Restriction map for
pFN21AE1180
(Circular) (Six-base) MAPSORT of: pFN21AE1180.seq Check: 9916 from: 1 to: 6796
pFN21AE1180 6796 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4606 278
Size: 53 83 186 936 3070 2468
Fragments arranged by size:
3070 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 3616 4139 1937
Size: 1332 347 523 4594
Fragments arranged by size:
4594 1332 523 347
Acc65I G'GTAC_C
Cuts at: 4117 4117
Size: 6796
AclI AA'CG_TT
Cuts at: 1563 4923 5296 1563
Size: 3360 373 3063
Fragments arranged by size:
3360 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2213 4859 6243 6511 1632
Size: 581 2646 1384 268 1917
Fragments arranged by size:
2646 1917 1384 581 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6599
AflIII A'CryG_T
Cuts at: 1203 5695 6672 1203
Size: 4492 977 1327
Fragments arranged by size:
4492 1327 977
AgeI A'CCGG_T
Cuts at: 5627 5627
Size: 6796
AhdI GACnn_n'nnGTC
Cuts at: 5525 5525
Size: 6796
AleI CACnn'nnGTG
Cuts at: 1166 3216 3218 1166
Size: 2050 2 4744
Fragments arranged by size:
4744 2050 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6796
AlwNI CAG_nnn'CTG
Cuts at: 2077 4539 6111 2077
Size: 2462 1572 2762
Fragments arranged by size:
2762 2462 1572
ApaLI G'TGCA_C
Cuts at: 3461 4853 6009 3461
Size: 1392 1156 4248
Fragments arranged by size:
4248 1392 1156
ApoI r'AATT_y
Cuts at: 1493 2219 2576 2631 2852 3207 3335 3433 3671
Size: 726 357 55 221 355 128 98 238
Cuts at: 3671 3711 3769 3879 4010 4047 4105 4331 4483
Size: 40 58 110 131 37 58 226 152
Cuts at: 4483 6592 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 726 357 355 238 226 221
152 131 128 110 98 58 58 55
40 37
AseI AT'TA_AT
Cuts at: 160 1032 5350 160
Size: 872 4318 1606
Fragments arranged by size:
4318 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6796
AvaI C'yCGr_G
Cuts at: 1870 1943 4121 1870
Size: 73 2178 4545
Fragments arranged by size:
4545 2178 73
BamHI G'GATC_C
Cuts at: 2080 4126 4493 2080
Size: 2046 367 4383
Fragments arranged by size:
4383 2046 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4117 5572 618
Size: 325 217 627 2330 1455 1842
Fragments arranged by size:
2330 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4115 1391
Size: 2724 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3104 928
Size: 971 1205 4620
Fragments arranged by size:
4620 1205 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4987 5021 1903
Size: 34 3050 34 3678
Fragments arranged by size:
3678 3050 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4688 5898 4688
Size: 1210 5586
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6796
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5407 136
Size: 107 122 71 631 605 3735 1525
Fragments arranged by size:
3735 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6791 6791
Size: 6796
BlpI GC'TnA_GC
Cuts at: 4213 4213
Size: 6796
Bme1580I G_kGCm'C
Cuts at: 1790 3465 4857 6013 1790
Size: 1675 1392 1156 2573
Fragments arranged by size:
2573 1675 1392 1156
BmgBI CAC'GTC
Cuts at: 1318 2041 1318
Size: 723 6073
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3508 4527 5485
Size: 416 53 83 399 24 2091 1019 958
Cuts at: 5485 442
Size: 1753
Fragments arranged by size:
2091 1753 1019 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6796
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2741 5456 1239
Size: 899 603 2715 2579
Fragments arranged by size:
2715 2579 899 603
Bpu10I CC'TnA_GC
Cuts at: 2085 2085
Size: 6796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 4921 5786 6084 2564
Size: 2357 865 298 3276
Fragments arranged by size:
3276 2357 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2318 3133 5459 882
Size: 617 819 815 2326 2219
Fragments arranged by size:
2326 2219 819 815 617
BsaAI yAC'GTr
Cuts at: 493 1204 4587 493
Size: 711 3383 2702
Fragments arranged by size:
3383 2702 711
BsaBI GATnn'nnATC
Cuts at: 6790 6790
Size: 6796
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4603 4985 5620 275
Size: 53 83 186 936 3070 382 635 1451
Fragments arranged by size:
3070 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2164 5229 5627 5901 6048 6459 2164
Size: 3065 398 274 147 411 2501
Fragments arranged by size:
3065 2501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6571
Fragments arranged by size:
6571 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2519 1186
Size: 1333 5463
BseYI C'CCAG_C
Cuts at: 5999 6495 5999
Size: 496 6300
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6223
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4246 5007 6035 1540
Size: 275 186 2245 761 1028 2301
Fragments arranged by size:
2301 2245 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3465 4857 4942 6013 3465
Size: 1392 85 1071 4248
Fragments arranged by size:
4248 1392 1071 85
BsmI GAATG_Cn'
Cuts at: 2672 2819 3659 4028 4307 4400 2672
Size: 147 840 369 279 93 5068
Fragments arranged by size:
5068 840 369 279 147 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3465 4115 4857 4942 6013 1391
Size: 399 1675 650 742 85 1071 2174
Fragments arranged by size:
2174 1675 1071 742 650 399 85
BspEI T'CCGG_A
Cuts at: 6459 6459
Size: 6796
BspHI T'CATG_A
Cuts at: 3154 3444 4685 6415 3154
Size: 290 1241 1730 3535
Fragments arranged by size:
3535 1730 1241 290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4151 844
Size: 3307 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4683 1394
Size: 429 2860 3507
Fragments arranged by size:
3507 2860 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5291 5465 65
Size: 1183 4043 174 1396
Fragments arranged by size:
4043 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5440 5627 1700
Size: 110 3630 187 2869
Fragments arranged by size:
3630 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2776 3364 3952 96
Size: 2680 588 588 2940
Fragments arranged by size:
2940 2680 588 588
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6796
BssSI C'ACGA_G
Cuts at: 4856 5868 4856
Size: 1012 5784
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6796
BstBI TT'CG_AA
Cuts at: 5600 5600
Size: 6796
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6796
BstYI r'GATC_y
Cuts at: 1972 2080 2738 4126 4493 4878 4895 6347 6433
Size: 108 658 1388 367 385 17 1452 86
Cuts at: 6433 6445 6791 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1388 658 385 367 346 108
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3287 3287
Size: 6796
BtgI C'CryG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 5108
Fragments arranged by size:
5108 1136 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6057
Fragments arranged by size:
6057 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 4312 5106 5126 2824
Size: 1488 794 20 4494
Fragments arranged by size:
4494 1488 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6796
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6761
DraI TTT'AAA
Cuts at: 4100 4100
Size: 6796
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5803 809
Size: 507 4487 1802
Fragments arranged by size:
4487 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4243 5132 6761
Size: 54 1425 50 275 18 2413 889 1629
Cuts at: 6761 8
Size: 43
Fragments arranged by size:
2413 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4243 1537
Size: 275 2431 4090
Fragments arranged by size:
4090 2431 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2280 2331 4726 1353
Size: 837 90 51 2395 3423
Fragments arranged by size:
3423 2395 837 90 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5383 5757 5903 387
Size: 1064 138 3794 374 146 1280
Fragments arranged by size:
3794 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2213 2741 4859 5456 6243 6511
Size: 393 506 75 528 2118 597 787 268
Cuts at: 6511 1239
Size: 1524
Fragments arranged by size:
2118 1524 787 597 528 506 393 268
75
EcoO109I rG'GnC_Cy
Cuts at: 3291 3291
Size: 6796
EcoRI G'AATT_C
Cuts at: 2219 3433 3711 3769 3879 4047 4105 4483 2219
Size: 1214 278 58 110 168 58 378 4532
Fragments arranged by size:
4532 1214 378 278 168 110 58 58
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2636 2636
Size: 6796
FspI TGC'GCA
Cuts at: 5302 5302
Size: 6796
HaeII r_GCGC'y
Cuts at: 1678 5943 1678
Size: 4265 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3844 3876 5437 5469 5511
Size: 32 135 32 2272 32 1561 32 42
Cuts at: 5511 5543 1373
Size: 32 2626
Fragments arranged by size:
2626 2272 1561 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4140 4386 669
Size: 988 281 2202 246 3079
Fragments arranged by size:
3079 2202 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2540 2568 748
Size: 1792 28 4976
Fragments arranged by size:
4976 1792 28
HpaI GTT'AAC
Cuts at: 4386 4386
Size: 6796
KpnI G_GTAC'C
Cuts at: 4121 4121
Size: 6796
MfeI C'AATT_G
Cuts at: 4395 4395
Size: 6796
MluI A'CGCG_T
Cuts at: 6672 6672
Size: 6796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2163 2745 5910 6094 1305
Size: 858 582 3165 184 2007
Fragments arranged by size:
3165 2007 858 582 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4974
Fragments arranged by size:
4974 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3159 3216 3218 4754 5113 5272 518
Size: 648 1993 57 2 1536 359 159 2042
Fragments arranged by size:
2042 1993 1536 648 359 159 57 2
MspA1I CmG'CkG
Cuts at: 1606 2077 4212 4889 6037 6282 1606
Size: 471 2135 677 1148 245 2120
Fragments arranged by size:
2135 2120 1148 677 471 245
NcoI C'CATG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 5108
Fragments arranged by size:
5108 1136 552
NdeI CA'TA_TG
Cuts at: 387 2793 387
Size: 2406 4390
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6796
NotI GC'GGCC_GC
Cuts at: 4243 4243
Size: 6796
NspI r_CATG'y
Cuts at: 1132 1229 4154 5699 1132
Size: 97 2925 1545 2229
Fragments arranged by size:
2925 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2017 1348
Size: 669 6127
PciI A'CATG_T
Cuts at: 5695 5695
Size: 6796
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6796
PfoI T'CCnGG_A
Cuts at: 2280 2557 2280
Size: 277 6519
PmeI GTTT'AAAC
Cuts at: 4100 4100
Size: 6796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2334 2366 4865 4897 6378 6410 2334
Size: 32 2499 32 1481 32 2720
Fragments arranged by size:
2720 2499 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3291 3291
Size: 6796
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6796
PsiI TTA'TAA
Cuts at: 4366 4366
Size: 6796
PstI C_TGCA'G
Cuts at: 830 4148 830
Size: 3318 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6796
PvuII CAG'CTG
Cuts at: 2077 2077
Size: 6796
SalI G'TCGA_C
Cuts at: 1936 4138 1936
Size: 2202 4594
SapI GCTCTTCn'nnn_
Cuts at: 2190 2190
Size: 6796
SbfI CC_TGCA'GG
Cuts at: 4148 4148
Size: 6796
ScaI AGT'ACT
Cuts at: 5044 6756 5044
Size: 1712 5084
SfcI C'TryA_G
Cuts at: 826 1045 2125 4144 5279 5960 6151 826
Size: 219 1080 2019 1135 681 191 1471
Fragments arranged by size:
2019 1471 1135 1080 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6796
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6796
SmaI CCC'GGG
Cuts at: 1872 4123 1872
Size: 2251 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2543 4900 5801 6063 820
Size: 197 926 600 2357 901 262 1553
Fragments arranged by size:
2357 1553 926 901 600 262 197
SnaBI TAC'GTA
Cuts at: 493 4587 493
Size: 4094 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6796
SphI G_CATG'C
Cuts at: 1132 4154 1132
Size: 3022 3774
SspI AAT'ATT
Cuts at: 5 52 4720 5
Size: 47 4668 2081
Fragments arranged by size:
4668 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6796
StyI C'CwwG_G
Cuts at: 513 1065 2201 4235 513
Size: 552 1136 2034 3074
Fragments arranged by size:
3074 2034 1136 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 4823 4840 4993 5178 5623 325
Size: 1255 492 2751 17 153 185 445 1498
Fragments arranged by size:
2751 1498 1255 492 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2776 3364 3952
Size: 274 80 33 51 1443 799 588 588
Cuts at: 3952 3992 5042 6754 96
Size: 40 1050 1712 138
Fragments arranged by size:
1712 1443 1050 799 588 588 274 138
80 51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6756 6788 6756
Size: 32 6764
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6796
XbaI T'CTAG_A
Cuts at: 4132 4132
Size: 6796
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2247 2247
Size: 6796
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6796
XmaI C'CCGG_G
Cuts at: 1870 4121 1870
Size: 2251 4545
XmnI GAAnn'nnTTC
Cuts at: 2678 2834 2918 3127 3170 3254 3379 3547 3590
Size: 156 84 209 43 84 125 168 43
Cuts at: 3590 3715 3758 3842 3883 3967 4010 4925 2678
Size: 125 43 84 41 84 43 915 4549
Fragments arranged by size:
4549 915 209 168 156 125 125 84
84 84 84 43 43 43 43 41
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4604 276
Size: 53 83 186 936 3070 2468
Fragments arranged by size:
3070 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI