(Circular) (Six-base) MAPSORT of: pFN21AE6387.seq Check: 9717 from: 1 to: 5689 pFN21AE6387 5689 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2349 3499 278 Size: 53 83 186 936 813 1150 2468 Fragments arranged by size: 2468 1150 936 813 186 83 53 AccI GT'mk_AC Cuts at: 1937 2073 2757 3032 1937 Size: 136 684 275 4594 Fragments arranged by size: 4594 684 275 136 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2492 2946 3752 5136 5404 1632 Size: 860 454 806 1384 268 1917 Fragments arranged by size: 1917 1384 860 806 454 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 2284 2298 4588 5565 1203 Size: 1081 14 2290 977 1327 Fragments arranged by size: 2290 1327 1081 977 14 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2224 1184 Size: 1040 4649 AlwNI CAG_nnn'CTG Cuts at: 3432 5004 3432 Size: 1572 4117 ApaI G_GGCC'C Cuts at: 2012 2012 Size: 5689 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 2790 4243 160 Size: 872 1758 1453 1606 Fragments arranged by size: 1758 1606 1453 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 3014 1870 Size: 73 1071 4545 Fragments arranged by size: 4545 1071 73 BamHI G'GATC_C Cuts at: 2700 3019 3386 2700 Size: 319 367 5003 Fragments arranged by size: 5003 367 319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 3008 1391 Size: 621 996 4072 Fragments arranged by size: 4072 996 621 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2613 928 Size: 971 714 4004 Fragments arranged by size: 4004 971 714 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BfrBI ATG'CAT Cuts at: 2963 2963 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2016 4300 136 Size: 107 122 71 631 605 344 2284 1525 Fragments arranged by size: 2284 1525 631 605 344 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2012 3750 4906 1790 Size: 222 1738 1156 2573 Fragments arranged by size: 2573 1738 1156 222 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2406 3420 4378 Size: 416 53 83 399 24 989 1014 958 Cuts at: 4378 442 Size: 1753 Fragments arranged by size: 1753 1014 989 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2189 2189 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2609 2685 2772 4349 1239 Size: 823 547 76 87 1577 2579 Fragments arranged by size: 2579 1577 823 547 87 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2260 2355 3814 4679 4977 2260 Size: 95 1459 865 298 2972 Fragments arranged by size: 2972 1459 865 298 95 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2299 3480 493 Size: 711 1095 1181 2702 Fragments arranged by size: 2702 1181 1095 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2346 3496 3878 4513 Size: 53 83 186 936 813 1150 382 635 Cuts at: 4513 275 Size: 1451 Fragments arranged by size: 1451 1150 936 813 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2456 4122 4520 4794 4941 5352 2456 Size: 1666 398 274 147 411 2793 Fragments arranged by size: 2793 1666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2959 2989 1178 Size: 30 165 30 1556 30 3878 Fragments arranged by size: 3878 1556 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5689 BseYI C'CCAG_C Cuts at: 2044 2721 2949 4892 5388 2044 Size: 677 228 1943 496 2345 Fragments arranged by size: 2345 1943 677 496 228 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3750 3835 4906 3750 Size: 85 1071 4533 Fragments arranged by size: 4533 1071 85 BsmI GAATG_Cn' Cuts at: 2965 3200 3293 2965 Size: 235 93 5361 Fragments arranged by size: 5361 235 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 3008 3750 3835 4906 1391 Size: 399 222 996 742 85 1071 2174 Fragments arranged by size: 2174 1071 996 742 399 222 85 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2119 3044 844 Size: 1275 925 3489 Fragments arranged by size: 3489 1275 925 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 2452 1184 Size: 1268 4421 BstYI r'GATC_y Cuts at: 1972 2049 2700 3019 3386 3771 3788 5240 5326 Size: 77 651 319 367 385 17 1452 86 Cuts at: 5326 5338 5684 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 651 385 367 346 319 86 77 17 12 BstZ17I GTA'TAC Cuts at: 2074 2074 Size: 5689 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2206 3205 3999 4019 2206 Size: 999 794 20 3876 Fragments arranged by size: 3876 999 794 20 ClaI AT'CG_AT Cuts at: 1554 2258 1554 Size: 704 4985 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 3136 4025 Size: 54 1425 50 275 18 265 1041 889 Cuts at: 4025 5654 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1041 889 275 265 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2751 2949 3619 1353 Size: 1398 198 670 3423 Fragments arranged by size: 3423 1398 670 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2492 2609 2685 2772 2946 3752 Size: 393 430 430 117 76 87 174 806 Cuts at: 3752 4349 5136 5404 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 806 787 597 430 430 393 268 174 117 87 76 EcoO109I rG'GnC_Cy Cuts at: 2009 2669 2009 Size: 660 5029 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2119 4836 1678 Size: 441 2717 2531 Fragments arranged by size: 2717 2531 441 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2959 2991 4330 4362 4404 Size: 32 135 32 1387 32 1339 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1387 1339 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4803 4987 1305 Size: 3498 184 2007 Fragments arranged by size: 3498 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2097 10 Size: 54 1425 343 265 3602 Fragments arranged by size: 3602 1425 343 265 54 MslI CAynn'nnrTG Cuts at: 518 1166 2688 3647 4006 4165 518 Size: 648 1522 959 359 159 2042 Fragments arranged by size: 2042 1522 959 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3105 3782 4930 5175 1606 Size: 1499 677 1148 245 2120 Fragments arranged by size: 2120 1499 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4198 Fragments arranged by size: 4198 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NsiI A_TGCA'T Cuts at: 2965 2965 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 2979 1348 Size: 1631 4058 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PfoI T'CCnGG_A Cuts at: 2749 2749 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2008 2008 Size: 5689 PstI C_TGCA'G Cuts at: 830 2113 3041 830 Size: 1283 928 3478 Fragments arranged by size: 3478 1283 928 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 2294 3937 5649 2294 Size: 1643 1712 2334 Fragments arranged by size: 2334 1712 1643 SfcI C'TryA_G Cuts at: 826 1045 2109 2919 3037 4172 4853 5044 826 Size: 219 1064 810 118 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 810 681 219 191 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2275 2370 3793 4694 4956 820 Size: 197 926 332 95 1423 901 262 1553 Fragments arranged by size: 1553 1423 926 901 332 262 197 95 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2004 2123 3128 513 Size: 552 939 119 1005 3074 Fragments arranged by size: 3074 1005 939 552 119 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2440 3716 3733 3886 4071 4516 325 Size: 1255 860 1276 17 153 185 445 1498 Fragments arranged by size: 1498 1276 1255 860 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 3935 5647 Size: 274 80 33 51 1443 315 1643 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1643 1443 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5649 5681 5649 Size: 32 5657 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 2818 3025 2818 Size: 207 5482 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2445 2445 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2347 3497 276 Size: 53 83 186 936 813 1150 2468 Fragments arranged by size: 2468 1150 936 813 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI