(Circular) (Six-base) MAPSORT of: pFN21AE2202.seq Check: 1734 from: 1 to: 7534 pFN21AE2202 7534 bp With 182 enzymes: SgfI * September 21, 2016 11:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5344 278 Size: 53 83 186 936 3808 2468 Fragments arranged by size: 3808 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2059 4450 4578 4877 1937 Size: 122 2391 128 299 4594 Fragments arranged by size: 4594 2391 299 128 122 Acc65I G'GTAC_C Cuts at: 4855 4855 Size: 7534 AclI AA'CG_TT Cuts at: 1563 5661 6034 1563 Size: 4098 373 3063 Fragments arranged by size: 4098 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3315 3555 3621 3816 4023 4746 5597 6981 Size: 1683 240 66 195 207 723 851 1384 Cuts at: 6981 7249 1632 Size: 268 1917 Fragments arranged by size: 1917 1683 1384 851 723 268 240 207 195 66 AfeI AGC'GCT Cuts at: 3500 3500 Size: 7534 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7337 AflIII A'CryG_T Cuts at: 1203 3989 4232 6433 7410 1203 Size: 2786 243 2201 977 1327 Fragments arranged by size: 2786 2201 1327 977 243 AgeI A'CCGG_T Cuts at: 6365 6365 Size: 7534 AhdI GACnn_n'nnGTC Cuts at: 6263 6263 Size: 7534 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7534 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7534 AlwNI CAG_nnn'CTG Cuts at: 5277 6849 5277 Size: 1572 5962 ApaLI G'TGCA_C Cuts at: 5591 6747 5591 Size: 1156 6378 ApoI r'AATT_y Cuts at: 1493 2164 2361 2456 2566 2763 2909 3275 3362 Size: 671 197 95 110 197 146 366 87 Cuts at: 3362 3373 3570 3828 4843 5069 5221 7330 1493 Size: 11 197 258 1015 226 152 2109 1697 Fragments arranged by size: 2109 1697 1015 671 366 258 226 197 197 197 152 146 110 95 87 11 AseI AT'TA_AT Cuts at: 160 1032 6088 160 Size: 872 5056 1606 Fragments arranged by size: 5056 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7534 AvaI C'yCGr_G Cuts at: 1870 1943 4859 1870 Size: 73 2916 4545 Fragments arranged by size: 4545 2916 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2493 2526 2493 Size: 33 7501 BamHI G'GATC_C Cuts at: 4864 5231 4864 Size: 367 7167 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4855 6310 618 Size: 325 217 627 3068 1455 1842 Fragments arranged by size: 3068 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4853 1391 Size: 3462 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3755 928 Size: 971 1856 4707 Fragments arranged by size: 4707 1856 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5725 5759 1903 Size: 34 3788 34 3678 Fragments arranged by size: 3788 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3142 4585 5426 6636 3142 Size: 1443 841 1210 4040 Fragments arranged by size: 4040 1443 1210 841 BclI T'GATC_A Cuts at: 1548 2431 1548 Size: 883 6651 BfrBI ATG'CAT Cuts at: 3661 3661 Size: 7534 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6145 136 Size: 107 122 71 631 605 4473 1525 Fragments arranged by size: 4473 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2948 3191 3908 7529 2948 Size: 243 717 3621 2953 Fragments arranged by size: 3621 2953 717 243 BlpI GC'TnA_GC Cuts at: 4951 4951 Size: 7534 Bme1580I G_kGCm'C Cuts at: 1790 2074 2539 5595 6751 1790 Size: 284 465 3056 1156 2573 Fragments arranged by size: 3056 2573 1156 465 284 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3104 4620 4749 Size: 416 53 83 399 24 1687 1516 129 Cuts at: 4749 5265 6223 442 Size: 516 958 1753 Fragments arranged by size: 1753 1687 1516 958 516 416 399 129 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7534 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3825 4044 4308 6194 1239 Size: 2586 219 264 1886 2579 Fragments arranged by size: 2586 2579 1886 264 219 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5659 6524 6822 5659 Size: 865 298 6371 Fragments arranged by size: 6371 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2985 6197 882 Size: 617 1486 3212 2219 Fragments arranged by size: 3212 2219 1486 617 BsaAI yAC'GTr Cuts at: 493 1204 2075 5325 493 Size: 711 871 3250 2702 Fragments arranged by size: 3250 2702 871 711 BsaBI GATnn'nnATC Cuts at: 4542 7528 4542 Size: 2986 4548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3580 5341 5723 6358 Size: 53 83 186 936 2047 1761 382 635 Cuts at: 6358 275 Size: 1451 Fragments arranged by size: 2047 1761 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2015 4791 5967 6365 6639 6786 7197 2015 Size: 2776 1176 398 274 147 411 2352 Fragments arranged by size: 2776 2352 1176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3798 3828 4287 4317 1178 Size: 30 165 30 2395 30 459 30 4395 Fragments arranged by size: 4395 2395 