(Circular) (Six-base) MAPSORT of: pFN21AE6590.seq Check: 793 from: 1 to: 5248 pFN21AE6590 5248 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3058 278 Size: 53 83 186 936 1522 2468 Fragments arranged by size: 2468 1522 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2591 1937 Size: 654 4594 Acc65I G'GTAC_C Cuts at: 2569 2569 Size: 5248 AclI AA'CG_TT Cuts at: 1563 3375 3748 1563 Size: 1812 373 3063 Fragments arranged by size: 3063 1812 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 3311 4695 4963 1632 Size: 470 1209 1384 268 1917 Fragments arranged by size: 1917 1384 1209 470 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5051 AflIII A'CryG_T Cuts at: 1203 4147 5124 1203 Size: 2944 977 1327 Fragments arranged by size: 2944 1327 977 AgeI A'CCGG_T Cuts at: 2337 4079 2337 Size: 1742 3506 AhdI GACnn_n'nnGTC Cuts at: 3977 3977 Size: 5248 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5248 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5248 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2315 Size: 32 5216 AlwNI CAG_nnn'CTG Cuts at: 2291 2491 2991 4563 2291 Size: 200 500 1572 2976 Fragments arranged by size: 2976 1572 500 200 ApaLI G'TGCA_C Cuts at: 3305 4461 3305 Size: 1156 4092 ApoI r'AATT_y Cuts at: 1493 2557 2783 2935 5044 1493 Size: 1064 226 152 2109 1697 Fragments arranged by size: 2109 1697 1064 226 152 AseI AT'TA_AT Cuts at: 160 1032 3802 160 Size: 872 2770 1606 Fragments arranged by size: 2770 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5248 AvaI C'yCGr_G Cuts at: 1870 1943 2573 1870 Size: 73 630 4545 Fragments arranged by size: 4545 630 73 BamHI G'GATC_C Cuts at: 2578 2945 2578 Size: 367 4881 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2468 2569 4024 618 Size: 325 217 627 681 101 1455 1842 Fragments arranged by size: 1842 1455 681 627 325 217 101 BanII G_rGCy'C Cuts at: 1391 2567 1391 Size: 1176 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4277 BbvCI CC'TCA_GC Cuts at: 2032 2406 2459 2032 Size: 374 53 4821 Fragments arranged by size: 4821 374 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3439 3473 1903 Size: 34 1502 34 3678 Fragments arranged by size: 3678 1502 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3140 4350 3140 Size: 1210 4038 BclI T'GATC_A Cuts at: 1548 1548 Size: 5248 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3859 136 Size: 107 122 71 631 605 2187 1525 Fragments arranged by size: 2187 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5243 5243 Size: 5248 BlpI GC'TnA_GC Cuts at: 2665 2665 Size: 5248 Bme1580I G_kGCm'C Cuts at: 1790 2161 2473 3309 4465 1790 Size: 371 312 836 1156 2573 Fragments arranged by size: 2573 1156 836 371 312 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5248 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2979 3937 442 Size: 416 53 83 399 24 1562 958 1753 Fragments arranged by size: 1753 1562 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5248 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2047 2077 2047 Size: 30 5218 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2507 3908 1239 Size: 1088 180 1401 2579 Fragments arranged by size: 2579 1401 1088 180 Bpu10I CC'TnA_GC Cuts at: 2032 2406 2459 2032 Size: 374 53 4821 Fragments arranged by size: 4821 374 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3373 4238 4536 3373 Size: 865 298 4085 Fragments arranged by size: 4085 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3911 882 Size: 617 2412 2219 Fragments arranged by size: 2412 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3039 493 Size: 711 1835 2702 Fragments arranged by size: 2702 1835 711 BsaBI GATnn'nnATC Cuts at: 5242 5242 Size: 5248 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3055 3437 4072 275 Size: 53 83 186 936 1522 382 635 1451 Fragments arranged by size: 1522 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2337 3681 4079 4353 4500 4911 2337 Size: 1344 398 274 147 411 2674 Fragments arranged by size: 2674 1344 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5023 Fragments arranged by size: 5023 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5248 BseYI C'CCAG_C Cuts at: 4451 4947 4451 Size: 496 4752 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2349 2504 1163 Size: 573 613 155 3907 Fragments arranged by size: 3907 613 573 155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2698 3459 4487 1540 Size: 275 186 697 761 1028 2301 Fragments arranged by size: 2301 1028 761 697 275 186 BsiHKAI G_wGCw'C Cuts at: 3309 3394 4465 3309 Size: 85 1071 4092 Fragments arranged by size: 4092 1071 85 BsmI GAATG_Cn' Cuts at: 2759 2852 2759 Size: 93 5155 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2473 2567 3309 3394 4465 1391 Size: 399 371 312 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 371 312 94 85 BspEI T'CCGG_A Cuts at: 4911 4911 Size: 5248 BspHI T'CATG_A Cuts at: 3137 4867 3137 Size: 1730 3518 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2283 2603 844 Size: 1439 320 3489 Fragments arranged by size: 3489 1439 320 BsrBI CCG'CTC Cuts at: 1394 1823 2533 3135 1394 Size: 429 710 602 3507 Fragments arranged by size: 3507 710 602 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2140 3743 3917 65 Size: 1183 892 1603 174 1396 Fragments arranged by size: 1603 1396 1183 892 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2337 3892 4079 1700 Size: 110 527 1555 187 2869 Fragments arranged by size: 2869 1555 527 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5248 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5248 BssSI C'ACGA_G Cuts at: 3308 4320 3308 Size: 1012 4236 BstAPI GCAn_nnn'nTGC Cuts at: 794 2491 794 Size: 1697 3551 BstBI TT'CG_AA Cuts at: 2164 4052 2164 Size: 1888 3360 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5248 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2305 Size: 5248 BstYI r'GATC_y Cuts at: 1972 2578 2945 3330 3347 4799 4885 4897 5243 Size: 606 367 385 17 1452 86 12 346 Cuts at: 5243 1972 Size: 1977 Fragments arranged by size: 1977 1452 606 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3757 Fragments arranged by size: 3757 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4509 Fragments arranged by size: 4509 683 56 BtsI GCAGTG_nn' Cuts at: 2764 3558 3578 2764 Size: 794 20 4434 Fragments arranged by size: 4434 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5248 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5213 DraI TTT'AAA Cuts at: 2552 2552 Size: 5248 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4255 809 Size: 507 2939 1802 Fragments arranged by size: 2939 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2695 3584 5213 Size: 54 1425 50 275 18 865 889 1629 Cuts at: 5213 8 Size: 43 Fragments arranged by size: 1629 1425 889 865 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2695 1537 Size: 275 883 4090 Fragments arranged by size: 4090 883 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2248 3178 1353 Size: 895 930 3423 Fragments arranged by size: 3423 930 895 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3835 4209 4355 387 Size: 1064 138 2246 374 146 1280 Fragments arranged by size: 2246 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2327 2507 3311 3908 4695 4963 Size: 393 470 225 180 804 597 787 268 Cuts at: 4963 1239 Size: 1524 Fragments arranged by size: 1524 804 787 597 470 393 268 225 180 EcoNI CCTnn'n_nnAGG Cuts at: 2283 2283 Size: 5248 EcoO109I rG'GnC_Cy Cuts at: 2272 2272 Size: 5248 EcoRI G'AATT_C Cuts at: 2557 2935 2557 Size: 378 4870 FspI TGC'GCA Cuts at: 3754 3754 Size: 5248 HaeII r_GCGC'y Cuts at: 1678 4395 1678 Size: 2717 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2493 2525 3889 3921 3963 Size: 32 135 32 921 32 1364 32 42 Cuts at: 3963 3995 1373 Size: 32 2626 Fragments arranged by size: 2626 1364 921 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2107 