################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BamHI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspMI BsrBI BsrDI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluNI MroI MscI MunI NcoI NdeI NheI NotI NsiI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TspRI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 878 GGTACC G^GTACC AcsI 1218 GAATTT [GA]^AATT[CT] ApaI 187 GGGCCC GGGCC^C ApoI 1218 GAATTT [GA]^AATT[CT] Asp718I 878 GGTACC G^GTACC AspI 1192 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 209 GGACC G^G[AT]CC BbeI 115 GGCGCC GGCGC^C BbrPI 613 CACGTG CAC^GTG BbsI 1116 GAAGAC GAAGAC(2/6) BbvCI 774 GCTGAGG CCTCAGC(-5/-2) BlpI 475 GCTGAGC GC^T[ACGT]AGC Bpu1102I 475 GCTGAGC GC^T[ACGT]AGC BpuAI 1116 GAAGAC GAAGAC(2/6) BsgI 66 CTGCAC GTGCAG(16/14) BsiEI 110 CGGCCG CG[GA][CT]^CG BsmBI 544 GAGACG CGTCTC(1/5) Bsp1407I 338 TGTACA T^GTACA BspLU11I 620 ACATGT A^CATGT BsrGI 338 TGTACA T^GTACA BssHII 606 GCGCGC G^CGCGC CelII 475 GCTGAGC GC^T[ACGT]AGC DraI 1083 TTTAAA TTT^AAA DraIII 69 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 110 CGGCCG C^GGCCG EclXI 110 CGGCCG C^GGCCG Eco52I 110 CGGCCG C^GGCCG EcoT14I 746 CCAAGG C^C[AT][AT]GG HincII 568 GTCAAC GT[CT]^[GA]AC HindII 568 GTCAAC GT[CT]^[GA]AC KasI 115 GGCGCC G^GCGCC KpnI 878 GGTACC GGTAC^C MluI 1253 ACGCGT A^CGCGT NarI 115 GGCGCC GG^CGCC PflFI 1192 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 153 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 613 CACGTG CAC^GTG PmlI 613 CACGTG CAC^GTG SacI 1045 GAGCTC GAGCT^C ScaI 20 AGTACT AGT^ACT SfoI 115 GGCGCC GGC^GCC SmlI 1089 CTTGAG C^T[CT][GA]AG SspBI 338 TGTACA T^GTACA StuI 362 AGGCCT AGG^CCT StyI 746 CCAAGG C^C[AT][AT]GG TfiI 1157 GAATC G^A[AT]TC Tth111I 1192 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 153 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 115 GGCGCC G[GA]^CG[CT]C 238 GACGCC AflIII 620 ACATGT A^C[GA][CT]GT 1253 ACGCGT AlwNI 226 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 484 CAGCAGCTG AspHI 1045 GAGCTC G[AT]GC[AT]^C 1069 GTGCTC AvaI 97 CCCGAG C^[CT]CG[GA]G 190 CCCGAG BcnI 299 CCCGG CC^[GC]GG 852 CCGGG BfaI 970 CTAG C^TAG 1181 CTAG BpmI 1141 CTCCAG CTGGAG(16/14) 1245 CTCCAG BsaHI 115 GGCGCC G[GA]^CG[CT]C 238 GACGCC BseRI 220 GAGGAG GAGGAG(10/8) 751 GAGGAG BsiHKAI 1045 GAGCTC G[AT]GC[AT]^C 1069 GTGCTC BsmAI 544 GAGAC GTCTC(1/5) 645 GAGAC BsmFI 1250 GGGAC GGGAC(10/14) 410 GTCCC BsoBI 97 CCCGAG C^[CT]CG[GA]G 190 CCCGAG BstAPI 68 GCACCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 225 GCAGCTGCTGC BstYI 860 AGATCC [GA]^GATC[CT] 1241 GGATCT DdeI 476 CTGAG C^T[ACGT]AG 775 CTGAG DraII 186 GGGGCCC [GA]G^G[ACGT]CC[CT] 420 AGGCCCC DsaI 144 CCACGG C^C[GA][CT]GG 345 CCACGG EarI 907 GAAGAG CTCTTC(1/4) 948 GAAGAG EcoO109I 186 GGGGCCC [GA]G^G[ACGT]CC[CT] 420 AGGCCCC Hin1I 115 GGCGCC G[GA]^CG[CT]C 238 GACGCC Ksp632I 907 GAAGAG CTCTTC(1/4) 948 GAAGAG MaeI 970 CTAG C^TAG 1181 CTAG MflI 860 AGATCC [GA]^GATC[CT] 1241 GGATCT MseI 1084 TTAA T^TAA 1130 TTAA NaeI 112 GCCGGC GCC^GGC 973 GCCGGC NciI 299 CCCGG CC^[GC]GG 852 CCGGG NgoMIV 112 GCCGGC G^CCGGC 973 GCCGGC NruI 216 TCGCGA TCG^CGA 1059 TCGCGA PstI 871 CTGCAG CTGCA^G 1074 CTGCAG SfaNI 538 GATGC GCATC(5/9) 368 GCATC SfcI 871 CTGCAG C^T[GA][CT]AG 1074 CTGCAG Tru9I 1084 TTAA T^TAA 1130 TTAA Tsp509I 1128 AATT ^AATT 1219 AATT XhoII 860 AGATCC [GA]^GATC[CT] 1241 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BanI BanII BbvI BmyI BsaAI BsaJI BsiYI BslI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII EaeI EcoNI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI TaqI TseI Tsp45I TthHB8I