################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 340 GACGTC GACGT^C AccII 79 CGCG CG^CG AcyI 340 GACGTC G[GA]^CG[CT]C ApaI 3 GGGCCC GGGCC^C AseI 784 ATTAAT AT^TAAT AsnI 784 ATTAAT AT^TAAT AvaII 886 GGACC G^G[AT]CC BalI 90 TGGCCA TGG^CCA BamHI 695 GGATCC G^GATCC BanII 3 GGGCCC G[GA]GC[CT]^C BbsI 601 GAAGAC GAAGAC(2/6) BglI 5 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 3 GGGCCC G[AGT]GC[ACT]^C BpuAI 601 GAAGAC GAAGAC(2/6) BsaAI 292 TACGTG [CT]AC^GT[GA] BsaHI 340 GACGTC G[GA]^CG[CT]C BsaWI 451 ACCGGA [AT]^CCGG[AT] BsmI 954 GAATGC GAATGC(1/-1) Bsp1286I 3 GGGCCC G[AGT]GC[ACT]^C BspDI 252 ATCGAT AT^CGAT BspMI 123 GCAGGT ACCTGC(4/8) Bst1107I 67 GTATAC GTA^TAC BstEII 447 GGTGACC G^GT[ACGT]ACC BstPI 447 GGTGACC G^GT[ACGT]ACC BstUI 79 CGCG CG^CG BstZ17I 67 GTATAC GTA^TAC CfoI 111 GCGC GCG^C ClaI 252 ATCGAT AT^CGAT DdeI 48 CTCAG C^T[ACGT]AG EaeI 90 TGGCCA [CT]^GGCC[GA] EcoO65I 447 GGTGACC G^GT[ACGT]ACC EcoT14I 118 CCTTGG C^C[AT][AT]GG EcoT22I 956 ATGCAT ATGCA^T HaeII 110 GGCGCT [GA]GCGC^[CT] HapII 452 CCGG C^CGG HhaI 111 GCGC GCG^C Hin1I 340 GACGTC G[GA]^CG[CT]C HinP1I 111 GCGC G^CGC HpaII 452 CCGG C^CGG MluNI 90 TGGCCA TGG^CCA MscI 90 TGGCCA TGG^CCA MslI 679 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 452 CCGG C^CGG MvnI 79 CGCG CG^CG NsiI 956 ATGCAT ATGCA^T PshBI 784 ATTAAT AT^TAAT PstI 104 CTGCAG CTGCA^G ScaI 287 AGTACT AGT^ACT SfaNI 97 GATGC GCATC(5/9) StyI 118 CCTTGG C^C[AT][AT]GG TaqI 253 TCGA T^CGA TfiI 655 GATTC G^A[AT]TC Tsp509I 429 AATT ^AATT TthHB8I 253 TCGA T^CGA XbaI 813 TCTAGA T^CTAGA XcmI 433 CCACCCTTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 67 GTATAC GT^[AC][GT]AC 751 GTCTAC AciI 78 CCGC CCGC(-3/-1) 531 CCGC AflIII 279 ACGTGT A^C[GA][CT]GT 293 ACGTGT BfaI 247 CTAG C^TAG 814 CTAG BsiYI 33 CCTTCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 437 CCTTTTGTTGG BslI 33 CCTTCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 437 CCTTTTGTTGG BsmFI 869 GTCCC GGGAC(10/14) 971 GTCCC BstYI 44 GGATCT [GA]^GATC[CT] 695 GGATCC DraII 3 GGGCCCT [GA]G^G[ACGT]CC[CT] 663 AGGGCCT EarI 740 CTCTTC CTCTTC(1/4) 949 GAAGAG EcoO109I 3 GGGCCCT [GA]G^G[ACGT]CC[CT] 663 AGGGCCT Ksp632I 740 CTCTTC CTCTTC(1/4) 949 GAAGAG MaeI 247 CTAG C^TAG 814 CTAG MflI 44 GGATCT [GA]^GATC[CT] 695 GGATCC MseI 467 TTAA T^TAA 785 TTAA NlaIII 633 CATG CATG^ 959 CATG PleI 268 GACTC GAGTC(4/5) 749 GAGTC SfcI 104 CTGCAG C^T[GA][CT]AG 914 CTGTAG SmlI 270 CTCAAG C^T[CT][GA]AG 365 CTCAAG Tru9I 467 TTAA T^TAA 785 TTAA XhoII 44 GGATCT [GA]^GATC[CT] 695 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AfaI AluI AlwI BbvI BpmI BsaJI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TseI Tsp45I TspRI