################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbeI BbrPI BcgI BglI BlpI Bpu1102I BsaAI BsaHI BsiEI BsiWI BsrBI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII CpoI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MluI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I Tth111I XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccII 3223 CGCG CG^CG AccIII 3213 TCCGGA T^CCGGA AflII 1222 CTTAAG C^TTAAG AflIII 16 ACATGT A^C[GA][CT]GT AocI 2143 CCTGAGG CC^T[ACGT]AGG Aor51HI 3678 AGCGCT AGC^GCT AspHI 1085 GAGCTC G[AT]GC[AT]^C BciVI 2327 GGATAC GTATCC(6/5) BfrI 1222 CTTAAG C^TTAAG BseAI 3213 TCCGGA T^CCGGA BsiHKAI 1085 GAGCTC G[AT]GC[AT]^C BsmBI 2502 GAGACG CGTCTC(1/5) BspEI 3213 TCCGGA T^CCGGA BspLU11I 16 ACATGT A^CATGT BsrFI 788 GCCGGT [GA]^CCGG[CT] BssSI 1789 CACGAG CACGAG(-5/-1) BstAPI 3973 GCAATGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 3223 CGCG CG^CG Bsu36I 2143 CCTGAGG CC^T[ACGT]AGG Cfr10I 788 GCCGGT [GA]^CCGG[CT] DraI 2709 TTTAAA TTT^AAA DrdI 44 GACGAGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3678 AGCGCT AGC^GCT Eco81I 2143 CCTGAGG CC^T[ACGT]AGG EcoNI 409 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2629 ATGCAT ATGCA^T MroI 3213 TCCGGA T^CCGGA MspA1I 2680 CAGCTG C[AC]G^C[GT]G MvnI 3223 CGCG CG^CG NcoI 1277 CCATGG C^CATGG NheI 3033 GCTAGC G^CTAGC NsiI 2629 ATGCAT ATGCA^T PaeR7I 2877 CTCGAG C^TCGAG PflMI 391 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 2937 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 3859 CTGCAG CTGCA^G PvuII 2680 CAGCTG CAG^CTG SacI 1085 GAGCTC GAGCT^C ScaI 3479 AGTACT AGT^ACT SmaI 39 CCCGGG CCC^GGG SspI 1840 AATATT AAT^ATT StuI 3622 AGGCCT AGG^CCT SwaI 2708 ATTTAAAT ATTT^AAAT Van91I 391 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2877 CTCGAG C^TCGAG XmaCI 39 CCCGGG C^CCGGG XmaI 39 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### ApaI 1031 GGGCCC GGGCC^C 3870 GGGCCC AvrII 3417 CCTAGG C^CTAGG 3444 CCTAGG BbvCI 86 CCTCAGC CCTCAGC(-5/-2) 130 CCTCAGC BclI 1785 TGATCA T^GATCA 2730 TGATCA BglII 796 AGATCT A^GATCT 937 AGATCT BlnI 3417 CCTAGG C^CTAGG 3444 CCTAGG BsaBI 896 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2835 GATAAATATC BsaI 1180 GAGACC GGTCTC(1/5) 2357 GAGACC BsaWI 1721 ACCGGA [AT]^CCGG[AT] 3213 TCCGGA BsgI 2336 CTGCAC GTGCAG(16/14) 2124 GTGCAG BsmI 2206 GAATGC GAATGC(1/-1) 3379 GCATTC Bsp1407I 3683 TGTACA T^GTACA 3848 TGTACA BspDI 2260 ATCGAT AT^CGAT 3056 ATCGAT BsrGI 3683 TGTACA T^GTACA 3848 TGTACA BstXI 1125 CCAGAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3641 CCAAGACCTTGG BstYI 796 AGATCT [GA]^GATC[CT] 937 AGATCT ClaI 2260 ATCGAT AT^CGAT 3056 ATCGAT DsaI 1277 CCATGG C^C[GA][CT]GG 1393 CCGTGG FbaI 1785 TGATCA T^GATCA 2730 TGATCA HaeII 3630 GGCGCT [GA]GCGC^[CT] 3678 AGCGCT MaeII 1508 ACGT A^CGT 2049 ACGT MamI 896 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2835 GATAAATATC MfeI 3123 CAATTG C^AATTG 3338 CAATTG MflI 796 AGATCT [GA]^GATC[CT] 937 AGATCT MunI 3123 CAATTG C^AATTG 3338 CAATTG SfcI 1298 CTACAG C^T[GA][CT]AG 3859 CTGCAG SmlI 1222 CTTAAG C^T[CT][GA]AG 2877 CTCGAG SphI 2574 GCATGC GCATG^C 3584 GCATGC SspBI 3683 TGTACA T^GTACA 3848 TGTACA XhoII 796 AGATCT [GA]^GATC[CT] 937 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmnI