(Linear) (Six-base) MAPSORT of: KIEE9634.seq Check: 2071 from: 1 to: 4122 KIEE9634.orf With 182 enzymes: SgfI * January 14, 2011 16:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1517 2152 3778 4122 Size: 1517 635 1626 344 Fragments arranged by size: 1626 1517 635 344 AfeI AGC'GCT Cuts at: 0 3680 4122 Size: 3680 442 AflII C'TTAA_G Cuts at: 0 1222 4122 Size: 1222 2900 AflIII A'CryG_T Cuts at: 0 16 4122 Size: 16 4106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2213 2223 4122 Size: 2213 10 1899 Fragments arranged by size: 2213 1899 10 AlwNI CAG_nnn'CTG Cuts at: 0 397 1475 1495 2451 4122 Size: 397 1078 20 956 1671 Fragments arranged by size: 1671 1078 956 397 20 ApaI G_GGCC'C Cuts at: 0 1035 3874 4122 Size: 1035 2839 248 Fragments arranged by size: 2839 1035 248 ApoI r'AATT_y Cuts at: 0 1339 1914 2712 2742 2762 2905 2929 3439 Size: 1339 575 798 30 20 143 24 510 Cuts at: 3439 4122 Size: 683 Fragments arranged by size: 1339 798 683 575 510 143 30 24 20 AvaI C'yCGr_G Cuts at: 0 39 56 1174 2877 4122 Size: 39 17 1118 1703 1245 Fragments arranged by size: 1703 1245 1118 39 17 AvrII C'CTAG_G Cuts at: 0 3417 3444 4122 Size: 3417 27 678 Fragments arranged by size: 3417 678 27 BanI G'GyrC_C Cuts at: 0 323 402 695 2139 4122 Size: 323 79 293 1444 1983 Fragments arranged by size: 1983 1444 323 293 79 BanII G_rGCy'C Cuts at: 0 1035 1089 1175 3874 4122 Size: 1035 54 86 2699 248 Fragments arranged by size: 2699 1035 248 86 54 BbsI GAAGACnn'nnnn_ Cuts at: 0 783 2015 4115 4122 Size: 783 1232 2100 7 Fragments arranged by size: 2100 1232 783 7 BbvCI CC'TCA_GC Cuts at: 0 87 131 4122 Size: 87 44 3991 Fragments arranged by size: 3991 87 44 BciVI GTATCCnnnnn_n' Cuts at: 0 2321 4122 Size: 2321 1801 BclI T'GATC_A Cuts at: 0 1785 2730 4122 Size: 1785 945 1392 Fragments arranged by size: 1785 1392 945 BfrBI ATG'CAT Cuts at: 0 2631 4122 Size: 2631 1491 BglII A'GATC_T Cuts at: 0 796 937 4122 Size: 796 141 3185 Fragments arranged by size: 3185 796 141 Bme1580I G_kGCm'C Cuts at: 0 174 405 1035 3874 4122 Size: 174 231 630 2839 248 Fragments arranged by size: 2839 630 248 231 174 BmrI ACTGGGnnnn_n' Cuts at: 0 328 385 533 575 641 4122 Size: 328 57 148 42 66 3481 Fragments arranged by size: 3481 328 148 66 57 42 BmtI G_CTAG'C Cuts at: 0 3037 4122 Size: 3037 1085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 455 1063 1251 1679 2203 3190 3287 Size: 182 273 608 188 428 524 987 97 Cuts at: 3287 4122 Size: 835 Fragments arranged by size: 987 835 608 524 428 273 188 182 97 Bpu10I CC'TnA_GC Cuts at: 0 87 131 4122 Size: 87 44 3991 Fragments arranged by size: 3991 87 44 BsaI GGTCTCn'nnnn_ Cuts at: 0 1174 2351 4122 Size: 1174 1177 1771 Fragments arranged by size: 1771 1177 1174 BsaBI GATnn'nnATC Cuts at: 0 900 2839 4122 Size: 900 1939 1283 Fragments arranged by size: 1939 1283 900 BsaWI w'CCGG_w Cuts at: 0 1721 3213 4122 Size: 1721 1492 909 Fragments arranged by size: 1721 1492 909 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 939 969 1249 1279 2170 2200 4122 Size: 939 30 280 30 891 30 1922 Fragments arranged by size: 1922 939 891 280 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 80 1192 1216 1360 1363 2161 2164 2191 Size: 80 1112 24 144 3 798 3 27 Cuts at: 2191 3589 4122 Size: 1398 533 Fragments arranged by size: 1398 1112 798 533 144 80 27 24 3 3 BseYI C'CCAG_C Cuts at: 0 3579 3788 4122 Size: 3579 209 334 Fragments arranged by size: 3579 334 209 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2145 2321 4122 Size: 2145 176 1801 Fragments arranged by size: 2145 1801 176 BsiHKAI G_wGCw'C Cuts at: 0 1089 4122 Size: 1089 3033 BsmI GAATG_Cn' Cuts at: 0 2212 3379 4122 Size: 2212 1167 743 Fragments