(Circular) (Six-base) MAPSORT of: pFN21AE3675.seq Check: 5732 from: 1 to: 5803 pFN21AE3675 5803 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3047 3613 278 Size: 53 83 186 936 1511 566 2468 Fragments arranged by size: 2468 1511 936 566 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2396 2632 3866 5250 5518 1632 Size: 506 258 236 1234 1384 268 1917 Fragments arranged by size: 1917 1384 1234 506 268 258 236 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 4532 4532 Size: 5803 AleI CACnn'nnGTG Cuts at: 1166 2954 1166 Size: 1788 4015 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2379 1184 Size: 1195 4608 AlwNI CAG_nnn'CTG Cuts at: 3071 3546 5118 3071 Size: 475 1572 3756 Fragments arranged by size: 3756 1572 475 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 2372 3029 3061 3112 3338 3490 5599 1493 Size: 879 657 32 51 226 152 2109 1697 Fragments arranged by size: 2109 1697 879 657 226 152 51 32 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 3128 1870 Size: 73 1185 4545 Fragments arranged by size: 4545 1185 73 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 3124 4579 618 Size: 325 217 627 255 1082 1455 1842 Fragments arranged by size: 1842 1455 1082 627 325 255 217 BanII G_rGCy'C Cuts at: 1391 3122 1391 Size: 1731 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2821 928 Size: 971 922 3910 Fragments arranged by size: 3910 971 922 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4414 136 Size: 107 122 71 631 605 2742 1525 Fragments arranged by size: 2742 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5798 5798 Size: 5803 BlpI GC'TnA_GC Cuts at: 3220 3220 Size: 5803 Bme1580I G_kGCm'C Cuts at: 1790 2045 3864 5020 1790 Size: 255 1819 1156 2573 Fragments arranged by size: 2573 1819 1156 255 BmgBI CAC'GTC Cuts at: 1318 2238 1318 Size: 920 4883 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3534 4492 442 Size: 416 53 83 399 24 2117 958 1753 Fragments arranged by size: 2117 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2845 4463 1239 Size: 1606 1618 2579 Fragments arranged by size: 2579 1618 1606 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3000 3928 4793 5091 3000 Size: 928 865 298 3712 Fragments arranged by size: 3712 928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4466 882 Size: 617 2967 2219 Fragments arranged by size: 2967 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3594 493 Size: 711 2390 2702 Fragments arranged by size: 2702 2390 711 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2289 3044 3610 3992 Size: 53 83 186 936 756 755 566 382 Cuts at: 3992 4627 275 Size: 635 1451 Fragments arranged by size: 1451 936 756 755 635 566 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4236 4634 4908 5055 5466 4236 Size: 398 274 147 411 4573 Fragments arranged by size: 4573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 2129 2159 2177 Size: 30 165 30 606 30 90 30 18 Cuts at: 2177 2207 1178 Size: 30 4774 Fragments arranged by size: 4774 606 165 90 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2432 1186 Size: 1246 4557 BseYI C'CCAG_C Cuts at: 2208 5006 5502 2208 Size: 2798 496 2509 Fragments arranged by size: 2798 2509 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2747 2780 1163 Size: 573 1011 33 4186 Fragments arranged by size: 4186 1011 573 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3864 3949 5020 3864 Size: 85 1071 4647 Fragments arranged by size: 4647 1071 85 BsmI GAATG_Cn' Cuts at: 3314 3407 3314 Size: 93 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 3122 3864 3949 5020 1391 Size: 399 255 1077 742 85 1071 2174 Fragments arranged by size: 2174 1077 1071 742 399 255 85 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 2368 3692 5422 2368 Size: 1324 1730 2749 Fragments arranged by size: 2749 1730 1324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2482 3158 844 Size: 1638 676 3489 Fragments arranged by size: 3489 1638 676 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2262 4298 4472 65 Size: 1183 1014 2036 174 1396 Fragments arranged by size: 2036 1396 1183 1014 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4447 4634 1700 Size: 110 2637 187 2869 Fragments arranged by size: 2869 2637 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 3863 4875 3863 Size: 1012 4791 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5803 BstBI TT'CG_AA Cuts at: 4607 4607 Size: 5803 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5803 BstXI CCAn_nnnn'nTGG Cuts at: 2755 2755 Size: 5803 BstYI r'GATC_y Cuts at: 1972 3133 3500 3885 3902 5354 5440 5452 5798 Size: 1161 367 385 17 1452 86 12 346 Cuts at: 5798 1972 Size: 1977 Fragments arranged by size: 1977 1452 1161 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5251 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2005 487 Size: 683 56 779 4285 Fragments arranged by size: 4285 779 683 56 BtsI GCAGTG_nn' Cuts at: 2877 2929 3319 4113 4133 2877 Size: 52 390 794 20 4547 Fragments arranged by size: 4547 794 390 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 2379 3107 2379 Size: 728 5075 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3250 4139 5768 Size: 54 1425 50 275 18 1420 889 1629 Cuts at: 5768 8 Size: 43 Fragments arranged by size: 1629 1425 1420 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2855 3733 1353 Size: 1502 878 3423 Fragments arranged by size: 3423 1502 878 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2261 4390 4764 4910 387 Size: 1064 138 672 2129 374 146 1280 Fragments arranged by size: 2129 1280 1064 672 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2396 2632 2845 3866 4463 5250 Size: 393 506 258 236 213 1021 597 787 Cuts at: 5250 5518 1239 Size: 268 1524 Fragments arranged by size: 1524 1021 787 597 506 393 268 258 236 213 EcoNI CCTnn'n_nnAGG Cuts at: 2414 2414 Size: 5803 EcoRI G'AATT_C Cuts at: 3029 3112 3490 3029 Size: 83 378 5342 Fragments arranged by size: 5342 378 83 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2245 2606 2245 Size: 361 5442 FspI TGC'GCA Cuts at: 4309 4309 Size: 5803 HaeII r_GCGC'y Cuts at: 1678 4950 1678 Size: 3272 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4444 4476 4518 4550 1373 Size: 32 135 32 2872 32 42 32 2626 Fragments arranged by size: 2872 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3147 3393 669 Size: 988 281 1209 246 3079 Fragments arranged by size: 3079 1209 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4917 5101 1305 Size: 3612 184 2007 Fragments arranged by size: 3612 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3981 Fragments arranged by size: 3981 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2954 3761 4120 4279 518 Size: 648 1788 807 359 159 2042 Fragments arranged by size: 2042 1788 807 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2794 3219 3896 5044 5289 1606 Size: 1188 425 677 1148 245 2120 Fragments arranged by size: 2120 1188 1148 677 425 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5251 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 2136 2356 2700 2890 3161 4706 1132 Size: 97 907 220 344 190 271 1545 2229 Fragments arranged by size: 2229 1545 907 344 271 220 190 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5803 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5803 PfoI T'CCnGG_A Cuts at: 2181 2181 Size: 5803 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2832 2864 3872 3904 5385 5417 2832 Size: 32 1008 32 1481 32 3218 Fragments arranged by size: 3218 1481 1008 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2073 1613 Size: 460 5343 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PstI C_TGCA'G Cuts at: 830 2705 3155 830 Size: 1875 450 3478 Fragments arranged by size: 3478 1875 450 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 PvuII CAG'CTG Cuts at: 2794 2794 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SapI GCTCTTCn'nnn_ Cuts at: 2855 2855 Size: 5803 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SfcI C'TryA_G Cuts at: 826 1045 2701 3151 4286 4967 5158 826 Size: 219 1656 450 1135 681 191 1471 Fragments arranged by size: 1656 1471 1135 681 450 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 3130 1872 Size: 1258 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3015 3907 4808 5070 820 Size: 197 926 1072 892 901 262 1553 Fragments arranged by size: 1553 1072 926 901 892 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 3161 1132 Size: 2029 3774 SspI AAT'ATT Cuts at: 5 52 2684 3727 5 Size: 47 2632 1043 2081 Fragments arranged by size: 2632 2081 1043 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5803 StyI C'CwwG_G Cuts at: 513 1065 3242 513 Size: 552 2177 3074 Fragments arranged by size: 3074 2177 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3830 3847 4000 4185 4630 325 Size: 1255 2250 17 153 185 445 1498 Fragments arranged by size: 2250 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 2781 4049 Size: 274 80 33 51 1443 186 618 1268 Cuts at: 4049 5761 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1268 618 274 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5763 5795 5763 Size: 32 5771 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 2499 2035 Size: 464 5339 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 3128 1870 Size: 1258 4545 XmnI GAAnn'nnTTC Cuts at: 2586 3932 2586 Size: 1346 4457 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3045 3611 276 Size: 53 83 186 936 1511 566 2468 Fragments arranged by size: 2468 1511 936 566 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI