Restriction map for
pFN21AE3988
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I Cac8I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 406 GTCTAC GT^[AC][GT]AC
AcsI 772 GAATTC [GA]^AATT[CT]
AfaI 123 GTAC GT^AC
AlwI 236 GATCC GGATC(4/5)
ApoI 772 GAATTC [GA]^AATT[CT]
BanII 724 GGGCTC G[GA]GC[CT]^C
BbrPI 154 CACGTG CAC^GTG
BbsI 91 GAAGAC GAAGAC(2/6)
BclI 854 TGATCA T^GATCA
BcnI 135 CCGGG CC^[GC]GG
BglII 596 AGATCT A^GATCT
BmyI 724 GGGCTC G[AGT]GC[ACT]^C
BpmI 40 CTGGAG CTGGAG(16/14)
BpuAI 91 GAAGAC GAAGAC(2/6)
BsaAI 154 CACGTG [CT]AC^GT[GA]
BsaBI 165 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 680 GTGCAG GTGCAG(16/14)
Bsp1286I 724 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 122 TGTACA T^GTACA
BspHI 768 TCATGA T^CATGA
BspMI 104 ACCTGC ACCTGC(4/8)
BsrDI 192 CATTGC GCAATG(2/0)
BsrGI 122 TGTACA T^GTACA
BstEII 249 GGTGACC G^GT[ACGT]ACC
BstPI 249 GGTGACC G^GT[ACGT]ACC
BstXI 149 CCATGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 596 AGATCT [GA]^GATC[CT]
DraII 44 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoNI 105 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 44 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 249 GGTGACC G^GT[ACGT]ACC
EcoRI 772 GAATTC G^AATTC
EcoT14I 527 CCATGG C^C[AT][AT]GG
EcoT22I 443 ATGCAT ATGCA^T
FbaI 854 TGATCA T^GATCA
HaeII 30 AGCGCC [GA]GCGC^[CT]
HindIII 79 AAGCTT A^AGCTT
KspI 5 CCGCGG CCGC^GG
MaeII 155 ACGT A^CGT
MamI 165 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 596 AGATCT [GA]^GATC[CT]
MseI 962 TTAA T^TAA
MslI 150 CATGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 5 CCGCGG C[AC]G^C[GT]G
NciI 135 CCGGG CC^[GC]GG
NcoI 527 CCATGG C^CATGG
NsiI 443 ATGCAT ATGCA^T
PmaCI 154 CACGTG CAC^GTG
PmlI 154 CACGTG CAC^GTG
RcaI 768 TCATGA T^CATGA
RsaI 123 GTAC GT^AC
SacII 5 CCGCGG CCGC^GG
SgrAI 32 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 122 TGTACA T^GTACA
StyI 527 CCATGG C^C[AT][AT]GG
Tru9I 962 TTAA T^TAA
Tsp45I 250 GTGAC ^GT[GC]AC
XhoII 596 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 6 CGCG CG^CG
20 CGCG
BfaI 518 CTAG C^TAG
973 CTAG
BsmAI 219 GAGAC GTCTC(1/5)
584 GAGAC
BsmFI 10 GGGAC GGGAC(10/14)
274 GTCCC
BsrFI 33 GCCGGT [GA]^CCGG[CT]
644 GCCGGT
BsrI 302 ACTGG ACTGG(1/-1)
347 CCAGT
BstUI 6 CGCG CG^CG
20 CGCG
Cfr10I 33 GCCGGT [GA]^CCGG[CT]
644 GCCGGT
Cfr13I 45 GGCCC G^G[ACGT]CC
138 GGGCC
DsaI 5 CCGCGG C^C[GA][CT]GG
527 CCATGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
231 TGGCCG
EarI 358 CTCTTC CTCTTC(1/4)
846 GAAGAG
FauI 51 CCCGC CCCGC(4/6)
7 GCGGG
HphI 249 GGTGA GGTGA(8/7)
939 GGTGA
Ksp632I 358 CTCTTC CTCTTC(1/4)
846 GAAGAG
MaeI 518 CTAG C^TAG
973 CTAG
MvnI 6 CGCG CG^CG
20 CGCG
PstI 86 CTGCAG CTGCA^G
106 CTGCAG
Sau96I 45 GGCCC G^G[ACGT]CC
138 GGGCC
SfaNI 129 GATGC GCATC(5/9)
779 GCATC
Tsp509I 296 AATT ^AATT
773 AATT
TspRI 214 CAGTG CA[GC]TG[ACGT][ACGT]^
636 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstF5I BstNI CfoI
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI SfcI
TfiI TseI