SfaNI         553  GATGC                GCATC(5/9)
SfcI           34  CTGCAG               C^T[GA][CT]AG
TfiI          562  GATTC                G^A[AT]TC
XcmI           41  CCACCGTCTACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AluI          395  AGCT                 AG^CT
              496  AGCT                
BsmFI         263  GGGAC                GGGAC(10/14)
              439  GGGAC               
Cac8I           3  GCAGGC               GC[ACGT]^[ACGT]GC
               10  GCGTGC              
DpnII         427  GATC                 ^GATC
              711  GATC                
HinfI         170  GACTC                G^A[ACGT]TC
              562  GATTC               
MboI          427  GATC                 ^GATC
              711  GATC                
NdeII         427  GATC                 ^GATC
              711  GATC                
Sau3AI        427  GATC                 ^GATC
              711  GATC                
TspRI         389  CAGTG                CA[GC]TG[ACGT][ACGT]^
              786  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AfaI        BbvI        BsaJI       BsrI        BssKI       
BstNI       Cfr13I      DdeI        EcoRII      Fnu4HI      
HaeIII      ItaI        MaeIII      MnlI        MseI        
MvaI        NlaIII      RsaI        Sau96I      ScrFI       
Tru9I       TseI        Tsp45I      Tsp509I