2592 2838 669 Size: 988 281 169 485 246 3079 Fragments arranged by size: 3079 988 485 281 246 169 HindIII A'AGCT_T Cuts at: 748 748 Size: 5248 HpaI GTT'AAC Cuts at: 2107 2838 2107 Size: 731 4517 KpnI G_GTAC'C Cuts at: 2573 2573 Size: 5248 MfeI C'AATT_G Cuts at: 2847 2847 Size: 5248 MluI A'CGCG_T Cuts at: 5124 5124 Size: 5248 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4362 4546 1305 Size: 3057 184 2007 Fragments arranged by size: 3057 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3426 Fragments arranged by size: 3426 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 3206 3565 3724 518 Size: 648 948 1092 359 159 2042 Fragments arranged by size: 2042 1092 948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2405 2664 3341 4489 4734 1606 Size: 799 259 677 1148 245 2120 Fragments arranged by size: 2120 1148 799 677 259 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3757 Fragments arranged by size: 3757 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5248 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5248 NotI GC'GGCC_GC Cuts at: 2695 2695 Size: 5248 NspI r_CATG'y Cuts at: 1132 1229 2606 4151 1132 Size: 97 1377 1545 2229 Fragments arranged by size: 2229 1545 1377 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5248 PciI A'CATG_T Cuts at: 4147 4147 Size: 5248 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5248 PfoI T'CCnGG_A Cuts at: 2093 2511 2093 Size: 418 4830 PmeI GTTT'AAAC Cuts at: 2552 2552 Size: 5248 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3317 3349 4830 4862 3317 Size: 32 1481 32 3703 Fragments arranged by size: 3703 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2272 2272 Size: 5248 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5248 PsiI TTA'TAA Cuts at: 2818 2818 Size: 5248 PstI C_TGCA'G Cuts at: 830 2496 2600 830 Size: 1666 104 3478 Fragments arranged by size: 3478 1666 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5248 SalI G'TCGA_C Cuts at: 1936 2590 1936 Size: 654 4594 SbfI CC_TGCA'GG Cuts at: 2600 2600 Size: 5248 ScaI AGT'ACT Cuts at: 3496 5208 3496 Size: 1712 3536 SfcI C'TryA_G Cuts at: 826 1045 2492 2596 3731 4412 4603 826 Size: 219 1447 104 1135 681 191 1471 Fragments arranged by size: 1471 1447 1135 681 219 191 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5248 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5248 SmaI CCC'GGG Cuts at: 1872 2575 1872 Size: 703 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3352 4253 4515 820 Size: 197 926 1409 901 262 1553 Fragments arranged by size: 1553 1409 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3039 493 Size: 2546 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5248 SphI G_CATG'C Cuts at: 1132 2606 1132 Size: 1474 3774 SspI AAT'ATT Cuts at: 5 52 3172 5 Size: 47 3120 2081 Fragments arranged by size: 3120 2081 47 StuI AGG'CCT Cuts at: 1517 2189 1517 Size: 672 4576 StyI C'CwwG_G Cuts at: 513 1065 2004 2687 513 Size: 552 939 683 3074 Fragments arranged by size: 3074 939 683 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3275 3292 3445 3630 4075 325 Size: 1255 1695 17 153 185 445 1498 Fragments arranged by size: 1695 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3494 5206 96 Size: 274 80 33 51 1443 1517 1712 138 Fragments arranged by size: 1712 1517 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5208 5240 5208 Size: 32 5216 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5248 XbaI T'CTAG_A Cuts at: 2584 2584 Size: 5248 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2302 2449 2302 Size: 147 5101 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5248 XmaI C'CCGG_G Cuts at: 1870 2573 1870 Size: 703 4545 XmnI GAAnn'nnTTC Cuts at: 3377 3377 Size: 5248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3056 276 Size: 53 83 186 936 1522 2468 Fragments arranged by size: 2468 1522 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI