################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AlwNI AocI Aor51HI AscI Asp718I AspI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BspMI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FauI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 739 TCCGGA T^CCGGA AhdI 781 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1510 GTGCAC G^TGCAC ApaI 888 GGGCCC GGGCC^C ApaLI 1510 GTGCAC G^TGCAC AspEI 781 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 1679 CTCGAG C^[CT]CG[GA]G BbsI 1284 GTCTTC GAAGAC(2/6) BcgI 675 GCAGTACATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1703 TGATCA T^GATCA BglII 219 AGATCT A^GATCT BpuAI 1284 GTCTTC GAAGAC(2/6) BsaBI 1684 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 739 TCCGGA T^CCGGA BsgI 1049 GTGCAG GTGCAG(16/14) BsmFI 264 GGGAC GGGAC(10/14) BsoBI 1679 CTCGAG C^[CT]CG[GA]G Bsp1407I 1240 TGTACA T^GTACA BspEI 739 TCCGGA T^CCGGA BsrBI 4 GAGCGG CCGCTC(-3/-3) BsrGI 1240 TGTACA T^GTACA BstEII 1783 GGTGACC G^GT[ACGT]ACC BstF5I 627 CATCC GGATG(2/0) BstPI 1783 GGTGACC G^GT[ACGT]ACC DraI 451 TTTAAA TTT^AAA DrdI 1153 GACTGTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 781 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1783 GGTGACC G^GT[ACGT]ACC EcoT22I 1614 ATGCAT ATGCA^T FbaI 1703 TGATCA T^GATCA FokI 627 CATCC GGATG(9/13) HaeII 827 AGCGCC [GA]GCGC^[CT] MaeII 1032 ACGT A^CGT MamI 1684 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 739 TCCGGA T^CCGGA MslI 1269 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1271 CCATGG C^CATGG NsiI 1614 ATGCAT ATGCA^T NspI 1612 GCATGC [GA]CATG^[CT] PaeR7I 1679 CTCGAG C^TCGAG SacI 725 GAGCTC GAGCT^C ScaI 754 AGTACT AGT^ACT SfaNI 507 GCATC GCATC(5/9) SphI 1612 GCATGC GCATG^C SspBI 1240 TGTACA T^GTACA SspI 1754 AATATT AAT^ATT TaqI 1680 TCGA T^CGA TthHB8I 1680 TCGA T^CGA XhoI 1679 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AseI 1060 ATTAAT AT^TAAT 1087 ATTAAT AsnI 1060 ATTAAT AT^TAAT 1087 ATTAAT AvaII 41 GGACC G^G[AT]CC 1393 GGACC BalI 1008 TGGCCA TGG^CCA 1631 TGGCCA BanI 1725 GGTGCC G^G[CT][GA]CC 1946 GGCACC BanII 725 GAGCTC G[GA]GC[CT]^C 888 GGGCCC BpmI 22 CTCCAG CTGGAG(16/14) 722 CTGGAG BsaWI 374 TCCGGT [AT]^CCGG[AT] 739 TCCGGA BseRI 728 CTCCTC GAGGAG(10/8) 136 GAGGAG BsmI 854 GAATGC GAATGC(1/-1) 1343 GCATTC BstAPI 507 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1607 GCACGGCATGC CfoI 19 GCGC GCG^C 828 GCGC DraII 888 GGGCCCT [GA]G^G[ACGT]CC[CT] 1817 GGGGCCT DsaI 1271 CCATGG C^C[GA][CT]GG 1418 CCACGG EaeI 1008 TGGCCA [CT]^GGCC[GA] 1631 TGGCCA EcoNI 1657 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1821 CCTTTTTGAGG EcoO109I 888 GGGCCCT [GA]G^G[ACGT]CC[CT] 1817 GGGGCCT HhaI 19 GCGC GCG^C 828 GCGC HinP1I 19 GCGC G^CGC 828 GCGC MluNI 1008 TGGCCA TGG^CCA 1631 TGGCCA MscI 1008 TGGCCA TGG^CCA 1631 TGGCCA MspA1I 776 CAGCTG C[AC]G^C[GT]G 1868 CAGCTG PshBI 1060 ATTAAT AT^TAAT 1087 ATTAAT PstI 695 CTGCAG CTGCA^G 746 CTGCAG PvuII 776 CAGCTG CAG^CTG 1868 CAGCTG Tsp45I 1640 GTGAC ^GT[GC]AC 1784 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII XmnI