arranged by size: 2212 1167 743 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2496 4122 Size: 2496 1626 Bsp1286I G_dGCh'C Cuts at: 0 174 405 1035 1089 1175 3874 4122 Size: 174 231 630 54 86 2699 248 Fragments arranged by size: 2699 630 248 231 174 86 54 BspEI T'CCGG_A Cuts at: 0 3213 4122 Size: 3213 909 BspHI T'CATG_A Cuts at: 0 431 1554 3507 3719 4122 Size: 431 1123 1953 212 403 Fragments arranged by size: 1953 1123 431 403 212 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 477 984 3227 4122 Size: 477 507 2243 895 Fragments arranged by size: 2243 895 507 477 BsrDI GCAATG_nn' Cuts at: 0 2585 3122 3980 4122 Size: 2585 537 858 142 Fragments arranged by size: 2585 858 537 142 BsrFI r'CCGG_y Cuts at: 0 788 4122 Size: 788 3334 BsrGI T'GTAC_A Cuts at: 0 3683 3848 4122 Size: 3683 165 274 Fragments arranged by size: 3683 274 165 BssSI C'ACGA_G Cuts at: 0 1789 4122 Size: 1789 2333 BstAPI GCAn_nnn'nTGC Cuts at: 0 3979 4122 Size: 3979 143 BstXI CCAn_nnnn'nTGG Cuts at: 0 1132 3648 4122 Size: 1132 2516 474 Fragments arranged by size: 2516 1132 474 BstYI r'GATC_y Cuts at: 0 796 937 4122 Size: 796 141 3185 Fragments arranged by size: 3185 796 141 Bsu36I CC'TnA_GG Cuts at: 0 2144 4122 Size: 2144 1978 BtgI C'CryG_G Cuts at: 0 1277 1393 4122 Size: 1277 116 2729 Fragments arranged by size: 2729 1277 116 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1042 4122 Size: 1042 3080 BtsI GCAGTG_nn' Cuts at: 0 3805 4122 Size: 3805 317 ClaI AT'CG_AT Cuts at: 0 2261 3057 4122 Size: 2261 796 1065 Fragments arranged by size: 2261 1065 796 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2359 2394 4122 Size: 2359 35 1728 Fragments arranged by size: 2359 1728 35 DraI TTT'AAA Cuts at: 0 2711 4122 Size: 2711 1411 DrdI GACnn_nn'nnGTC Cuts at: 0 50 4122 Size: 50 4072 EaeI y'GGCC_r Cuts at: 0 2684 2856 3119 4122 Size: 2684 172 263 1003 Fragments arranged by size: 2684 1003 263 172 EarI CTCTTCn'nnn_ Cuts at: 0 281 1075 1658 1892 2377 4122 Size: 281 794 583 234 485 1745 Fragments arranged by size: 1745 794 583 485 281 234 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3667 4122 Size: 3667 455 EcoICRI GAG'CTC Cuts at: 0 1087 4122 Size: 1087 3035 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 455 1063 1251 1517 1679 2152 2203 Size: 182 273 608 188 266 162 473 51 Cuts at: 2203 3190 3287 3778 4122 Size: 987 97 491 344 Fragments arranged by size: 987 608 491 473 344 273 266 188 182 162 97 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 413 4122 Size: 413 3709 EcoO109I rG'GnC_Cy Cuts at: 0 104 271 659 914 1032 1996 4122 Size: 104 167 388 255 118 964 2126 Fragments arranged by size: 2126 964 388 255 167 118 104 EcoRI G'AATT_C Cuts at: 0 2742 2762 2905 3439 4122 Size: 2742 20 143 534 683 Fragments arranged by size: 2742 683 534 143 20 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 191 292 1234 1237 4122 Size: 191 101 942 3 2885 Fragments arranged by size: 2885 942 191 101 3 HaeII r_GCGC'y Cuts at: 0 3634 3682 4122 Size: 3634 48 440 Fragments arranged by size: 3634 440 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 426 458 643 675 1038 1070 1441 1473 Size: 426 32 185 32 363 32 371 32 Cuts at: 1473 3185 3217 3769 3801 3999 4031 4122 Size: 1712 32 552 32 198 32 91 Fragments arranged by size: 1712 552 426 371 363 198 185 91 32 32 32 32 32 32 32 MfeI C'AATT_G Cuts at: 0 3123 3338 4122 Size: 3123 215 784 Fragments arranged by size: 3123 784 215 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1093 1287 2882 3282 4122 Size: 1093 194 1595 400 840 Fragments arranged by size: 1595 1093 840 400 194 MscI TGG'CCA Cuts at: 0 2686 2858 3121 4122 Size: 2686 172 263 1001 Fragments arranged by size: 2686 1001 263 172 MslI CAynn'nnrTG Cuts at: 0 683 710 2806 4122 Size: 683 27 2096 1316 Fragments arranged by size: 2096 1316 683 27 MspA1I CmG'CkG Cuts at: 0 2682 4122 Size: 2682 1440 NcoI C'CATG_G Cuts at: 0 1277 4122 Size: 1277 2845 NheI G'CTAG_C Cuts at: 0 3033 4122 Size: 3033 1089 NsiI A_TGCA'T Cuts at: 0 2633 4122 Size: 2633 1489 NspI r_CATG'y Cuts at: 0 20 1828 2578 2805 2811 3115 3588 4122 Size: 20 1808 750 227 6 304 473 534 Fragments arranged by size: 1808 750 534 473 304 227 20 6 PasI CC'CwG_GG Cuts at: 0 397 2233 4122 Size: 397 1836 1889 Fragments arranged by size: 1889 1836 397 PciI A'CATG_T Cuts at: 0 16 4122 Size: 16 4106 PflMI CCAn_nnn'nTGG Cuts at: 0 397 4122 Size: 397 3725 PfoI T'CCnGG_A Cuts at: 0 408 1656 4122 Size: 408 1248 2466 Fragments arranged by size: 2466 1248 408 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 810 842 1138 1170 4122 Size: 810 32 296 32 2952 Fragments arranged by size: 2952 810 296 32 32 PpuMI rG'GwC_Cy Cuts at: 0 659 914 1996 4122 Size: 659 255 1082 2126 Fragments arranged by size: 2126 1082 659 255 PshAI GACnn'nnGTC Cuts at: 0 2941 4122 Size: 2941 1181 PspOMI G'GGCC_C Cuts at: 0 1031 3870 4122 Size: 1031 2839 252 Fragments arranged by size: 2839 1031 252 PspXI vC'TCGA_Gb Cuts at: 0 2877 4122 Size: 2877 1245 PstI C_TGCA'G Cuts at: 0 3863 4122 Size: 3863 259 PvuII CAG'CTG Cuts at: 0 2682 4122 Size: 2682 1440 SacI G_AGCT'C Cuts at: 0 1089 4122 Size: 1089 3033 ScaI AGT'ACT Cuts at: 0 3481 4122 Size: 3481 641 SfcI C'TryA_G Cuts at: 0 1298 3859 4122 Size: 1298 2561 263 Fragments arranged by size: 2561 1298 263 SmaI CCC'GGG Cuts at: 0 41 4122 Size: 41 4081 SmlI C'TyrA_G Cuts at: 0 1222 2877 4122 Size: 1222 1655 1245 Fragments arranged by size: 1655 1245 1222 SphI G_CATG'C Cuts at: 0 2578 3588 4122 Size: 2578 1010 534 Fragments arranged by size: 2578 1010 534 SspI AAT'ATT Cuts at: 0 1842 4122 Size: 1842 2280 StuI AGG'CCT Cuts at: 0 3624 4122 Size: 3624 498 StyI C'CwwG_G Cuts at: 0 1277 2532 2853 3417 3444 3563 3647 4122 Size: 1277 1255 321 564 27 119 84 475 Fragments arranged by size: 1277 1255 564 475 321 119 84 27 SwaI ATTT'AAAT Cuts at: 0 2711 4122 Size: 2711 1411 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 264 722 824 1562 2902 3266 4122 Size: 264 458 102 738 1340 364 856 Fragments arranged by size: 1340 856 738 458 364 264 102 TatI w'GTAC_w Cuts at: 0 2111 2951 3479 3683 3848 4040 4122 Size: 2111 840 528 204 165 192 82 Fragments arranged by size: 2111 840 528 204 192 165 82 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2069 2101 2170 2202 4122 Size: 2069 32 69 32 1920 Fragments arranged by size: 2069 1920 69 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1275 1284 1591 3462 3624 4122 Size: 1275 9 307 1871 162 498 Fragments arranged by size: 1871 1275 498 307 162 9 XhoI C'TCGA_G Cuts at: 0 2877 4122 Size: 2877 1245 XmaI C'CCGG_G Cuts at: 0 39 4122 Size: 39 4083 XmnI GAAnn'nnTTC Cuts at: 0 1217 3707 3751 4024 4122 Size: 1217 2490 44 273 98 Fragments arranged by size: 2490 1217 273 98 44 Enzymes that do cut: AcuI AfeI AflII AflIII AlfI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BbvCI BciVI BclI BfrBI BglII Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I MfeI MmeI MscI MslI MspA1I NcoI NheI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI ScaI SfcI SmaI SmlI SphI SspI StuI StyI SwaI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BcgI BglI BlpI BmgBI BplI BpuEI BsaAI BsaHI BsiEI BsiWI BsrBI BssHII BstBI BstEII BstZ17I DraIII EagI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NotI NruI PacI PmeI PmlI PsiI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI Tth111I XbaI ZraI