Restriction map for
pFN21AE4076
(Linear) (Six-base) MAPSORT of: KIEE4076.seq Check: 7369 from: 1 to: 1206
KIEE4076.orf
With 182 enzymes: SgfI *
March 22, 2012 17:19 ..
AccI GT'mk_AC
Cuts at: 0 580 1206
Size: 580 626
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 1184 1206
Size: 783 401 22
Fragments arranged by size:
783 401 22
AfeI AGC'GCT
Cuts at: 0 931 1206
Size: 931 275
ApoI r'AATT_y
Cuts at: 0 945 1206
Size: 945 261
AvaI C'yCGr_G
Cuts at: 0 166 175 355 415 1173 1206
Size: 166 9 180 60 758 33
Fragments arranged by size:
758 180 166 60 33 9
AvrII C'CTAG_G
Cuts at: 0 747 1206
Size: 747 459
BamHI G'GATC_C
Cuts at: 0 751 1206
Size: 751 455
BanI G'GyrC_C
Cuts at: 0 320 400 479 1206
Size: 320 80 79 727
Fragments arranged by size:
727 320 80 79
BbeI G_GCGC'C
Cuts at: 0 324 404 1206
Size: 324 80 802
Fragments arranged by size:
802 324 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 1004 1157 1206
Size: 1004 153 49
Fragments arranged by size:
1004 153 49
BglI GCCn_nnn'nGGC
Cuts at: 0 23 1206
Size: 23 1183
BglII A'GATC_T
Cuts at: 0 512 842 1206
Size: 512 330 364
Fragments arranged by size:
512 364 330
Bme1580I G_kGCm'C
Cuts at: 0 482 1206
Size: 482 724
BmrI ACTGGGnnnn_n'
Cuts at: 0 963 1206
Size: 963 243
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 441 1206
Size: 441 765
BsaI GGTCTCn'nnnn_
Cuts at: 0 163 401 892 1206
Size: 163 238 491 314
Fragments arranged by size:
491 314 238 163
BsaHI Gr'CG_yC
Cuts at: 0 56 97 321 401 1206
Size: 56 41 224 80 805
Fragments arranged by size:
805 224 80 56 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 896 926 1206
Size: 896 30 280
Fragments arranged by size:
896 280 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 1206
Size: 775 431
BseYI C'CCAG_C
Cuts at: 0 475 1206
Size: 475 731
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 453 1206
Size: 246 207 753
Fragments arranged by size:
753 246 207
BsiEI CG_ry'CG
Cuts at: 0 296 1206
Size: 296 910
BsmBI CGTCTCn'nnnn_
Cuts at: 0 412 1206
Size: 412 794
Bsp1286I G_dGCh'C
Cuts at: 0 482 1206
Size: 482 724
BspHI T'CATG_A
Cuts at: 0 897 1206
Size: 897 309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 1206
Size: 152 1054
BsrBI CCG'CTC
Cuts at: 0 50 235 393 1206
Size: 50 185 158 813
Fragments arranged by size:
813 185 158 50
BsrFI r'CCGG_y
Cuts at: 0 114 274 295 370 1206
Size: 114 160 21 75 836
Fragments arranged by size:
836 160 114 75 21
BssHII G'CGCG_C
Cuts at: 0 180 471 1206
Size: 180 291 735
Fragments arranged by size:
735 291 180
BstAPI GCAn_nnn'nTGC
Cuts at: 0 515 1206
Size: 515 691
BstXI CCAn_nnnn'nTGG
Cuts at: 0 475 1206
Size: 475 731
BstYI r'GATC_y
Cuts at: 0 512 751 842 870 1206
Size: 512 239 91 28 336
Fragments arranged by size:
512 336 239 91 28
BstZ17I GTA'TAC
Cuts at: 0 581 1206
Size: 581 625
BtgI C'CryG_G
Cuts at: 0 18 128 185 220 1206
Size: 18 110 57 35 986
Fragments arranged by size:
986 110 57 35 18
BtsI GCAGTG_nn'
Cuts at: 0 459 818 1206
Size: 459 359 388
Fragments arranged by size:
459 388 359
EaeI y'GGCC_r
Cuts at: 0 15 276 293 1206
Size: 15 261 17 913
Fragments arranged by size:
913 261 17 15
EagI C'GGCC_G
Cuts at: 0 293 1206
Size: 293 913
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 242 1206
Size: 58 184 964
Fragments arranged by size:
964 184 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 783 1184 1206
Size: 783 401 22
Fragments arranged by size:
783 401 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 1092 1206
Size: 328 764 114
Fragments arranged by size:
764 328 114
EcoO109I rG'GnC_Cy
Cuts at: 0 1142 1206
Size: 1142 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 534 1043 1206
Size: 534 509 163
Fragments arranged by size:
534 509 163
HaeII r_GCGC'y
Cuts at: 0 324 404 933 1206
Size: 324 80 529 273
Fragments arranged by size:
529 324 273 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 1206
Size: 1156 32 18
Fragments arranged by size:
1156 32 18
HincII GTy'rAC
Cuts at: 0 1085 1206
Size: 1085 121
HpaI GTT'AAC
Cuts at: 0 1085 1206
Size: 1085 121
KasI G'GCGC_C
Cuts at: 0 320 400 1206
Size: 320 80 806
Fragments arranged by size:
806 320 80
MslI CAynn'nnrTG
Cuts at: 0 673 956 1206
Size: 673 283 250
Fragments arranged by size:
673 283 250
MspA1I CmG'CkG
Cuts at: 0 130 153 187 222 591 1206
Size: 130 23 34 35 369 615
Fragments arranged by size:
615 369 130 35 34 23
NaeI GCC'GGC
Cuts at: 0 116 276 297 372 1206
Size: 116 160 21 75 834
Fragments arranged by size:
834 160 116 75 21
NarI GG'CG_CC
Cuts at: 0 321 401 1206
Size: 321 80 805
Fragments arranged by size:
805 321 80
NgoMIV G'CCGG_C
Cuts at: 0 114 274 295 370 1206
Size: 114 160 21 75 836
Fragments arranged by size:
836 160 114 75 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 285 1206
Size: 285 921
PfoI T'CCnGG_A
Cuts at: 0 326 625 1206
Size: 326 299 581
Fragments arranged by size:
581 326 299
PstI C_TGCA'G
Cuts at: 0 269 912 1206
Size: 269 643 294
Fragments arranged by size:
643 294 269
PvuII CAG'CTG
Cuts at: 0 591 1206
Size: 591 615
SacII CC_GC'GG
Cuts at: 0 131 188 223 1206
Size: 131 57 35 983
Fragments arranged by size:
983 131 57 35
SfcI C'TryA_G
Cuts at: 0 265 908 1206
Size: 265 643 298
Fragments arranged by size:
643 298 265
SfoI GGC'GCC
Cuts at: 0 322 402 1206
Size: 322 80 804
Fragments arranged by size:
804 322 80
SmaI CCC'GGG
Cuts at: 0 357 1206
Size: 357 849
StyI C'CwwG_G
Cuts at: 0 747 1093 1206
Size: 747 346 113
Fragments arranged by size:
747 346 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1088 1206
Size: 1088 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 862 894 1206
Size: 862 32 312
Fragments arranged by size:
862 312 32
XmaI C'CCGG_G
Cuts at: 0 355 1206
Size: 355 851
Enzymes that do cut:
AccI AcuI AfeI ApoI AvaI AvrII BamHI BanI
BbeI BbsI BglI BglII Bme1580I BmrI BplI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI
BstZ17I BtgI BtsI EaeI EagI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HpaI KasI MslI
MspA1I NaeI NarI NgoMIV PflMI PfoI PstI PvuII
SacII SfcI SfoI SmaI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BanII BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsiHKAI BsiWI BsmI BspEI BsrDI BsrGI BssSI
BstBI BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EcoICRI EcoRI EcoRV FseI FspI FspAI
HindIII KpnI MfeI MluI MmeI MscI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmnI ZraI