################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII BalI BanII BbrPI BbvCI BcgI BciVI BclI BfrI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssSI BstBI BstEII BstPI Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII KpnI Ksp632I MaeII MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 579 GTATAC GT^[AC][GT]AC AcsI 945 GAATTT [GA]^AATT[CT] Aor51HI 929 AGCGCT AGC^GCT ApoI 945 GAATTT [GA]^AATT[CT] AvrII 747 CCTAGG C^CTAGG BamHI 751 GGATCC G^GATCC BglI 17 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 747 CCTAGG C^CTAGG BmyI 478 GGGCAC G[AGT]GC[ACT]^C BseRI 784 CTCCTC GAGGAG(10/8) BsiEI 293 CGGCCG CG[GA][CT]^CG BsmBI 418 GAGACG CGTCTC(1/5) Bsp1286I 478 GGGCAC G[AGT]GC[ACT]^C BspHI 897 TCATGA T^CATGA BspMI 143 ACCTGC ACCTGC(4/8) Bst1107I 579 GTATAC GTA^TAC BstAPI 509 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 468 CCAGCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 579 GTATAC GTA^TAC DraII 1141 AGGCCCC [GA]G^G[ACGT]CC[CT] EagI 293 CGGCCG C^GGCCG EclXI 293 CGGCCG C^GGCCG Eco47III 929 AGCGCT AGC^GCT Eco52I 293 CGGCCG C^GGCCG EcoO109I 1141 AGGCCCC [GA]G^G[ACGT]CC[CT] HincII 1083 GTTAAC GT[CT]^[GA]AC HindII 1083 GTTAAC GT[CT]^[GA]AC HpaI 1083 GTTAAC GTT^AAC PflMI 279 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 589 CAGCTG CAG^CTG RcaI 897 TCATGA T^CATGA SmaI 355 CCCGGG CCC^GGG TaqI 989 TCGA T^CGA TthHB8I 989 TCGA T^CGA Van91I 279 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 355 CCCGGG C^CCGGG XmaI 355 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BbeI 320 GGCGCC GGCGC^C 400 GGCGCC BbsI 1150 GAAGAC GAAGAC(2/6) 1011 GTCTTC BglII 512 AGATCT A^GATCT 842 AGATCT BpuAI 1150 GAAGAC GAAGAC(2/6) 1011 GTCTTC BsgI 225 GTGCAG GTGCAG(16/14) 432 GTGCAG BssHII 180 GCGCGC G^CGCGC 471 GCGCGC DdeI 365 CTGAG C^T[ACGT]AG 1079 CTCAG EcoNI 324 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1088 CCTCACCAAGG EcoT14I 747 CCTAGG C^C[AT][AT]GG 1093 CCAAGG HphI 538 GGTGA GGTGA(8/7) 1090 TCACC KasI 320 GGCGCC G^GCGCC 400 GGCGCC MaeIII 775 GTGAC ^GT[ACGT]AC 1195 GTGAC MslI 669 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 952 CACTGGGATG NarI 320 GGCGCC GG^CGCC 400 GGCGCC PstI 265 CTGCAG CTGCA^G 908 CTGCAG SfcI 265 CTGCAG C^T[GA][CT]AG 908 CTGCAG SfoI 320 GGCGCC GGC^GCC 400 GGCGCC StyI 747 CCTAGG C^C[AT][AT]GG 1093 CCAAGG Tsp45I 775 GTGAC ^GT[GC]AC 1195 GTGAC Tsp509I 946 AATT ^AATT 967 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaI AvaII BanI BbvI BcnI BfaI BsaHI BsaI BsaJI BsiYI BslI BsmAI BsmFI BsoBI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KspI MaeI MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI SacII Sau3AI Sau96I ScrFI Tru9I TseI TspRI XhoII