################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaII KasI KpnI KspI MluI MroI MspA1I MspI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 1271 GCGG CCGC(-3/-1) AlwNI 452 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 933 ATTAAT AT^TAAT AsnI 933 ATTAAT AT^TAAT AspHI 500 GAGCTC G[AT]GC[AT]^C AspI 1142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1447 GGTCC G^G[AT]CC BamHI 1440 GGATCC G^GATCC BciVI 397 GTATCC GTATCC(6/5) BclI 569 TGATCA T^GATCA BglII 1546 AGATCT A^GATCT BpmI 1184 CTCCAG CTGGAG(16/14) BsaBI 1696 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 111 GAGACC GGTCTC(1/5) BseRI 1100 GAGGAG GAGGAG(10/8) BsiHKAI 500 GAGCTC G[AT]GC[AT]^C BsmBI 1386 GAGACG CGTCTC(1/5) BsmFI 461 GGGAC GGGAC(10/14) Cac8I 1430 GCAGGC GC[ACGT]^[ACGT]GC Cfr13I 1447 GGTCC G^G[ACGT]CC DraII 1446 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO109I 1446 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 1561 GAATTC G^AATTC FbaI 569 TGATCA T^GATCA HgaI 1388 GACGC GACGC(5/10) HindIII 760 AAGCTT A^AGCTT HpaI 1365 GTTAAC GTT^AAC MaeII 1691 ACGT A^CGT MamI 1696 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 335 CAATTG C^AATTG MunI 335 CAATTG C^AATTG NlaIV 1440 GGATCC GG[ACGT]^[ACGT]CC PflFI 1142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 485 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1446 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 933 ATTAAT AT^TAAT PstI 910 CTGCAG CTGCA^G SacI 500 GAGCTC GAGCT^C Sau96I 1447 GGTCC G^G[ACGT]CC SexAI 182 ACCTGGT A^CC[AT]GGT SfcI 910 CTGCAG C^T[GA][CT]AG SmlI 719 CTCAAG C^T[CT][GA]AG SspI 388 AATATT AAT^ATT Tth111I 1142 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 485 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Asp700I 1165 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1557 GAAAGAATTC BanII 500 GAGCTC G[GA]GC[CT]^C 690 GAGCCC BbvI 451 GCAGC GCAGC(8/12) 1324 GCTGC BmyI 500 GAGCTC G[AGT]GC[ACT]^C 690 GAGCCC Bsp1286I 500 GAGCTC G[AGT]GC[ACT]^C 690 GAGCCC BspHI 53 TCATGA T^CATGA 846 TCATGA BsrDI 1267 CATTGC GCAATG(2/0) 1732 CATTGC BstF5I 1174 CATCC GGATG(2/0) 1155 GGATG EcoT14I 18 CCTTGG C^C[AT][AT]GG 1058 CCAAGG Fnu4HI 451 GCAGC GC^[ACGT]GC 1324 GCTGC FokI 1174 CATCC GGATG(9/13) 1155 GGATG HinfI 1648 GAGTC G^A[ACGT]TC 1663 GAGTC HphI 661 TCACC GGTGA(8/7) 1350 TCACC ItaI 451 GCAGC GC^[ACGT]GC 1324 GCTGC MslI 573 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1351 CACCCCAGTG PleI 1648 GAGTC GAGTC(4/5) 1663 GAGTC RcaI 53 TCATGA T^CATGA 846 TCATGA SfaNI 1156 GATGC GCATC(5/9) 843 GCATC StyI 18 CCTTGG C^C[AT][AT]GG 1058 CCAAGG TaqI 330 TCGA T^CGA 369 TCGA TseI 451 GCAGC G^C[AT]GC 1324 GCTGC TthHB8I 330 TCGA T^CGA 369 TCGA XmnI 1165 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1557 GAAAGAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AcsI AluI AlwI ApoI BalI BfaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI BstYI DdeI DpnII EaeI EarI EcoRII EcoRV HaeIII HincII HindII Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MvaI NdeII NlaIII Sau3AI ScrFI Tru9I Tsp45I Tsp509I TspRI XhoII