Restriction map for
pFN21AE1180
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 1257 CCGC CCGC(-3/-1)
Alw44I 1456 GTGCAC G^TGCAC
AlwNI 67 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1281 CCTGAGG CC^T[ACGT]AGG
ApaLI 1456 GTGCAC G^TGCAC
AspHI 1456 GTGCAC G[AT]GC[AT]^C
BamHI 75 GGATCC G^GATCC
BbsI 1092 GAAGAC GAAGAC(2/6)
BfaI 130 CTAG C^TAG
BmyI 1456 GTGCAC G[AGT]GC[ACT]^C
BpuAI 1092 GAAGAC GAAGAC(2/6)
BsaWI 159 ACCGGA [AT]^CCGG[AT]
BseRI 523 CTCCTC GAGGAG(10/8)
BsiHKAI 1456 GTGCAC G[AT]GC[AT]^C
Bsp1286I 1456 GTGCAC G[AGT]GC[ACT]^C
Bsu36I 1281 CCTGAGG CC^T[ACGT]AGG
DraII 1285 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 196 CCATGG C^C[GA][CT]GG
Eco81I 1281 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1285 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 196 CCATGG C^C[AT][AT]GG
HaeIII 17 GGCC GG^CC
HapII 160 CCGG C^CGG
HpaII 160 CCGG C^CGG
HphI 50 TCACC GGTGA(8/7)
MaeI 130 CTAG C^TAG
MspA1I 70 CAGCTG C[AC]G^C[GT]G
MspI 160 CCGG C^CGG
MwoI 1250 GCTTCTGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 196 CCATGG C^CATGG
NdeI 787 CATATG CA^TATG
PpuMI 1285 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 70 CAGCTG CAG^CTG
SapI 190 GAAGAGC GCTCTTC(1/4)
SfcI 120 CTACAG C^T[GA][CT]AG
SmlI 538 CTTGAG C^T[CT][GA]AG
StyI 196 CCATGG C^C[AT][AT]GG
TfiI 272 GAATC G^A[AT]TC
Tsp45I 419 GTGAC ^GT[GC]AC
XcmI 235 CCAGATGAAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1263 GTCTAC GT^[AC][GT]AC
1610 GTAGAC
AvaII 762 GGACC G^G[AT]CC
1286 GGACC
BbvI 66 GCAGC GCAGC(8/12)
69 GCAGC
BpmI 751 CTCCAG CTGGAG(16/14)
112 CTGGAG
BsaI 319 GAGACC GGTCTC(1/5)
1134 GAGACC
BspHI 1149 TCATGA T^CATGA
1439 TCATGA
BsrI 19 CCAGT ACTGG(1/-1)
1509 CCAGT
BstYI 75 GGATCC [GA]^GATC[CT]
733 GGATCT
HindIII 535 AAGCTT A^AGCTT
563 AAGCTT
MaeII 35 ACGT A^CGT
442 ACGT
MaeIII 419 GTGAC ^GT[ACGT]AC
1011 GTTAC
MflI 75 GGATCC [GA]^GATC[CT]
733 GGATCT
PleI 421 GACTC GAGTC(4/5)
867 GACTC
RcaI 1149 TCATGA T^CATGA
1439 TCATGA
TaqI 1779 TCGA T^CGA
1872 TCGA
TseI 66 GCAGC G^C[AT]GC
69 GCAGC
TthHB8I 1779 TCGA T^CGA
1872 TCGA
XhoII 75 GGATCC [GA]^GATC[CT]
733 GGATCT
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BsaJI BsiYI BslI BsmAI
BsmI Bsp1407I BsrGI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRI EcoRII Fnu4HI FokI HinfI
ItaI Ksp632I MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SspBI Tru9I Tsp509I TspRI
XmnI