(Circular) (Six-base) MAPSORT of: pFN21AE4076.seq Check: 7311 from: 1 to: 5911 pFN21AE4076 5911 bp With 182 enzymes: SgfI * March 28, 2012 13:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2585 3254 1937 Size: 648 669 4594 Fragments arranged by size: 4594 669 648 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2788 3189 3974 5358 5626 1632 Size: 1156 401 785 1384 268 1917 Fragments arranged by size: 1917 1384 1156 785 401 268 AfeI AGC'GCT Cuts at: 2936 2936 Size: 5911 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5714 AflIII A'CryG_T Cuts at: 1203 4810 5787 1203 Size: 3607 977 1327 Fragments arranged by size: 3607 1327 977 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 3654 5226 3654 Size: 1572 4339 ApaLI G'TGCA_C Cuts at: 3968 5124 3968 Size: 1156 4755 ApoI r'AATT_y Cuts at: 1493 2950 3220 3446 3598 5707 1493 Size: 1457 270 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 270 226 152 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2171 2180 2360 2420 3178 3236 1870 Size: 73 228 9 180 60 758 58 4545 Fragments arranged by size: 4545 758 228 180 73 60 58 9 AvrII C'CTAG_G Cuts at: 2752 2752 Size: 5911 BamHI G'GATC_C Cuts at: 2756 3241 3608 2756 Size: 485 367 5059 Fragments arranged by size: 5059 485 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2325 2405 2484 3232 4687 Size: 325 217 627 538 80 79 748 1455 Cuts at: 4687 618 Size: 1842 Fragments arranged by size: 1842 1455 748 627 538 325 217 80 79 BanII G_rGCy'C Cuts at: 1391 3230 1391 Size: 1839 4072 BbeI G_GCGC'C Cuts at: 2329 2409 2329 Size: 80 5831 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3009 3162 928 Size: 971 1110 153 3677 Fragments arranged by size: 3677 1110 971 153 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4102 4136 1903 Size: 34 2165 34 3678 Fragments arranged by size: 3678 2165 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 1548 Size: 5911 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2028 4522 136 Size: 107 122 71 631 605 356 2494 1525 Fragments arranged by size: 2494 1525 631 605 356 122 107 71 BglII A'GATC_T Cuts at: 2517 2847 5906 2517 Size: 330 3059 2522 Fragments arranged by size: 3059 2522 330 BlpI GC'TnA_GC Cuts at: 3328 3328 Size: 5911 Bme1580I G_kGCm'C Cuts at: 1790 2487 3972 5128 1790 Size: 697 1485 1156 2573 Fragments arranged by size: 2573 1485 1156 697 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2968 3642 4600 Size: 416 53 83 399 24 1551 674 958 Cuts at: 4600 442 Size: 1753 Fragments arranged by size: 1753 1551 958 674 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5911 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2446 2446 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4571 1239 Size: 3332 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4901 5199 4036 Size: 865 298 4748 Fragments arranged by size: 4748 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2168 2406 2897 4574 882 Size: 617 669 238 491 1677 2219 Fragments arranged by size: 2219 1677 669 617 491 238 BsaAI yAC'GTr Cuts at: 493 1204 3702 493 Size: 711 2498 2702 Fragments arranged by size: 2702 2498 711 BsaBI GATnn'nnATC Cuts at: 5905 5905 Size: 5911 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2061 2102 2326 2406 Size: 53 83 186 936 528 41 224 80 Cuts at: 2406 3718 4100 4735 275 Size: 1312 382 635 1451 Fragments arranged by size: 1451 1312 936 635 528 382 224 186 83 80 53 41 BsaWI w'CCGG_w Cuts at: 4344 4742 5016 5163 5574 4344 Size: 398 274 147 411 4681 Fragments arranged by size: 4681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2901 2931 1178 Size: 30 165 30 1498 30 4158 Fragments arranged by size: 4158 1498 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2780 1186 Size: 1594 4317 BseYI C'CCAG_C Cuts at: 2480 5114 5610 2480 Size: 2634 496 2781 Fragments arranged by size: 2781 2634 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2251 2458 1163 Size: 573 515 207 4616 Fragments arranged by size: 4616 573 515 207 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2301 3361 4122 5150 1540 Size: 275 186 300 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 300 275 186 BsiHKAI G_wGCw'C Cuts at: 3972 4057 5128 3972 Size: 85 1071 4755 Fragments arranged by size: 4755 1071 85 BsmI GAATG_Cn' Cuts at: 3422 3515 3422 Size: 93 5818 BsmBI CGTCTCn'nnnn_ Cuts at: 2417 2417 Size: 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2487 3230 3972 4057 5128 1391 Size: 399 697 743 742 85 1071 2174 Fragments arranged by size: 2174 1071 743 742 697 399 85 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 2902 3800 5530 2902 Size: 898 1730 3283 Fragments arranged by size: 3283 1730 898 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2157 3266 844 Size: 1313 1109 3489 Fragments arranged by size: 3489 1313 1109 BsrBI CCG'CTC Cuts at: 1394 1823 2055 2240 2398 3798 1394 Size: 429 232 185 158 1400 3507 Fragments arranged by size: 3507 1400 429 232 185 158 BsrDI GCAATG_nn' Cuts at: 65 1248 4406 4580 65 Size: 1183 3158 174 1396 Fragments arranged by size: 3158 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2119 2279 2300 2375 4555 4742 1700 Size: 110 309 160 21 75 2180 187 2869 Fragments arranged by size: 2869 2180 309 187 160 110 75 21 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5911 BssHII G'CGCG_C Cuts at: 1925 2185 2476 1925 Size: 260 291 5360 Fragments arranged by size: 5360 291 260 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 2520 794 Size: 1726 4185 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstXI CCAn_nnnn'nTGG Cuts at: 2480 2480 Size: 5911 BstYI r'GATC_y Cuts at: 1972 2517 2756 2847 2875 3241 3608 3993 4010 Size: 545 239 91 28 366 367 385 17 Cuts at: 4010 5462 5548 5560 5906 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 545 385 367 366 346 239 91 86 28 17 12 BstZ17I GTA'TAC Cuts at: 2586 2586 Size: 5911 BtgI C'CryG_G Cuts at: 513 1065 2023 2133 2190 2225 513 Size: 552 958 110 57 35 4199 Fragments arranged by size: 4199 958 552 110 57 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 2464 2823 3427 4221 4241 2464 Size: 359 604 794 20 4134 Fragments arranged by size: 4134 794 604 359 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2020 2281 2298 Size: 54 1425 50 275 18 190 261 17 Cuts at: 2298 3358 4247 5876 8 Size: 1060 889 1629 43 Fragments arranged by size: 1629 1425 1060 889 275 261 190 54 50 43 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2298 3358 1537 Size: 275 486 1060 4090 Fragments arranged by size: 4090 1060 486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3841 1353 Size: 2488 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 2247 4498 4872 5018 387 Size: 1064 138 474 184 2251 374 146 1280 Fragments arranged by size: 2251 1280 1064 474 374 184 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2788 3189 3974 4571 5358 5626 1239 Size: 393 1156 401 785 597 787 268 1524 Fragments arranged by size: 1524 1156 787 785 597 401 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2333 3097 2333 Size: 764 5147 EcoO109I rG'GnC_Cy Cuts at: 3147 3147 Size: 5911 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2539 3048 2539 Size: 509 5402 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 2329 2409 2938 5058 1678 Size: 651 80 529 2120 2531 Fragments arranged by size: 2531 2120 651 529 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3161 3193 4552 4584 4626 Size: 32 135 32 1589 32 1359 32 42 Cuts at: 4626 4658 1373 Size: 32 2626 Fragments arranged by size: 2626 1589 1359 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3255 3501 669 Size: 988 281 1152 165 246 3079 Fragments arranged by size: 3079 1152 988 281 246 165 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3090 3501 3090 Size: 411 5500 KasI G'GCGC_C Cuts at: 2325 2405 2325 Size: 80 5831 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5025 5209 1305 Size: 3720 184 2007 Fragments arranged by size: 3720 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4089 Fragments arranged by size: 4089 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2678 2961 3869 4228 4387 518 Size: 648 1512 283 908 359 159 2042 Fragments arranged by size: 2042 1512 908 648 359 283 159 MspA1I CmG'CkG Cuts at: 1606 2135 2158 2192 2227 2596 3327 4004 5152 Size: 529 23 34 35 369 731 677 1148 Cuts at: 5152 5397 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 731 677 529 369 245 35 34 23 NaeI GCC'GGC Cuts at: 2121 2281 2302 2377 2121 Size: 160 21 75 5655 Fragments arranged by size: 5655 160 75 21 NarI GG'CG_CC Cuts at: 2326 2406 2326 Size: 80 5831 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5359 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NgoMIV G'CCGG_C Cuts at: 2119 2279 2300 2375 2119 Size: 160 21 75 5655 Fragments arranged by size: 5655 160 75 21 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5911 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 3269 4814 1132 Size: 97 2040 1545 2229 Fragments arranged by size: 2229 2040 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5911 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 2290 1108 Size: 1182 4729 PfoI T'CCnGG_A Cuts at: 2331 2630 2331 Size: 299 5612 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5911 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PstI C_TGCA'G Cuts at: 830 2274 2917 3263 830 Size: 1444 643 346 3478 Fragments arranged by size: 3478 1444 643 346 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 PvuII CAG'CTG Cuts at: 2596 2596 Size: 5911 SacII CC_GC'GG Cuts at: 2136 2193 2228 2136 Size: 57 35 5819 Fragments arranged by size: 5819 57 35 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 2270 2913 3259 4394 5075 5266 826 Size: 219 1225 643 346 1135 681 191 1471 Fragments arranged by size: 1471 1225 1135 681 643 346 219 191 SfoI GGC'GCC Cuts at: 2327 2407 2327 Size: 80 5831 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 2362 3238 1872 Size: 490 876 4545 Fragments arranged by size: 4545 876 490 SmlI C'TyrA_G Cuts at: 820 1017 1943 4015 4916 5178 820 Size: 197 926 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5911 StyI C'CwwG_G Cuts at: 513 1065 2752 3098 3350 513 Size: 552 1687 346 252 3074 Fragments arranged by size: 3074 1687 552 346 252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3093 3938 3955 4108 4293 4738 325 Size: 1255 1513 845 17 153 185 445 1498 Fragments arranged by size: 1513 1498 1255 845 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4157 5869 96 Size: 274 80 33 51 1443 2180 1712 138 Fragments arranged by size: 2180 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2867 2899 5871 5903 2867 Size: 32 2972 32 2875 Fragments arranged by size: 2972 2875 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 2360 3236 1870 Size: 490 876 4545 Fragments arranged by size: 4545 876 490 XmnI GAAnn'nnTTC Cuts at: 4040 4040 Size: 5911 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbvCI BfrBI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI