(Circular) (Six-base) MAPSORT of: pFN21AE1142.seq Check: 3858 from: 1 to: 5965 pFN21AE1142 5965 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3308 1937 Size: 1371 4594 Acc65I G'GTAC_C Cuts at: 2883 3286 2883 Size: 403 5562 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 2756 2795 4028 5412 5680 1632 Size: 995 129 39 1233 1384 268 1917 Fragments arranged by size: 1917 1384 1233 995 268 129 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5768 AflIII A'CryG_T Cuts at: 1203 2625 3258 4864 5841 1203 Size: 1422 633 1606 977 1327 Fragments arranged by size: 1606 1422 1327 977 633 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 2295 1166 Size: 1129 4836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5965 AlwNI CAG_nnn'CTG Cuts at: 2236 2494 3708 5280 2236 Size: 258 1214 1572 2921 Fragments arranged by size: 2921 1572 1214 258 ApaI G_GGCC'C Cuts at: 2196 2196 Size: 5965 ApaLI G'TGCA_C Cuts at: 4022 5178 4022 Size: 1156 4809 ApoI r'AATT_y Cuts at: 1493 3223 3274 3500 3652 5761 1493 Size: 1730 51 226 152 2109 1697 Fragments arranged by size: 2109 1730 1697 226 152 51 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 2102 2195 3290 1870 Size: 73 159 93 1095 4545 Fragments arranged by size: 4545 1095 159 93 73 BamHI G'GATC_C Cuts at: 3295 3662 3295 Size: 367 5598 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 2689 2883 3286 4741 Size: 325 217 627 333 569 194 403 1455 Cuts at: 4741 618 Size: 1842 Fragments arranged by size: 1842 1455 627 569 403 333 325 217 194 BanII G_rGCy'C Cuts at: 1391 2057 2196 2568 3054 3284 1391 Size: 666 139 372 486 230 4072 Fragments arranged by size: 4072 666 486 372 230 139 BbeI G_GCGC'C Cuts at: 2124 2124 Size: 5965 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3128 928 Size: 971 1229 3765 Fragments arranged by size: 3765 1229 971 BbvCI CC'TCA_GC Cuts at: 2780 2780 Size: 5965 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4156 4190 1903 Size: 34 2219 34 3678 Fragments arranged by size: 3678 2219 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 5067 3857 Size: 1210 4755 BclI T'GATC_A Cuts at: 1548 1548 Size: 5965 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4576 136 Size: 107 122 71 631 605 2904 1525 Fragments arranged by size: 2904 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5960 5960 Size: 5965 BlpI GC'TnA_GC Cuts at: 2481 3382 2481 Size: 901 5064 Bme1580I G_kGCm'C Cuts at: 1790 2102 2196 4026 5182 1790 Size: 312 94 1830 1156 2573 Fragments arranged by size: 2573 1830 1156 312 94 BmgBI CAC'GTC Cuts at: 1318 2593 1318 Size: 1275 4690 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2281 3696 4654 Size: 416 53 83 399 24 864 1415 958 Cuts at: 4654 442 Size: 1753 Fragments arranged by size: 1753 1415 958 864 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5965 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2128 2447 2128 Size: 319 5646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3131 3235 4625 1239 Size: 1892 104 1390 2579 Fragments arranged by size: 2579 1892 1390 104 Bpu10I CC'TnA_GC Cuts at: 2780 2780 Size: 5965 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3115 4090 4955 5253 3115 Size: 975 865 298 3827 Fragments arranged by size: 3827 975 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4628 882 Size: 617 3129 2219 Fragments arranged by size: 3129 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2335 2620 2632 3112 3756 493 Size: 711 1131 285 12 480 644 2702 Fragments arranged by size: 2702 1131 711 644 480 285 12 BsaBI GATnn'nnATC Cuts at: 5959 5959 Size: 5965 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 2244 3772 4154 Size: 53 83 186 936 588 123 1528 382 Cuts at: 4154 4789 275 Size: 635 1451 Fragments arranged by size: 1528 1451 936 635 588 382 186 123 83 53 BsaWI w'CCGG_w Cuts at: 4398 4796 5070 5217 5628 4398 Size: 398 274 147 411 4735 Fragments arranged by size: 4735 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5740 Fragments arranged by size: 5740 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2771 1186 Size: 1054 531 4380 Fragments arranged by size: 4380 1054 531 BseYI C'CCAG_C Cuts at: 2014 2417 2438 2494 5168 5664 2014 Size: 403 21 56 2674 496 2315 Fragments arranged by size: 2674 2315 496 403 56 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2056 1163 Size: 573 320 5072 Fragments arranged by size: 5072 573 320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2118 3415 4176 5204 1540 Size: 275 186 117 1297 761 1028 2301 Fragments arranged by size: 2301 1297 1028 761 275 186 117 BsiHKAI G_wGCw'C Cuts at: 3054 3078 4026 4111 5182 3054 Size: 24 948 85 1071 3837 Fragments arranged by size: 3837 1071 948 85 24 BsmI GAATG_Cn' Cuts at: 3476 3569 3476 Size: 93 5872 BsmBI CGTCTCn'nnnn_ Cuts at: 2543 2543 Size: 5965 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2102 2196 2568 3054 3078 3284 Size: 399 267 45 94 372 486 24 206 Cuts at: 3284 4026 4111 5182 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 399 372 267 206 94 85 45 24 BspEI T'CCGG_A Cuts at: 5628 5628 Size: 5965 BspHI T'CATG_A Cuts at: 3854 5584 3854 Size: 1730 4235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3320 844 Size: 2476 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3852 1394 Size: 429 2029 3507 Fragments arranged by size: 3507 2029 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4460 4634 65 Size: 1183 3212 174 1396 Fragments arranged by size: 3212 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2117 2886 2978 4609 4796 1700 Size: 110 307 769 92 1631 187 2869 Fragments arranged by size: 2869 1631 769 307 187 110 92 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 3718 BssHII G'CGCG_C Cuts at: 1925 2611 1925 Size: 686 5279 BssSI C'ACGA_G Cuts at: 4025 5037 4025 Size: 1012 4953 BstAPI GCAn_nnn'nTGC Cuts at: 794 2079 2236 794 Size: 1285 157 4523 Fragments arranged by size: 4523 1285 157 BstBI TT'CG_AA Cuts at: 4769 4769 Size: 5965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5965 BstYI r'GATC_y Cuts at: 1972 2865 3246 3295 3662 4047 4064 5516 5602 Size: 893 381 49 367 385 17 1452 86 Cuts at: 5602 5614 5960 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 893 385 381 367 346 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2149 2350 513 Size: 552 939 145 201 4128 Fragments arranged by size: 4128 939 552 201 145 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2653 487 Size: 683 56 1427 3799 Fragments arranged by size: 3799 1427 683 56 BtsI GCAGTG_nn' Cuts at: 3481 4275 4295 3481 Size: 794 20 5151 Fragments arranged by size: 5151 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5930 DraI TTT'AAA Cuts at: 3090 3269 3090 Size: 179 5786 DraIII CAC_nnn'GTG Cuts at: 2079 2079 Size: 5965 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4972 809 Size: 507 3656 1802 Fragments arranged by size: 3656 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2115 2131 Size: 54 1425 50 275 18 213 72 16 Cuts at: 2131 3412 4301 5930 8 Size: 1281 889 1629 43 Fragments arranged by size: 1629 1425 1281 889 275 213 72 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2115 3412 1537 Size: 275 303 1297 4090 Fragments arranged by size: 4090 1297 303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2907 2948 3895 1353 Size: 1554 41 947 3423 Fragments arranged by size: 3423 1554 947 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2674 4552 4926 5072 387 Size: 1064 138 435 650 1878 374 146 1280 Fragments arranged by size: 1878 1280 1064 650 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3052 3052 Size: 5965 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2627 2756 2795 3131 3235 4028 4625 Size: 393 995 129 39 336 104 793 597 Cuts at: 4625 5412 5680 1239 Size: 787 268 1524 Fragments arranged by size: 1524 995 793 787 597 393 336 268 129 104 39 EcoNI CCTnn'n_nnAGG Cuts at: 2045 2363 2705 2045 Size: 318 342 5305 Fragments arranged by size: 5305 342 318 EcoO109I rG'GnC_Cy Cuts at: 2192 2426 2192 Size: 234 5731 EcoRI G'AATT_C Cuts at: 3274 3652 3274 Size: 378 5587 FspI TGC'GCA Cuts at: 4471 4471 Size: 5965 HaeII r_GCGC'y Cuts at: 1678 2124 2179 2735 5112 1678 Size: 446 55 556 2377 2531 Fragments arranged by size: 2531 2377 556 446 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3041 3073 4606 4638 4680 Size: 32 135 32 1469 32 1533 32 42 Cuts at: 4680 4712 1373 Size: 32 2626 Fragments arranged by size: 2626 1533 1469 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2575 3309 3555 669 Size: 988 281 637 734 246 3079 Fragments arranged by size: 3079 988 734 637 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5965 HpaI GTT'AAC Cuts at: 3555 3555 Size: 5965 KasI G'GCGC_C Cuts at: 2120 2120 Size: 5965 KpnI G_GTAC'C Cuts at: 2887 3290 2887 Size: 403 5562 MfeI C'AATT_G Cuts at: 3564 3564 Size: 5965 MluI A'CGCG_T Cuts at: 3258 5841 3258 Size: 2583 3382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5079 5263 1305 Size: 3774 184 2007 Fragments arranged by size: 3774 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4143 Fragments arranged by size: 4143 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2295 2474 2630 2906 3923 4282 4441 Size: 648 1129 179 156 276 1017 359 159 Cuts at: 4441 518 Size: 2042 Fragments arranged by size: 2042 1129 1017 648 359 276 179 159 156 MspA1I CmG'CkG Cuts at: 1606 2059 2070 2161 2233 2421 2455 2464 2494 Size: 453 11 91 72 188 34 9 30 Cuts at: 2494 2509 2562 2656 2788 2881 3381 4058 5206 Size: 15 53 94 132 93 500 677 1148 Cuts at: 5206 5451 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 500 453 245 188 132 94 93 91 72 53 34 30 15 11 9 NaeI GCC'GGC Cuts at: 2119 2980 2119 Size: 861 5104 NarI GG'CG_CC Cuts at: 2121 2121 Size: 5965 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4474 Fragments arranged by size: 4474 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5965 NgoMIV G'CCGG_C Cuts at: 2117 2978 2117 Size: 861 5104 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5965 NotI GC'GGCC_GC Cuts at: 3412 3412 Size: 5965 NruI TCG'CGA Cuts at: 2223 3066 2223 Size: 843 5122 NspI r_CATG'y Cuts at: 1132 1229 2344 2506 2629 2839 3323 4868 1132 Size: 97 1115 162 123 210 484 1545 2229 Fragments arranged by size: 2229 1545 1115 484 210 162 123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5965 PciI A'CATG_T Cuts at: 2625 4864 2625 Size: 2239 3726 PflMI CCAn_nnn'nTGG Cuts at: 1108 2164 1108 Size: 1056 4909 PfoI T'CCnGG_A Cuts at: 2303 2303 Size: 5965 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PmlI CAC'GTG Cuts at: 2620 2620 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4034 4066 5547 5579 4034 Size: 32 1481 32 4420 Fragments arranged by size: 4420 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5965 PsiI TTA'TAA Cuts at: 3131 3535 3131 Size: 404 5561 PspOMI G'GGCC_C Cuts at: 2192 2192 Size: 5965 PstI C_TGCA'G Cuts at: 830 2880 3083 3317 830 Size: 2050 203 234 3478 Fragments arranged by size: 3478 2050 234 203 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 PvuII CAG'CTG Cuts at: 2161 2233 2494 2161 Size: 72 261 5632 Fragments arranged by size: 5632 261 72 SacI G_AGCT'C Cuts at: 3054 3054 Size: 5965 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SbfI CC_TGCA'GG Cuts at: 3317 3317 Size: 5965 ScaI AGT'ACT Cuts at: 2027 4213 5925 2027 Size: 2186 1712 2067 Fragments arranged by size: 2186 2067 1712 SfcI C'TryA_G Cuts at: 826 1045 2876 3079 3313 4448 5129 5320 826 Size: 219 1831 203 234 1135 681 191 1471 Fragments arranged by size: 1831 1471 1135 681 234 219 203 191 SfoI GGC'GCC Cuts at: 2122 2122 Size: 5965 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5965 SmaI CCC'GGG Cuts at: 1872 3292 1872 Size: 1420 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3094 4069 4970 5232 820 Size: 197 926 1151 975 901 262 1553 Fragments arranged by size: 1553 1151 975 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 3323 1132 Size: 2191 3774 SspI AAT'ATT Cuts at: 5 52 3889 5 Size: 47 3837 2081 Fragments arranged by size: 3837 2081 47 StuI AGG'CCT Cuts at: 1517 2369 1517 Size: 852 5113 StyI C'CwwG_G Cuts at: 513 1065 2004 2751 3404 513 Size: 552 939 747 653 3074 Fragments arranged by size: 3074 939 747 653 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2452 3992 4009 4162 4347 4792 325 Size: 1255 872 1540 17 153 185 445 1498 Fragments arranged by size: 1540 1498 1255 872 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 2034 2343 Size: 274 80 33 51 1443 48 9 309 Cuts at: 2343 4211 5923 96 Size: 1868 1712 138 Fragments arranged by size: 1868 1712 1443 309 274 138 80 51 48 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5925 5957 5925 Size: 32 5933 Tth111I GACn'n_nGTC Cuts at: 1316 3200 1316 Size: 1884 4081 XbaI T'CTAG_A Cuts at: 3301 3301 Size: 5965 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5965 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5965 XmaI C'CCGG_G Cuts at: 1870 3290 1870 Size: 1420 4545 XmnI GAAnn'nnTTC Cuts at: 4094 4094 Size: 5965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI