(Circular) (Six-base) MAPSORT of: pFN21AE0984.seq Check: 5430 from: 1 to: 6481 pFN21AE0984 6481 bp With 182 enzymes: SgfI * March 28, 2012 13:20 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3824 1937 Size: 1887 4594 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3665 4544 5928 6196 1632 Size: 2033 879 1384 268 1917 Fragments arranged by size: 2033 1917 1384 879 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 5380 6357 1203 Size: 4177 977 1327 Fragments arranged by size: 4177 1327 977 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 5210 5210 Size: 6481 AleI CACnn'nnGTG Cuts at: 1166 3360 1166 Size: 2194 4287 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AlwNI CAG_nnn'CTG Cuts at: 2462 4224 5796 2462 Size: 1762 1572 3147 Fragments arranged by size: 3147 1762 1572 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 2226 2243 2885 3541 3566 3751 3790 4016 Size: 733 17 642 656 25 185 39 226 Cuts at: 4016 4168 6277 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 733 656 642 226 185 152 39 25 17 AseI AT'TA_AT Cuts at: 160 1032 2939 5035 160 Size: 872 1907 2096 1606 Fragments arranged by size: 2096 1907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 3806 1870 Size: 73 1863 4545 Fragments arranged by size: 4545 1863 73 BamHI G'GATC_C Cuts at: 3445 3811 4178 3445 Size: 366 367 5748 Fragments arranged by size: 5748 367 366 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3802 5257 618 Size: 325 217 627 2015 1455 1842 Fragments arranged by size: 2015 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2509 2699 3800 1391 Size: 1118 190 1101 4072 Fragments arranged by size: 4072 1118 1101 190 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5510 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4672 4706 1903 Size: 34 2735 34 3678 Fragments arranged by size: 3678 2735 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2413 4373 5583 2413 Size: 1960 1210 3311 Fragments arranged by size: 3311 1960 1210 BclI T'GATC_A Cuts at: 1548 2574 1548 Size: 1026 5455 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5092 136 Size: 107 122 71 631 605 3420 1525 Fragments arranged by size: 3420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3551 6476 3551 Size: 2925 3556 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 4542 5698 1790 Size: 2752 1156 2573 Fragments arranged by size: 2752 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2091 2401 3354 Size: 416 53 83 399 24 674 310 953 Cuts at: 3354 4212 5170 442 Size: 858 958 1753 Fragments arranged by size: 1753 958 953 858 674 416 399 310 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3174 5141 1239 Size: 1935 1967 2579 Fragments arranged by size: 2579 1967 1935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2709 4606 5471 5769 2709 Size: 1897 865 298 3421 Fragments arranged by size: 3421 1897 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2110 5144 882 Size: 617 611 3034 2219 Fragments arranged by size: 3034 2219 617 611 BsaAI yAC'GTr Cuts at: 493 1204 4272 493 Size: 711 3068 2702 Fragments arranged by size: 3068 2702 711 BsaBI GATnn'nnATC Cuts at: 3705 6475 3705 Size: 2770 3711 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4288 4670 5305 275 Size: 53 83 186 936 2755 382 635 1451 Fragments arranged by size: 2755 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4914 5312 5586 5733 6144 4914 Size: 398 274 147 411 5251 Fragments arranged by size: 5251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3270 3300 1178 Size: 30 165 30 1867 30 4359 Fragments arranged by size: 4359 1867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3120 1186 Size: 1934 4547 BseYI C'CCAG_C Cuts at: 2708 3236 5684 6180 2708 Size: 528 2448 496 3009 Fragments arranged by size: 3009 2448 528 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5908 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3931 4692 5720 1540 Size: 275 186 1930 761 1028 2301 Fragments arranged by size: 2301 1930 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2509 4542 4627 5698 2509 Size: 2033 85 1071 3292 Fragments arranged by size: 3292 2033 1071 85 BsmI GAATG_Cn' Cuts at: 3992 4085 3992 Size: 93 6388 BsmBI CGTCTCn'nnnn_ Cuts at: 3385 3385 Size: 6481 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2509 2699 3800 4542 4627 5698 1391 Size: 399 719 190 1101 742 85 1071 2174 Fragments arranged by size: 2174 1101 1071 742 719 399 190 85 BspEI T'CCGG_A Cuts at: 6144 6144 Size: 6481 BspHI T'CATG_A Cuts at: 2058 2851 4370 6100 2058 Size: 793 1519 1730 2439 Fragments arranged by size: 2439 1730 1519 793 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3836 844 Size: 2992 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3271 3736 4976 5150 65 Size: 1183 2023 465 1240 174 1396 Fragments arranged by size: 2023 1396 1240 1183 465 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5125 5312 1700 Size: 110 3315 187 2869 Fragments arranged by size: 3315 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6481 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6481 BstBI TT'CG_AA Cuts at: 5285 5285 Size: 6481 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6481 BstYI r'GATC_y Cuts at: 1972 2328 3445 3551 3811 4178 4563 4580 6032 Size: 356 1117 106 260 367 385 17 1452 Cuts at: 6032 6118 6130 6476 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1117 385 367 356 346 260 106 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4990 Fragments arranged by size: 4990 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2159 487 Size: 683 56 933 4809 Fragments arranged by size: 4809 933 683 56 BtsI GCAGTG_nn' Cuts at: 3011 3997 4791 4811 3011 Size: 986 794 20 4681 Fragments arranged by size: 4681 986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 3785 3785 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2487 2586 Size: 54 1425 50 275 18 264 393 99 Cuts at: 2586 3733 3928 4817 6446 8 Size: 1147 195 889 1629 43 Fragments arranged by size: 1629 1425 1147 889 393 275 264 195 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3928 1537 Size: 275 2116 4090 Fragments arranged by size: 4090 2116 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2327 3097 3641 3752 4411 1353 Size: 974 770 544 111 659 3423 Fragments arranged by size: 3423 974 770 659 544 111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5068 5442 5588 387 Size: 1064 138 3479 374 146 1280 Fragments arranged by size: 3479 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2507 2507 Size: 6481 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3174 3665 4544 5141 5928 6196 1239 Size: 393 1542 491 879 597 787 268 1524 Fragments arranged by size: 1542 1524 879 787 597 491 393 268 EcoO109I rG'GnC_Cy Cuts at: 3452 3452 Size: 6481 EcoRI G'AATT_C Cuts at: 3566 3790 4168 3566 Size: 224 378 5879 Fragments arranged by size: 5879 378 224 EcoRV GAT'ATC Cuts at: 2554 2829 3112 2554 Size: 275 283 5923 Fragments arranged by size: 5923 283 275 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3164 3164 Size: 6481 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 5628 1678 Size: 3950 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2813 2845 3096 3128 5122 Size: 32 135 32 1241 32 251 32 1994 Cuts at: 5122 5154 5196 5228 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1994 1241 251 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2134 3155 3372 3825 4071 669 Size: 988 281 196 1021 217 453 246 3079 Fragments arranged by size: 3079 1021 988 453 281 246 217 196 HindIII A'AGCT_T Cuts at: 748 2765 748 Size: 2017 4464 HpaI GTT'AAC Cuts at: 3372 4071 3372 Size: 699 5782 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 2340 4080 2340 Size: 1740 4741 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5595 5779 1305 Size: 4290 184 2007 Fragments arranged by size: 4290 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 2489 2588 3735 10 Size: 54 1425 343 264 393 99 1147 2756 Fragments arranged by size: 2756 1425 1147 393 343 264 99 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2582 3360 4439 4798 4957 518 Size: 648 843 573 778 1079 359 159 2042 Fragments arranged by size: 2042 1079 843 778 648 573 359 159 MspA1I CmG'CkG Cuts at: 1606 3897 4574 5722 5967 1606 Size: 2291 677 1148 245 2120 Fragments arranged by size: 2291 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4990 Fragments arranged by size: 4990 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 3839 5384 1132 Size: 97 2610 1545 2229 Fragments arranged by size: 2610 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 2496 1108 Size: 1388 5093 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3452 3452 Size: 6481 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6481 PsiI TTA'TAA Cuts at: 4051 4051 Size: 6481 PstI C_TGCA'G Cuts at: 830 2919 3833 830 Size: 2089 914 3478 Fragments arranged by size: 3478 2089 914 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 SacI G_AGCT'C Cuts at: 2509 2509 Size: 6481 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SexAI A'CCwGG_T Cuts at: 2187 2187 Size: 6481 SfcI C'TryA_G Cuts at: 826 1045 2915 3829 4964 5645 5836 826 Size: 219 1870 914 1135 681 191 1471 Fragments arranged by size: 1870 1471 1135 914 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2724 4585 5486 5748 820 Size: 197 926 781 1861 901 262 1553 Fragments arranged by size: 1861 1553 926 901 781 262 197 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 2395 4405 5 Size: 47 2343 2010 2081 Fragments arranged by size: 2343 2081 2010 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6481 StyI C'CwwG_G Cuts at: 513 1065 2004 2023 3063 3920 513 Size: 552 939 19 1040 857 3074 Fragments arranged by size: 3074 1040 939 857 552 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3313 4508 4525 4678 4863 5308 325 Size: 1255 1733 1195 17 153 185 445 1498 Fragments arranged by size: 1733 1498 1255 1195 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4727 6439 96 Size: 274 80 33 51 1443 2750 1712 138 Fragments arranged by size: 2750 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2532 2564 2581 2613 3270 3302 6441 6473 2532 Size: 32 17 32 657 32 3139 32 2540 Fragments arranged by size: 3139 2540 657 32 32 32 32 17 Tth111I GACn'n_nGTC Cuts at: 1316 3150 1316 Size: 1834 4647 XbaI T'CTAG_A Cuts at: 3817 3817 Size: 6481 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6481 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 3174 3566 4610 3174 Size: 392 1044 5045 Fragments arranged by size: 5045 1044 392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI