Restriction map for
pFN21AE3429
(Circular) (Six-base) MAPSORT of: pFN21AE3429.seq Check: 6750 from: 1 to: 6670
pFN21AE3429 6670 bp
With 182 enzymes: SgfI *
March 28, 2012 12:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 3991 3991
Size: 6670
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 3552 4733 6117 6385 1632
Size: 776 1144 1181 1384 268 1917
Fragments arranged by size:
1917 1384 1181 1144 776 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 5569 6546 1203
Size: 4366 977 1327
Fragments arranged by size:
4366 1327 977
AgeI A'CCGG_T
Cuts at: 5501 5501
Size: 6670
AhdI GACnn_n'nnGTC
Cuts at: 2791 5399 2791
Size: 2608 4062
AleI CACnn'nnGTG
Cuts at: 1166 3278 1166
Size: 2112 4558
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6670
AlwNI CAG_nnn'CTG
Cuts at: 4413 5985 4413
Size: 1572 5098
ApaI G_GGCC'C
Cuts at: 2897 2897
Size: 6670
ApaLI G'TGCA_C
Cuts at: 3515 4727 5883 3515
Size: 1212 1156 4302
Fragments arranged by size:
4302 1212 1156
ApoI r'AATT_y
Cuts at: 1493 2315 3172 3433 3770 3979 4205 4357 6466
Size: 822 857 261 337 209 226 152 2109
Cuts at: 6466 1493
Size: 1697
Fragments arranged by size:
2109 1697 857 822 337 261 226 209
152
AseI AT'TA_AT
Cuts at: 160 1032 3066 3093 5224 160
Size: 872 2034 27 2131 1606
Fragments arranged by size:
2131 2034 1606 872 27
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 3684 3995 1870
Size: 73 1741 311 4545
Fragments arranged by size:
4545 1741 311 73
BamHI G'GATC_C
Cuts at: 4000 4367 4000
Size: 367 6303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3730 3951 3991 5446 618
Size: 325 217 627 1943 221 40 1455 1842
Fragments arranged by size:
1943 1842 1455 627 325 221 217 40
BanII G_rGCy'C
Cuts at: 1391 2734 2897 3989 1391
Size: 1343 163 1092 4072
Fragments arranged by size:
4072 1343 1092 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3282 928
Size: 971 1383 4316
Fragments arranged by size:
4316 1383 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2669 2703 4861 4895 1903
Size: 34 732 34 2158 34 3678
Fragments arranged by size:
3678 2158 732 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4562 5772 4562
Size: 1210 5460
BclI T'GATC_A
Cuts at: 1548 3708 1548
Size: 2160 4510
BfrBI ATG'CAT
Cuts at: 3621 3621
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5281 136
Size: 107 122 71 631 605 3609 1525
Fragments arranged by size:
3609 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2224 6665 2224
Size: 4441 2229
BlpI GC'TnA_GC
Cuts at: 4087 4087
Size: 6670
Bme1580I G_kGCm'C
Cuts at: 1790 2897 3519 4731 5887 1790
Size: 1107 622 1212 1156 2573
Fragments arranged by size:
2573 1212 1156 1107 622
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6670
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4401 5359 442
Size: 416 53 83 399 24 2984 958 1753
Fragments arranged by size:
2984 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2012 2748 5330 1239
Size: 773 736 2582 2579
Fragments arranged by size:
2582 2579 773 736
Bpu10I CC'TnA_GC
Cuts at: 3197 3197
Size: 6670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 3213 3461 3473 4795 5660 5958 2507
Size: 706 248 12 1322 865 298 3219
Fragments arranged by size:
3219 1322 865 706 298 248 12
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5333 882
Size: 617 3834 2219
Fragments arranged by size:
3834 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4461 493
Size: 711 3257 2702
Fragments arranged by size:
3257 2702 711
BsaBI GATnn'nnATC
Cuts at: 3693 6664 3693
Size: 2971 3699
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4477 4859 5494 275
Size: 53 83 186 936 2944 382 635 1451
Fragments arranged by size:
2944 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2379 2744 5103 5501 5775 5922 6333 2379
Size: 365 2359 398 274 147 411 2716
Fragments arranged by size:
2716 2359 411 398 365 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2873 2903 1178
Size: 30 165 30 1470 30 4945
Fragments arranged by size:
4945 1470 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2724 1186
Size: 970 568 5132
Fragments arranged by size:
5132 970 568
BseYI C'CCAG_C
Cuts at: 2265 2769 5873 6369 2265
Size: 504 3104 496 2566
Fragments arranged by size:
3104 2566 504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3075 1163
Size: 573 1339 4758
Fragments arranged by size:
4758 1339 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4120 4881 5909 1540
Size: 275 186 2119 761 1028 2301
Fragments arranged by size:
2301 2119 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2734 3519 3614 4731 4816 5887 2734
Size: 785 95 1117 85 1071 3517
Fragments arranged by size:
3517 1117 1071 785 95 85
BsmI GAATG_Cn'
Cuts at: 2865 3348 4181 4274 2865
Size: 483 833 93 5261
Fragments arranged by size:
5261 833 483 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2734 2897 3519 3614 3989 4731 4816
Size: 399 944 163 622 95 375 742 85
Cuts at: 4816 5887 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 944 742 622 399 375 163
95 85
BspEI T'CCGG_A
Cuts at: 2744 6333 2744
Size: 3589 3081
BspHI T'CATG_A
Cuts at: 4559 6289 4559
Size: 1730 4940
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4025 844
Size: 3181 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 4557 1394
Size: 429 188 2546 3507
Fragments arranged by size:
3507 2546 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 2331 3079 3699 5165 5339 65
Size: 1183 1083 748 620 1466 174 1396
Fragments arranged by size:
1466 1396 1183 1083 748 620 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5314 5501 1700
Size: 110 3504 187 2869
Fragments arranged by size:
3504 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3245 96
Size: 3149 3521
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6670
BssSI C'ACGA_G
Cuts at: 4730 5742 4730
Size: 1012 5658
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2518 3618 794
Size: 1724 1100 3846
Fragments arranged by size:
3846 1724 1100
BstBI TT'CG_AA
Cuts at: 5474 5474
Size: 6670
BstEII G'GTnAC_C
Cuts at: 1184 3788 1184
Size: 2604 4066
BstYI r'GATC_y
Cuts at: 1972 2153 2176 2224 2747 3139 3578 4000 4367
Size: 181 23 48 523 392 439 422 367
Cuts at: 4367 4752 4769 6221 6307 6319 6665 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 523 439 422 392 385 367
346 181 86 48 23 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3276 3423 513
Size: 552 939 1272 147 3760
Fragments arranged by size:
3760 1272 939 552 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5931
Fragments arranged by size:
5931 683 56
BtsI GCAGTG_nn'
Cuts at: 2558 3930 4186 4980 5000 2558
Size: 1372 256 794 20 4228
Fragments arranged by size:
4228 1372 794 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 2458 3974 2458
Size: 1516 5154
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3164 5677 809
Size: 507 1848 2513 1802
Fragments arranged by size:
2513 1848 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3013 3636 4117
Size: 54 1425 50 275 18 1183 623 481
Cuts at: 4117 5006 6635 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1183 889 623 481 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4117 1537
Size: 275 2305 4090
Fragments arranged by size:
4090 2305 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2991 3669 3861 3917 4600 1353
Size: 780 858 678 192 56 683 3423
Fragments arranged by size:
3423 858 780 683 678 192 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2727 5257 5631 5777 387
Size: 1064 138 1138 2530 374 146 1280
Fragments arranged by size:
2530 1280 1138 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2732 2732
Size: 6670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2408 2748 3552 4733 5330 6117
Size: 393 380 396 340 804 1181 597 787
Cuts at: 6117 6385 1239
Size: 268 1524
Fragments arranged by size:
1524 1181 804 787 597 396 393 380
340 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3666 3830 3666
Size: 164 6506
EcoO109I rG'GnC_Cy
Cuts at: 2894 3823 2894
Size: 929 5741
EcoRI G'AATT_C
Cuts at: 3979 4357 3979
Size: 378 6292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3008 3008
Size: 6670
FspI TGC'GCA
Cuts at: 5176 5176
Size: 6670
HaeII r_GCGC'y
Cuts at: 1678 2836 5817 1678
Size: 1158 2981 2531
Fragments arranged by size:
2981 2531 1158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2739 2771 2777 2809 2873
Size: 32 135 32 1167 32 6 32 64
Cuts at: 2873 2905 5311 5343 5385 5417 1373
Size: 32 2406 32 42 32 2626
Fragments arranged by size:
2626 2406 1167 135 64 42 32 32
32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 4014 4260 669
Size: 988 281 2076 246 3079
Fragments arranged by size:
3079 2076 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6670
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KpnI G_GTAC'C
Cuts at: 3995 3995
Size: 6670
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3885 5784 5968 1305
Size: 2580 1899 184 2007
Fragments arranged by size:
2580 2007 1899 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3015 3638 10
Size: 54 1425 343 1183 623 3042
Fragments arranged by size:
3042 1425 1183 623 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3278 4628 4987 5146 518
Size: 648 2112 1350 359 159 2042
Fragments arranged by size:
2112 2042 1350 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2783 3875 4086 4763 5911 6156 1606
Size: 1177 1092 211 677 1148 245 2120
Fragments arranged by size:
2120 1177 1148 1092 677 245 211
NcoI C'CATG_G
Cuts at: 513 1065 2004 3276 513
Size: 552 939 1272 3907
Fragments arranged by size:
3907 1272 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NsiI A_TGCA'T
Cuts at: 3623 3623
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 3621 4028 5573 1132
Size: 97 2392 407 1545 2229
Fragments arranged by size:
2392 2229 1545 407 97
PasI CC'CwG_GG
Cuts at: 1348 2897 1348
Size: 1549 5121
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6670
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 6252 6284 4739
Size: 32 1481 32 5125
Fragments arranged by size:
5125 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PspOMI G'GGCC_C
Cuts at: 2893 2893
Size: 6670
PstI C_TGCA'G
Cuts at: 830 2704 2755 4022 830
Size: 1874 51 1267 3478
Fragments arranged by size:
3478 1874 1267 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
PvuII CAG'CTG
Cuts at: 2783 3875 2783
Size: 1092 5578
SacI G_AGCT'C
Cuts at: 2734 2734
Size: 6670
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SbfI CC_TGCA'GG
Cuts at: 4022 4022
Size: 6670
ScaI AGT'ACT
Cuts at: 2761 4918 6630 2761
Size: 2157 1712 2801
Fragments arranged by size:
2801 2157 1712
SfcI C'TryA_G
Cuts at: 826 1045 2700 2751 3300 4018 5153 5834 6025
Size: 219 1655 51 549 718 1135 681 191
Cuts at: 6025 826
Size: 1471
Fragments arranged by size:
1655 1471 1135 718 681 549 219 191
51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 3997 1872
Size: 2125 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2522 3228 3452 3476 3684 4774
Size: 197 926 579 706 224 24 208 1090
Cuts at: 4774 5675 5937 820
Size: 901 262 1553
Fragments arranged by size:
1553 1090 926 901 706 579 262 224
208 197 24
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 3621 4028 1132
Size: 2489 407 3774
Fragments arranged by size:
3774 2489 407
SspI AAT'ATT
Cuts at: 5 52 3761 4594 5
Size: 47 3709 833 2081
Fragments arranged by size:
3709 2081 833 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6670
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 3276 3496 3561 3765 3818
Size: 552 939 45 1227 220 65 204 53
Cuts at: 3818 3956 4109 513
Size: 138 153 3074
Fragments arranged by size:
3074 1227 939 552 220 204 153 138
65 53 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3502 4697 4714 4867 5052 5497 325
Size: 1255 1922 1195 17 153 185 445 1498
Fragments arranged by size:
1922 1498 1255 1195 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 2759 3245
Size: 274 80 33 51 1443 705 77 486
Cuts at: 3245 3678 4916 6628 96
Size: 433 1238 1712 138
Fragments arranged by size:
1712 1443 1238 705 486 433 274 138
80 77 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6630 6662 6630
Size: 32 6638
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6670
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XhoI C'TCGA_G
Cuts at: 1943 3684 1943
Size: 1741 4929
XmaI C'CCGG_G
Cuts at: 1870 3995 1870
Size: 2125 4545
XmnI GAAnn'nnTTC
Cuts at: 3348 3537 3760 4799 3348
Size: 189 223 1039 5219
Fragments arranged by size:
5219 1039 223 189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI