(Linear) (Six-base) MAPSORT of: KIEE1142.seq Check: 722 from: 1 to: 1260 KIEE1142.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. Acc65I G'GTAC_C Cuts at: 0 878 1260 Size: 878 382 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 751 790 1260 Size: 622 129 39 470 Fragments arranged by size: 622 470 129 39 AflIII A'CryG_T Cuts at: 0 620 1253 1260 Size: 620 633 7 Fragments arranged by size: 633 620 7 AleI CACnn'nnGTG Cuts at: 0 290 1260 Size: 290 970 AlwNI CAG_nnn'CTG Cuts at: 0 231 489 1260 Size: 231 258 771 Fragments arranged by size: 771 258 231 ApaI G_GGCC'C Cuts at: 0 191 1260 Size: 191 1069 ApoI r'AATT_y Cuts at: 0 1218 1260 Size: 1218 42 AvaI C'yCGr_G Cuts at: 0 97 190 1260 Size: 97 93 1070 Fragments arranged by size: 1070 97 93 BanI G'GyrC_C Cuts at: 0 115 684 878 1260 Size: 115 569 194 382 Fragments arranged by size: 569 382 194 115 BanII G_rGCy'C Cuts at: 0 52 191 563 1049 1260 Size: 52 139 372 486 211 Fragments arranged by size: 486 372 211 139 52 BbeI G_GCGC'C Cuts at: 0 119 1260 Size: 119 1141 BbsI GAAGACnn'nnnn_ Cuts at: 0 1123 1260 Size: 1123 137 BbvCI CC'TCA_GC Cuts at: 0 775 1260 Size: 775 485 BlpI GC'TnA_GC Cuts at: 0 476 1260 Size: 476 784 Bme1580I G_kGCm'C Cuts at: 0 97 191 1260 Size: 97 94 1069 Fragments arranged by size: 1069 97 94 BmgBI CAC'GTC Cuts at: 0 588 1260 Size: 588 672 BmrI ACTGGGnnnn_n' Cuts at: 0 276 1260 Size: 276 984 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 123 442 1260 Size: 123 319 818 Fragments arranged by size: 818 319 123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1126 1230 1260 Size: 1126 104 30 Fragments arranged by size: 1126 104 30 Bpu10I CC'TnA_GC Cuts at: 0 775 1260 Size: 775 485 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1110 1260 Size: 1110 150 BsaAI yAC'GTr Cuts at: 0 330 615 627 1107 1260 Size: 330 285 12 480 153 Fragments arranged by size: 480 330 285 153 12 BsaHI Gr'CG_yC Cuts at: 0 116 239 1260 Size: 116 123 1021 Fragments arranged by size: 1021 123 116 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 235 766 1260 Size: 235 531 494 Fragments arranged by size: 531 494 235 BseYI C'CCAG_C Cuts at: 0 9 412 433 489 1260 Size: 9 403 21 56 771 Fragments arranged by size: 771 403 56 21 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 1260 Size: 51 1209 BsiEI CG_ry'CG Cuts at: 0 113 1260 Size: 113 1147 BsiHKAI G_wGCw'C Cuts at: 0 1049 1073 1260 Size: 1049 24 187 Fragments arranged by size: 1049 187 24 BsmBI CGTCTCn'nnnn_ Cuts at: 0 538 1260 Size: 538 722 Bsp1286I G_dGCh'C Cuts at: 0 52 97 191 563 1049 1073 1260 Size: 52 45 94 372 486 24 187 Fragments arranged by size: 486 372 187 94 52 45 24 BsrFI r'CCGG_y Cuts at: 0 112 881 973 1260 Size: 112 769 92 287 Fragments arranged by size: 769 287 112 92 BsrGI T'GTAC_A Cuts at: 0 338 1260 Size: 338 922 BssHII G'CGCG_C Cuts at: 0 606 1260 Size: 606 654 BstAPI GCAn_nnn'nTGC Cuts at: 0 74 231 1260 Size: 74 157 1029 Fragments arranged by size: 1029 157 74 BstYI r'GATC_y Cuts at: 0 860 1241 1260 Size: 860 381 19 Fragments arranged by size: 860 381 19 BtgI C'CryG_G Cuts at: 0 144 345 1260 Size: 144 201 915 Fragments arranged by size: 915 201 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 648 1260 Size: 648 612 DraI TTT'AAA Cuts at: 0 1085 1260 Size: 1085 175 DraIII CAC_nnn'GTG Cuts at: 0 74 1260 Size: 74 1186 EaeI y'GGCC_r Cuts at: 0 38 110 126 1260 Size: 38 72 16 1134 Fragments arranged by size: 1134 72 38 16 EagI C'GGCC_G Cuts at: 0 110 1260 Size: 110 1150 EarI CTCTTCn'nnn_ Cuts at: 0 902 943 1260 Size: 902 41 317 Fragments arranged by size: 902 317 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 669 1260 Size: 19 650 591 Fragments arranged by size: 650 591 19 EcoICRI GAG'CTC Cuts at: 0 1047 1260 Size: 1047 213 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 751 790 1126 1230 1260 Size: 622 129 39 336 104 30 Fragments arranged by size: 622 336 129 104 39 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 40 358 700 1260 Size: 40 318 342 560 Fragments arranged by size: 560 342 318 40 EcoO109I rG'GnC_Cy Cuts at: 0 187 421 1260 Size: 187 234 839 Fragments arranged by size: 839 234 187 HaeII r_GCGC'y Cuts at: 0 119 174 730 1260 Size: 119 55 556 530 Fragments arranged by size: 556 530 119 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1036 1068 1260 Size: 1036 32 192 Fragments arranged by size: 1036 192 32 HincII GTy'rAC Cuts at: 0 570 1260 Size: 570 690 KasI G'GCGC_C Cuts at: 0 115 1260 Size: 115 1145 KpnI G_GTAC'C Cuts at: 0 882 1260 Size: 882 378 MluI A'CGCG_T Cuts at: 0 1253 1260 Size: 1253 7 MslI CAynn'nnrTG Cuts at: 0 290 469 625 901 1260 Size: 290 179 156 276 359 Fragments arranged by size: 359 290 276 179 156 MspA1I CmG'CkG Cuts at: 0 54 65 156 228 416 450 459 489 Size: 54 11 91 72 188 34 9 30 Cuts at: 489 504 557 651 783 876 1260 Size: 15 53 94 132 93 384 Fragments arranged by size: 384 188 132 94 93 91 72 54 53 34 30 15 11 9 NaeI GCC'GGC Cuts at: 0 114 975 1260 Size: 114 861 285 Fragments arranged by size: 861 285 114 NarI GG'CG_CC Cuts at: 0 116 1260 Size: 116 1144 NgoMIV G'CCGG_C Cuts at: 0 112 973 1260 Size: 112 861 287 Fragments arranged by size: 861 287 112 NruI TCG'CGA Cuts at: 0 218 1061 1260 Size: 218 843 199 Fragments arranged by size: 843 218 199 NspI r_CATG'y Cuts at: 0 339 501 624 834 1260 Size: 339 162 123 210 426 Fragments arranged by size: 426 339 210 162 123 PciI A'CATG_T Cuts at: 0 620 1260 Size: 620 640 PflMI CCAn_nnn'nTGG Cuts at: 0 159 1260 Size: 159 1101 PfoI T'CCnGG_A Cuts at: 0 298 1260 Size: 298 962 PmlI CAC'GTG Cuts at: 0 615 1260 Size: 615 645 PsiI TTA'TAA Cuts at: 0 1126 1260 Size: 1126 134 PspOMI G'GGCC_C Cuts at: 0 187 1260 Size: 187 1073 PstI C_TGCA'G Cuts at: 0 875 1078 1260 Size: 875 203 182 Fragments arranged by size: 875 203 182 PvuII CAG'CTG Cuts at: 0 156 228 489 1260 Size: 156 72 261 771 Fragments arranged by size: 771 261 156 72 SacI G_AGCT'C Cuts at: 0 1049 1260 Size: 1049 211 ScaI AGT'ACT Cuts at: 0 22 1260 Size: 22 1238 SfcI C'TryA_G Cuts at: 0 871 1074 1260 Size: 871 203 186 Fragments arranged by size: 871 203 186 SfoI GGC'GCC Cuts at: 0 117 1260 Size: 117 1143 SmlI C'TyrA_G Cuts at: 0 1089 1260 Size: 1089 171 StuI AGG'CCT Cuts at: 0 364 1260 Size: 364 896 StyI C'CwwG_G Cuts at: 0 746 1260 Size: 746 514 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 447 1260 Size: 447 813 TatI w'GTAC_w Cuts at: 0 20 29 338 1260 Size: 20 9 309 922 Fragments arranged by size: 922 309 20 9 Tth111I GACn'n_nGTC Cuts at: 0 1195 1260 Size: 1195 65 Enzymes that do cut: Acc65I AcuI AflIII AleI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BsrGI BssHII BstAPI BstYI BtgI BtgZI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI KpnI MluI MslI MspA1I NaeI NarI NgoMIV NruI NspI PciI PflMI PfoI PmlI PsiI PspOMI PstI PvuII SacI ScaI SfcI SfoI SmlI StuI StyI TaqII TatI Tth111I Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglI BglII BmtI BsaI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DrdI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI MfeI MmeI MscI NcoI NdeI NheI NotI NsiI PacI PasI PmeI PpiI PpuMI PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XcmI XhoI XmaI XmnI ZraI