459 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3626 4275 1186 Size: 2440 649 4445 Fragments arranged by size: 4445 2440 649 BseYI C'CCAG_C Cuts at: 6737 7233 6737 Size: 496 7038 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3003 3933 1163 Size: 573 1267 930 4764 Fragments arranged by size: 4764 1267 930 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4984 5745 6773 1540 Size: 275 186 2983 761 1028 2301 Fragments arranged by size: 2983 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3017 5595 5680 6751 3017 Size: 2578 85 1071 3800 Fragments arranged by size: 3800 2578 1071 85 BsmI GAATG_Cn' Cuts at: 3226 5045 5138 3226 Size: 1819 93 5622 Fragments arranged by size: 5622 1819 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2539 3017 4853 5595 5680 6751 Size: 399 284 465 478 1836 742 85 1071 Cuts at: 6751 1391 Size: 2174 Fragments arranged by size: 2174 1836 1071 742 478 465 399 284 85 BspEI T'CCGG_A Cuts at: 4791 7197 4791 Size: 2406 5128 BspHI T'CATG_A Cuts at: 3820 5423 7153 3820 Size: 1603 1730 4201 Fragments arranged by size: 4201 1730 1603 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4889 844 Size: 4045 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5421 1394 Size: 429 3598 3507 Fragments arranged by size: 3598 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6029 6203 65 Size: 1183 4781 174 1396 Fragments arranged by size: 4781 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6178 6365 1700 Size: 110 4368 187 2869 Fragments arranged by size: 4368 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7534 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7534 BssSI C'ACGA_G Cuts at: 5594 6606 5594 Size: 1012 6522 BstAPI GCAn_nnn'nTGC Cuts at: 794 2235 794 Size: 1441 6093 BstBI TT'CG_AA Cuts at: 6338 6338 Size: 7534 BstEII G'GTnAC_C Cuts at: 1184 4694 1184 Size: 3510 4024 BstXI CCAn_nnnn'nTGG Cuts at: 3591 3591 Size: 7534 BstYI r'GATC_y Cuts at: 1972 2948 3191 3908 4398 4462 4519 4864 5231 Size: 976 243 717 490 64 57 345 367 Cuts at: 5231 5616 5633 7085 7171 7183 7529 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 976 717 490 385 367 346 345 243 86 64 57 17 12 BstZ17I GTA'TAC Cuts at: 4451 4579 4451 Size: 128 7406 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6043 Fragments arranged by size: 6043 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6795 Fragments arranged by size: 6795 683 56 BtsI GCAGTG_nn' Cuts at: 3015 5050 5844 5864 3015 Size: 2035 794 20 4685 Fragments arranged by size: 4685 2035 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7534 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7499 DraI TTT'AAA Cuts at: 3789 4838 3789 Size: 1049 6485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6541 809 Size: 507 5225 1802 Fragments arranged by size: 5225 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4660 4981 5870 Size: 54 1425 50 275 18 2830 321 889 Cuts at: 5870 7499 8 Size: 1629 43 Fragments arranged by size: 2830 1629 1425 889 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4981 1537 Size: 275 3169 4090 Fragments arranged by size: 4090 3169 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2679 3387 3396 5464 1353 Size: 1326 708 9 2068 3423 Fragments arranged by size: 3423 2068 1326 708 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6121 6495 6641 387 Size: 1064 138 4532 374 146 1280 Fragments arranged by size: 4532 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3315 3555 3621 3816 3825 4023 4044 Size: 393 1683 240 66 195 9 198 21 Cuts at: 4044 4308 4746 5597 6194 6981 7249 1239 Size: 264 438 851 597 787 268 1524 Fragments arranged by size: 1683 1524 851 787 597 438 393 268 264 240 198 195 66 21 9 EcoO109I rG'GnC_Cy Cuts at: 4489 4489 Size: 7534 EcoRI G'AATT_C Cuts at: 4843 5221 4843 Size: 378 7156 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3177 3320 3517 4077 4447 3177 Size: 143 197 560 370 6264 Fragments arranged by size: 6264 560 370 197 143 FspI TGC'GCA Cuts at: 6040 6040 Size: 7534 HaeII r_GCGC'y Cuts at: 1678 3502 6681 1678 Size: 1824 3179 2531 Fragments arranged by size: 3179 2531 1824 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2681 2713 4319 4351 6175 Size: 32 135 32 1109 32 1606 32 1824 Cuts at: 6175 6207 6249 6281 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1824 1606 1109 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2060 4375 4687 4878 5124 669 Size: 988 281 122 2315 312 191 246 3079 Fragments arranged by size: 3079 2315 988 312 281 246 191 122 HindIII A'AGCT_T Cuts at: 748 748 Size: 7534 HpaI GTT'AAC Cuts at: 4687 5124 4687 Size: 437 7097 KpnI G_GTAC'C Cuts at: 4859 4859 Size: 7534 MfeI C'AATT_G Cuts at: 5133 5133 Size: 7534 MluI A'CGCG_T Cuts at: 7410 7410 Size: 7534 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2907 4224 6648 6832 1305 Size: 1602 1317 2424 184 2007 Fragments arranged by size: 2424 2007 1602 1317 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4662 10 Size: 54 1425 343 2830 2882 Fragments arranged by size: 2882 2830 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2439 5492 5851 6010 518 Size: 648 1273 3053 359 159 2042 Fragments arranged by size: 3053 2042 1273 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3293 4950 5627 6775 7020 1606 Size: 1687 1657 677 1148 245 2120 Fragments arranged by size: 2120 1687 1657 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6043 Fragments arranged by size: 6043 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7534 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7534 NotI GC'GGCC_GC Cuts at: 4981 4981 Size: 7534 NsiI A_TGCA'T Cuts at: 3663 3663 Size: 7534 NspI r_CATG'y Cuts at: 1132 1229 2852 3993 4658 4892 6437 1132 Size: 97 1623 1141 665 234 1545 2229 Fragments arranged by size: 2229 1623 1545 1141 665 234 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7534 PciI A'CATG_T Cuts at: 3989 6433 3989 Size: 2444 5090 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7534 PmeI GTTT'AAAC Cuts at: 4838 4838 Size: 7534 PmlI CAC'GTG Cuts at: 2075 2075 Size: 7534 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5603 5635 7116 7148 5603 Size: 32 1481 32 5989 Fragments arranged by size: 5989 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4489 4489 Size: 7534 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7534 PsiI TTA'TAA Cuts at: 2915 4776 5104 2915 Size: 1861 328 5345 Fragments arranged by size: 5345 1861 328 PstI C_TGCA'G Cuts at: 830 4886 830 Size: 4056 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7534 PvuII CAG'CTG Cuts at: 3293 3293 Size: 7534 SalI G'TCGA_C Cuts at: 1936 2058 4876 1936 Size: 122 2818 4594 Fragments arranged by size: 4594 2818 122 SapI GCTCTTCn'nnn_ Cuts at: 3387 3387 Size: 7534 SbfI CC_TGCA'GG Cuts at: 4886 4886 Size: 7534 ScaI AGT'ACT Cuts at: 3144 4253 4317 4738 5782 7494 3144 Size: 1109 64 421 1044 1712 3184 Fragments arranged by size: 3184 1712 1109 1044 421 64 SfcI C'TryA_G Cuts at: 826 1045 2041 2294 4882 6017 6698 6889 826 Size: 219 996 253 2588 1135 681 191 1471 Fragments arranged by size: 2588 1471 1135 996 681 253 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7534 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7534 SmaI CCC'GGG Cuts at: 1872 4861 1872 Size: 2989 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5638 6539 6801 820 Size: 197 926 3695 901 262 1553 Fragments arranged by size: 3695 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5325 493 Size: 4832 2702 SpeI A'CTAG_T Cuts at: 152 4186 152 Size: 4034 3500 SphI G_CATG'C Cuts at: 1132 2852 4892 1132 Size: 1720 2040 3774 Fragments arranged by size: 3774 2040 1720 SspI AAT'ATT Cuts at: 5 52 3715 4363 5458 5 Size: 47 3663 648 1095 2081 Fragments arranged by size: 3663 2081 1095 648 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7534 StyI C'CwwG_G Cuts at: 513 1065 2004 4973 513 Size: 552 939 2969 3074 Fragments arranged by size: 3074 2969 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3123 3586 5561 5578 5731 5916 6361 Size: 1255 1543 463 1975 17 153 185 445 Cuts at: 6361 325 Size: 1498 Fragments arranged by size: 1975 1543 1498 1255 463 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2159 2591 3142 Size: 274 80 33 51 1443 182 432 551 Cuts at: 3142 3775 4251 4315 4706 4736 5780 7492 96 Size: 633 476 64 391 30 1044 1712 138 Fragments arranged by size: 1712 1443 1044 633 551 476 432 391 274 182 138 80 64 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4287 4319 7494 7526 4287 Size: 32 3175 32 4295 Fragments arranged by size: 4295 3175 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7534 XbaI T'CTAG_A Cuts at: 3236 3905 4870 3236 Size: 669 965 5900 Fragments arranged by size: 5900 965 669 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3591 3591 Size: 7534 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7534 XmaI C'CCGG_G Cuts at: 1870 4859 1870 Size: 2989 4545 XmnI GAAnn'nnTTC Cuts at: 3571 4806 5663 3571 Size: 1235 857 5442 Fragments arranged by size: 5442 1235 857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5342 276 Size: 53 83 186 936 3808 2468 Fragments arranged by size: 3808 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI